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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TTHERM_01084190-1[∆::NEO2] (WT; WT, ?)
Affected Genes: TTHERM_01084190 (BBS1)
Genomic Alteration: Macronucleus: none
Notes: Mates with UCB1002
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03310 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, III)
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01653 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MTT1/MTT1 (MTT1/mtt1-?[5’ neo2,∆,(::CNA1), GFPc]; Mtt1p/Cna1p-GFPc, II)
Affected Genes: CenpA
Genomic Alteration: Macronucleus: GFP cterminal tagged CNA1 coding, inserted into MTT1 locus
Source References: PMID:16738316
Notes: MTT-CenpA-GFP was transformed into B2086 at MTT1 locus somatially. Not completed - grow in 0.5 ug/mlCd++and 200ug/ml paromomycin.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01777 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cna1[∆::neo2]/cna1[∆::neo2] (?)
Affected Genes: CNA1 (TTHERM_00146340)
Genomic Alteration: Micronucleus: Neo2 into CNA1
Source References: PMID:16738316
Notes: Germline CenPA KO heterokaryon. CenPA coding is replaced by neo2. Non-star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01932 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1];hht2[GFPc]/hht2[GFPc] (HHT2-GFP,∆HHT3,∆HHT4; pm-r,bsr-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2 Macronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
Source References: PMID:16908532
Notes: H3.2-GFP homozygous heterokaryon is crossed with ∆3N4B heterokaryon, the heterozygotes then go through Round I and the * side and non* side are then mated to obtain the homozygous homokaryons.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01778 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: homozygous KO with neo cassette ∆HMGB/∆HMGB (pm-r, mp-?
)
Affected Genes: HMGB (TTHERM_00660180)
Notes: Obtained thru mating two homozygous germline KO strains. Complete replacement in both mic and mac. Same as -B2, independent strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01384 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r, II)
Affected Genes: TTHERM_00238830
Genomic Alteration: Macronucleus: V5 tagged PP36 protein
Source References: PMID:22426537
Notes: somatic mac transformation of B2086 with construct of PP36 (Protein phosphatase 36 kD MW, predicted TTHERM_00238830) V5 tag after ATG, with neo3 in reverse orientation -200 bases upstream of ATG. Localizes to nucleoli, not studied further.
Incomplete replacement, pushed to 3mg/ml pm with 0.5ug/ml Cd.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02234 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[∆::neo2]/htaz[∆::neo2] (mp-r?, II)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 knockout of HTAX, HTA1, HTAZ
Source References: PMID:19822522
Notes: HTA/HTZ1 triple KO heterokaryons;From mating of h∆∆HTA-G6A and h∆HTZ1-G311A1; made with B*VII.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02630 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (Mac∆TWI1::neo3, twi1[∆::neo3]; mpr1-1; pm-r, mp-r, ?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: Coding region of TWI1 replaced by neo3
Source References: PMID:12297043
Notes: B2086 X CU428 were transformed with TWI1 KO construct (whole coding was replaced by MTT-neo-BTU) and endogenous TWI1 gene was pushed out. Somatic transformant
this strain can mate with at least B2086, cu427, TWI1-KO4,-KO10
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02112 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 1L (CHX1; cy-s, VI)
Notes: Deletion on left arm of chromosome 1.
Proper citation: RRID:TSC_SD00734 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD00858 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2; CHX!/CHX1; mpr1?/mpr1? (mp-r, VII)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: Neo2 cassette knockout of HTAX coding region
Source References: PMID:11430834, PMID:12665578
Notes: HTA1 Germline knockout heterokaryon. Transformed CU428 X B2086 with pQR10B (removes all coding, replaces with neo cassette). It can mate with HTA1-G1 15B.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01394 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wt (eri3[∆?::neo3], II)
Affected Genes: ERI3 (TTHERM_00037140)
Genomic Alteration: Macronucleus: ∆Eri3 Complete KO with Neo3
Notes: 2.m02417 was expressed at low levels in growing and starved cells, and was up-regulated at 6hr of conjugation. Mating delayed.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02003 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: [PMS/PMS B or PMR/PMR C3]; Uni 2 (pmr1-1; cnj3, pm-r, VII)
Proper citation: RRID:TSC_SD00340 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (ngoA[3’neo3, GFPn]; pm-r, ?)
Affected Genes: NgoA (TTHERM_00655890)
Genomic Alteration: Macronucleus: Replace NgoA with n-terminal GFP tagged version with neo3 in 3’ flanking region of NgoA
Source References: PMID:15870266
Notes: somatic replaced Ngo A gene w/ its N terminal fusion w/ GFP copy. neo 3 cassette in NgoA 3’ flank
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02486 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; MPR[C3]/MPR[C3]; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3];MPR[C3]; PMR1[C3]; MAT3; cy-s, mp-s, pm-s, ?)
Source References: PMID:8601476, PMID:8725229
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01277 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[5’neo2,GFPc]/hht3[5’neo2,GFPc] (?)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: GFP tagged HHT3
Source References: PMID:16908532
Notes: non-star side of H3.3+GFP germline replacement heterokaryon.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01951 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HAn]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA tag of HHT3, not sure if complete replacement
Source References: PMID:16908532, PMID:9343391
Notes: HA-tagged hv2 transformant. Checked by western blot and immunofluorescent staining
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02646 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[5’neo3,HAn]/twi11[5’neo3,HAn] (twi11[5’neo3,HAn]; pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
Notes: Homozygous HA-tagged TWI 11 homokaryon.HA coding sequence was inserted just after the start codon of TWI11. neo3 cassette was inserted upstream of TWI11 coding
mates with HA-TWI11 F2-24.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02802 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; PMR1[C3]/PMR1[C3]; MAT2/MAT2 (CHX1[C3]; cy-s, ?)
Source References: PMID:8725229, PMID:8601476
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, pm-s, mat2. Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01281 Copy
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