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http://www.wormbase.org/db/get?name=WBStrain00047420

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119 (tm4063) III; wgIs797.
Notes: Made_by: DKV/MC|"Mutagen:Particle Bombardment"|"wgIs797 [flt-1::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44."

Proper citation: RRID:WB-STRAIN:WBStrain00047420 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00047422

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119 (tm4063) III; wgIs809.
Notes: Made_by: DKV/MC|"Mutagen:Particle Bombardment"|"wgIs809 [ets-5::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44."

Proper citation: RRID:WB-STRAIN:WBStrain00047422 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00047423

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119 (tm4063) III; wgIs810.
Notes: Made_by: DKV/MC|"Mutagen:Particle Bombardment"|"wgIs810 [C06A6.2::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44."

Proper citation: RRID:WB-STRAIN:WBStrain00047423 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00047435

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed4) III; syEx1539.
Notes: syEx1539 [nhr-246p::GFP + unc-119(+)]. Pick non-Unc to maintain. Dauer decision marker that indicates commitment to dauer formation in intestine and hypodermis. Reference: Shih, PY, et al., Dev Biol. 2019 Jun 23. pii: S0012-1606(18)30788-7.

Proper citation: RRID:WB-STRAIN:WBStrain00047435 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00047384

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:32255430, PMID:37728486, PMID:38177158
Synonyms: unc-119(ed3) III; qyIs46.
Notes: qyIs46 [emb-9p::emb-9::mCherry + unc-119(+)] X. Superficially wild-type with very low penetrance (~5%) rupture. Integrated collagen::mCherry reporter. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51.|"Supplementary_genotype [emb-9p::emb-9::mCherry + unc-119(+)] X"

Proper citation: RRID:WB-STRAIN:WBStrain00047384 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00047419

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119 (tm4063) III; wgIs782.
Notes: Made_by: DKV/MC|"Mutagen:Particle Bombardment"|"wgIs782 [ztf-14::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44."

Proper citation: RRID:WB-STRAIN:WBStrain00047419 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00047346

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: rcSi1 II; unc-119(ed3) III.
Notes: rcSi1 [mex-5p::rpt-1::mCherry + unc-119(+)] II. RPT-1::mCherry allows visualization of the proteasome in the germline. Reference: Sampuda KL, et al. BMC Cell Biol. 2017 Apr 19;18(1):18.

Proper citation: RRID:WB-STRAIN:WBStrain00047346 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00047721

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed3) III; czIs110.
Notes: czIs110 [mex-5p::GFP::KDEL::pie-1 3UTR + unc-119(+)]. GFP::KDEL is a marker of the luminal ER in the embryo. Reference: Lee et al., J Cell Biol. 2016 Sep 12;214(6):665-76.

Proper citation: RRID:WB-STRAIN:WBStrain00047721 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00047890

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: PMID:31242447
Synonyms: unc-119(ed4) III; syIs360.
Alternate IDs: CGC_PS7127
Notes: Batch Strain object requested via get_NS_ids.pl|"Generated from papers flagged positive during the last month for data type afp_strain/other_strain."|"syIs360 [ets-10p::GFP + unc-119(+)]. Dauer decision marker that indicates commitment to dauer formation in neurons and intestine. Reference: Shih, PY, et al., Dev Biol. 2019 Jun 23. pii: S0012-1606(18)30788-7."

Proper citation: RRID:WB-STRAIN:WBStrain00047890 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00048145

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:32650044
Synonyms: unc-119(ed3) III.
Notes: [2021-02-02T17:27:14.041Z WBPerson1983] New Strain: Genotype: unc-119(ed3) III Reference: WBPaper00059962

Proper citation: RRID:WB-STRAIN:WBStrain00048145 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00048564

Source Database: WormBase (WB)
Affected Genes: WBGene00003161(mdf-2)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00003161(mdf-2), WBGene00006843(unc-119)
Availability: available
Source References: PMID:31588029
Synonyms: unc-119(ed3) III; mdf-2(lt4::loxP::Cbr-unc-119(+)::loxP) IV/nT1 [qIs51] (IV;V).
Alternate IDs: CGC_OD2174
Notes: CRISPR/Cas9 engineered deletion of mdf-2 in which the mdf-2 coding sequence was replaced by unc-119. Heterozygotes are wild-type GFP+ and segregate mdf-2 null homozygotes (low brood size/embryonic lethal), wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Unknown if unc-119(ed3) from parental strain is still carried in the background. gRNA sequence: Gccaaattccccagttttag Reference: Lara-Gonzalez P, et al. Dev Cell. 2019 Nov 4;51(3):313-325.e10. doi: 10.1016/j.devcel.2019.09.005. PMID: 31588029.|"Generated from papers flagged positive during the last month for data type afp_strain/other_strain."

Proper citation: RRID:WB-STRAIN:WBStrain00048564 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00049146

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:32293562
Synonyms: unc-119(ed3) III; hrtSi28 IV.
Notes: Generated from afp_otherstrain Paper data WBPaper000595481.|"hrtSi28 [des-2p::mKate2::maph-1.1 + unc-119(+)] IV. hrtSi28 inserted into cxTi10882. Expression of mKate2::MAPH-1.1 microtubule marker in PVD and FLP. Reference: He L, et al. eLife 2020;9:e55111 doi: 10.7554/eLife.55111."

