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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaI-2/cdaI-2 (cdaI-2, VI)
Affected Genes: CDAI (cell division arrest I)
Source References: PMID:18658256
Notes: Allelic to cdaI-1 (IA237) and cdaI-3 (IA282). Unequal fission-arrest following an anterior sliding of the oral primordium (39o C). Although 100% penetrant at 39o C., it is the least strongly expressed of the three known cdaI alleles; the anteriorly displaced division furrow often succeeds in completing cytokinesis, producing a small, often amacronucleate, anterior daughter cell.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01515 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding, not sure if complete
Source References: PMID:10831613
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01910 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpA-1/mpA-1 (mpA-1, VII)
Affected Genes: MPA (membranellar pattern A) Originally published as mp/mp
Source References: PMID:814035, PMID:818339
Notes: Abnormal oral apparatuses arising during oral replacement as a consequence of incomplete joining of two membranellar subfields. Not temperature-sensitive. Kaczanowski's original description was of non-mutagenized clones of the inbred D strain, which was accidentally homozygous for the naturally-occurring mpA-1 allele. IA280 is a derived offshoot, and shows very low expression in nutrient medium. Expression under culture conditions conducive to oral replacement was not tested.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01516 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; rts255/rts255 (mpr1-1; cy-s, gal-s, mp-r, IV)
Proper citation: RRID:TSC_SD00947 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wt (pm-r)
Affected Genes: WAG1 (TTHERM_00299879)
Notes: N-terminally tagged WAG1, completely assorted, neo3 cassette, B2086-based strain
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02055 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: rmm11[C3]/rmm11[C3] (mp-s, pm-s, rrm11, III)
Source References: PMID:8070657
Notes: rmm = rDNA maturation or maintenance mutation, located in the rDNA
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01640 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcr2[∆?::neo3]/dcr2[∆?::neo3] (DCR2, ?)
Affected Genes: DCR2 (TTHERM_00316190)
Genomic Alteration: Micronucleus: homozygous KO of DCR2 with neo3
Source References: PMID:15598983
Notes: Homozygous heterokaryon of ∆DCR2 Round I progeny of he∆SAI3 F2-6-pms-1
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02178 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi11[∆?::neo3] (twi11[∆?::neo3])
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: Neo 3 cassette replaces part of TWI11 coding region Macronucleus: Neo 3 cassette replaces part of TWI11 coding region
Notes: Homozygous homokaryon, partial coding seq. were replaced with neo3 cassette. Defective in mitosis
mates with TWI11 KO F2-23,
This strain was supposed to be pm-r but thawed pm-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02858 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1 (CHX1, MPR1; gal1-1; cy-s, mp-s, gal-R, IV)
Source References: PMID:7333457
Notes: Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01647 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcr1[∆?::neo3]/dcr1[∆?::neo3] (dcr1[∆?], ?)
Affected Genes: DCR1 (TTHERM_01216140)
Genomic Alteration: Micronucleus: homozygous KO of DCR1 with neo3 Macronucleus: Loss of part of DCR1 locus and of the neo3 used to KO.
Source References: PMID:15598983
Notes: Homozygous homokaryon of ∆DCR1. neo3 was deleted in Mac and assorted to pm-s. Can mate with c∆SAI1 3-7-1. DCR1 = SAI1.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01928 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-22; Mild basal body disorganization (TS)., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-22
Source References: PMID:21562224
Notes: Alanine scanning allele rescue of cen1∆ - Cen1 E22A, E23A, K25A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03273 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT2/hht2[3’neo2 ∆::(HHT3,GFPc)]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: C-terminal GFP-tag of HHT3, not sure if complete replacement, into HHT2 locus
Source References: PMID:16908532
Notes: Somatic replace HHT2 coding region with HHT3-GFP sequence. Not sure where the neo2 cassette is.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01896 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (twi11[∆?::neo3]; pm-r, VII)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Macronucleus: TWI11 with neo3
Notes: Somatic TWI 11 KO. Part of the coding sequence was replaced by neo3. In CU428
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02865 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (HTB1/htb1[∆::neo2]; htb2 [∆::neo2] assorted to pm-s so must have lost neo2; mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1
Source References: PMID:19822522
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2. Not mating type II,VI,VII.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02468 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,GFPc]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: C-terminal GFP- tag of HHT3, not sure if complete replacement
Source References: PMID:16908532
Notes: Somatic replace HHT3 gene with HHT3-GFP sequence. Neo2 in 5’ flanking of HHT3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01897 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆hht1 & ∆hht2 ∆hhf1 ∆hhf2
hht1[∆,neo2]/hht1[∆,neo2];
hhf1[∆,neo]/hhf1[∆,neo]; hhf2,hht2[∆, neo2]/ hhf2,hht2[∆, neo2] (hht1[∆,neo2]/hhf1[∆,neo]/hhf2,hht2[∆, neo2]/ chimeric H3[H3(1-75) H3.3(76-90) H3(91-135)]
MTT1/mtt1-?[5’ neo2,∆,(::CNA1), GFPc]; pm-r, bsr-1, hybrid H3 replaces major H3.
In MTT1 locus,GFP tagged CNA1)
Affected Genes: CNA1 (TTHERM_00146340), HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: ∆hht1, ∆hht2 ∆hhf1, ∆hhf2
Macronucleus: hybrid H3 replaces major H3.
In MTT1 locus, GFP tagged CNA1
Source References: PMID:15701804
Notes: major h3, h4 germline KO cells were rescued with hy3. Hybrid H3 contruct with H3(1-75) H3.3 (76-90) H3 (91-135), then transformed with Bsr-mtt1-cna1-gfp construct.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cas.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01813 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; MPR1[C3]/MPR1[C3]; PMR1[C3]PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Source References: PMID:8601476, PMID:8725229
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-s, pm-s, mat3.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01265 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1[C3]-1/pmr1[C3]-1;CHX1[C3]/CHX1[C3] (PMR1/PMR1 ;chx1-1/chx1-1; cy-r, pm-s, VII)
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01540 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r, II)
Affected Genes: TTHERM_00238830
Genomic Alteration: Macronucleus: V5 tagged PP36 protein
Source References: PMID:22426537
Notes: somatic mac transformation of B2086 with construct of PP36 (Protein phosphatase 36 kD MW, predicted TTHERM_00238830) V5 tag after ATG, with neo3 in reverse orientation -200 bases upstream of ATG. Localizes to nucleoli, not studied further.
Incomplete replacement, pushed to 3mg/ml pm with 0.5ug/ml Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02233 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU1/btu1[∆,::H4-neo]; MPR1-1/mpr1-1
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Micronucleus: H4-neo replaces coding of BTU1, heterozygous
Source References: PMID:9037049, PMID:10831613
Notes: Heterozygous germline transformant of BTUI knockout. Derived from Cu428 X B2086 transformed w/ BHAB-1(construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to BIKO-2.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02753 Copy
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