Proper citation: RRID:WB-STRAIN:WBStrain00049146 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00049949

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:33027250, PMID:37782660
Synonyms: ijmSi3 I; unc-119(ed3) III; ltIs37 IV.
Notes: Generated from papers flagged positive during the last month for data type afp_otherstrain and contains genotype and gene data.|"ijmSi3 [mex-5p::cls-2(re-encoded)::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] I. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. The coding sequence of GFP-tagged cls-2 in the transgene was re-coded using silent mutations to render it insensitive to RNAi-depletion of endogenous cls-2 expression. mCherry expression marks histones. Not known if unc-119(ed3) is still carried in the background of this strain. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Schlientz A and Bowerman B. PLoS Genet. 2020 Oct 7;16(10):e1008751. doi: 10.1371/journal.pgen.1008751."|"Made_by: Aleesa Schlientz"|"Supplementary_genotype ijmSi3 [pJD342/pJD330; ChrI_5mex-5_cls-2reenc::GFP_3tbb-2; cb-unc-119(+)]I; unc-119(ed3) III?; itIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV"

Proper citation: RRID:WB-STRAIN:WBStrain00049949 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00050174

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:34003969
Synonyms: vsSi32 unc-119(ed3) III.
Notes: Generated from papers flagged positive during the last month for data type afp_otherstrain and contains genotype and gene data.|"vsSi32 [goa-1::GFP + unc-119(+)] III. GOA-1::GFP translational fusion driven by 5kb of goa-1 promoter. Rescues unc-119 locomotion defect. vsSi32 is inserted into the left arm of chromosome III between sequences 5-tttactgcatactgaacaacaggggaaaagggg-3 and 5-tagaattagctgtaagacggcgtctaggttttgca-3. Reference: Kumar S, et al. G3 (Bethesda). 2021 Aug 7;11(8):jkab167. doi: 10.1093/g3journal/jkab167. PMID: 34003969"

Proper citation: RRID:WB-STRAIN:WBStrain00050174 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00059159

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:38872845
Synonyms: nicTi605[*oxTi612] unc-119(ed3) III.
Notes: Made_by: Katherine Yanagi|"nicTi605 [eft-3p::tdTomato::H2B::unc-119(-) [*oxTi612]] III. Broad, nuclear red fluorescence. Unc. Maintain at 20C, somewhat sick at 25C. Unc. nicTi605 is a modified version of oxTi612 that does not rescue unc-119(ed3). Broad, nuclear red fluorescence. This strain can be used for integration of multicopy transgenes using Fluorescent Landmark Interference (FLInt) and unc-119 rescue. Reference: Yanagi KS & Lehrbach N. MicroPublication Biology. 2024 (submitted)."|"[2024-05-24T15:13:05.597Z WBPerson557] New Strain: microPublication Strain: NJL4308 Genotype: nicTi605[eft-3p::tdTomato::H2B unc-119(-) *oxTi612] unc-119(ed3) III Reference: WBPaper00066814 Species: Caenorhabditis elegans"

Proper citation: RRID:WB-STRAIN:WBStrain00059159 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054628

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: Cbr-unc-119(stIs20000) III; stIs20022.
Notes: Made_by: Waterston lab|"stIs20022 [Cbr-his-72::GFP::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (GFP) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853863. doi: 10.1534/genetics.109.110270 PMID: 20008572."|"[2023-10-27T18:59:54.666Z WBPerson51134] New Strain: C. briggsae. CGC_data_submission"

Proper citation: RRID:WB-STRAIN:WBStrain00054628 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054699

Source Database: WormBase (WB)
Affected Genes: WBGene00000962(dhc-1)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00000962(dhc-1), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: dhc-1(lt45[dhc-1::GFP]) I; ltSi953 II; unc-119(ed3) III.
Notes: ltSi953 [mec-18p::vhhGFP4::ZIF-1::operon-linker::mKate2::tbb-2 3'UTR + Cbr-unc-119(+)] II. GFP tag inserted into the C-terminus of the endogenous dhc-1 locus using CRISPR-Cas9 engineering. Tissue-specific expression of GFP nanobody::ZIF-1 fusion promotes ubiquitylation and subsequent degradation of GFP-tagged dhc-1 protein in touch receptor neurons. Touch receptor neurons are red labeled with mKate2. Reference: Development. 2017 Jul 15;144(14):2694-2701. PMID: 28619826.

Proper citation: RRID:WB-STRAIN:WBStrain00054699 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054684

Source Database: WormBase (WB)
Affected Genes: WBGene00001423(fib-1)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001423(fib-1), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: muIs252 II; unc-119(ed3) III; fib-1(mu498[wrmScarlet11::fib-1]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::linker::fib-1 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054684 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054685

Source Database: WormBase (WB)
Affected Genes: WBGene00001877(his-3)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001877(his-3), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: muIs252 II; unc-119(ed3) III; his-3(mu500[his-3::wrmScarlet11(x3)]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged his-3::wrmScarlet11(x3) generated via CRISPR/Cas9 insertion of three wrmScarlet11 tags into the endogenous his-3 locus in parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054685 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054680

Source Database: WormBase (WB)
Affected Genes: WBGene00003230(mex-5)|WBGene00004896(smu-2)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00003230(mex-5), WBGene00004896(smu-2), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III.
Notes: Made_by: Dmytro Kukhtar|"Missense mutations D1135L and S1136W, G1218K and E1219Q, and R1335Q and T1337R were introduced on the Cas9 gene at EG9615 strain, to cause endogenous expression of the Cas9 variant SpG. SpG is efficient for CRISPR on NGN PAM sites. Reference: Vicencio J, et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4."

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•   Source: Integrated Animals