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http://www.wormbase.org/db/get?name=WBStrain00051673

Source Database: WormBase (WB)
Affected Genes: WBGene00001073(dpy-11)|WBGene00006761(unc-24)|WBGene00008140(xpf-1)|WBGene00016955(perm-5)
Genomic Alteration: WBGene00001073(dpy-11), WBGene00006761(unc-24), WBGene00008140(xpf-1), WBGene00016955(perm-5)
Availability: unknown
Source References: EMPTY
Synonyms: xpf-1(e1487) II; unc-24(e138) perm-5(t1932)/nT1[let (m435)] IV; dpy-11(e224)/nT1[let (m435)] V.
Notes: him-9 is other name for xpf-1|"Made_by: Vancouver KO Group"|"Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1932 homozygotes that produce dead embryos. GE2391 is also homozygous for him-9(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."|"Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1932 homozygotes that produce dead embryos. GE2391 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9."

Proper citation: RRID:WB-STRAIN:WBStrain00051673 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051713

Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:34865044
Synonyms: osIs158 II.
Notes: Made_by: Takefumi Negishi|"osIs158 [eft-3p::AtTIR1(F79G)::mRuby] II. Single copy insertion into ttTi5605 on LG II. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218."

Proper citation: RRID:WB-STRAIN:WBStrain00051713 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051679

Source Database: WormBase (WB)
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00001862(him-3)|WBGene00006768(unc-32)|WBGene00022803(ZK688.9)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00001862(him-3), WBGene00006768(unc-32), WBGene00022803(ZK688.9)
Availability: unknown
Source References: EMPTY
Synonyms: unc-32(e189) ZK688.9(t1587)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV.
Notes: Made_by: Vancouver KO Group|"Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1587 homozygotes that produce arrested embryos. GE2621 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."

Proper citation: RRID:WB-STRAIN:WBStrain00051679 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051678

Source Database: WormBase (WB)
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00001862(him-3)|WBGene00004075(pod-1)|WBGene00006768(unc-32)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00001862(him-3), WBGene00004075(pod-1), WBGene00006768(unc-32)
Availability: unknown
Source References: EMPTY
Synonyms: unc-32(e189) pod-1(t1674)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV.
Notes: Made_by: Vancouver KO Group|"Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1674 homozygotes that do not produce viable progeny. GE2605 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."

Proper citation: RRID:WB-STRAIN:WBStrain00051678 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051710

Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: gwIs39 III; bqSi225 IV; gwIs59.
Notes: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. bqSi225 [emr-1p::emr-1::mCherry + unc-119(+)] IV. gwIs59 [pha-4::mCherry::256xLacO::4xLexA + unc-119(+)]. Superficially wild-type. Express ubiquitous EMR-1::mCherry at the nuclear periphery. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. Worms have red intestine (all stages) and green intestine (from late L4 stage). Reference: Harr JC, et al. Genes Dev. 2020 Apr 1;34(7-8):560-579. PMID: 32139421.|"Made_by: Jennifer Harr"

Proper citation: RRID:WB-STRAIN:WBStrain00051710 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051717

Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: jsSi1579; wrdSi57 II.
Notes: wrdSi57 [^SEC^eft-3p:TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Pick Rollers to maintain. Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Self-excising cassette with Rol and HygroR markers still in strain to facilitate crosses. Heat-shock to remove SEC as described in Dickinson et al. 2015. jsSi1579 is an RMCE landing pad inserted at a sgRNA site 45 bp from the ttTi5605 insertion site. It contains an rpl-28p::GFP reporter flanked by FRT and FRT3 sites and a loxP site (for more details about landing pads, see Nonet, 2020.Genetics or visit https:

Proper citation: RRID:WB-STRAIN:WBStrain00051717 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051714

Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:34865044
Synonyms: ieSi58 IV; osIs182 V.
Notes: ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. osIs182 [eft-3p::AtTIR1(F79G) + LoxP + myo-2p::GFP + rps-27p::neoR + LoxP] V. ieSi58 is a single copy transgene inserted into chromosome IV (oxTi177) expressing degron::GFP in the soma. osIs182 is a single copy insertion of TIR1(F79G) into chromosome V (oxTi365) and expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218.|"Made_by: Takefumi Negishi"

Proper citation: RRID:WB-STRAIN:WBStrain00051714 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051719

Source Database: WormBase (WB)
Affected Genes: WBGene00001864(him-5)
Genomic Alteration: WBGene00001864(him-5)
Availability: unknown
Source References: EMPTY
Synonyms: qIs56 him-5(e1490) V; qEx557.
Notes: qIs56 [lag-2p::GFP + unc-119(+)] V. qEx557 [hsp-16p::ceh-22b + ttx-3::DsRed]. Him. Pick DsRed+ animals to maintain qEx557 array. Heat-shock can be used to drive the ectopic expression of CEH-22B (derived from Fire Lab vector pPD49.78). LAG-2::GFP is expressed the embryo, nerve cord, Z1/Z4, and DTCs. Reference: Lam N. et al. Curr Biol. 2006 Feb 7;16(3):287-95. doi: 10.1016/j.cub.2005.12.015. PMID: 16461282

Proper citation: RRID:WB-STRAIN:WBStrain00051719 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051661

Source Database: WormBase (WB)
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00001862(him-3)|WBGene00006768(unc-32)|WBGene00012713(bckd-1A)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00001862(him-3), WBGene00006768(unc-32), WBGene00012713(bckd-1A)
Availability: unknown
Source References: EMPTY
Synonyms: unc-32(e189) bckd-1A(t1461)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV.
Notes: Made_by: Vancouver KO Group|"Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1461 homozygotes that produce dead eggs. GE1742 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."

Proper citation: RRID:WB-STRAIN:WBStrain00051661 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051660

Source Database: WormBase (WB)
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00001862(him-3)|WBGene00006768(unc-32)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00001862(him-3), WBGene00006768(unc-32)
Availability: unknown
Source References: EMPTY
Synonyms: unc-32(e189) top-3(t1470)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV.
Notes: Made_by: Vancouver KO Group|"Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1470 homozygotes that produce arrested embryos. GE1735 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."

Proper citation: RRID:WB-STRAIN:WBStrain00051660 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051669

Source Database: WormBase (WB)
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00001862(him-3)|WBGene00004075(pod-1)|WBGene00006768(unc-32)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00001862(him-3), WBGene00004075(pod-1), WBGene00006768(unc-32)
Availability: unknown
Source References: EMPTY
Synonyms: unc-32(e189) pod-1(t1614)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV.
Notes: Made_by: Vancouver KO Group|"Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1614 homozygotes that do not produce viable progeny. GE2237 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."

Proper citation: RRID:WB-STRAIN:WBStrain00051669 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051701

Source Database: WormBase (WB)
Affected Genes: WBGene00002010(hsp-6)
Genomic Alteration: WBGene00002010(hsp-6)
Availability: unknown
Source References: PMID:39602251
Synonyms: hsp-6(mg585) V; mgIs73 V.
Notes: Made_by: Ruvkun|"mgIs73 [cyp-14A4p::GFP::cyp-14A4 3UTR + myo-2p::mCherry] V. Slow growth. Low brood size. Received as a replacement for GR2249. Reference: Mao K, et al. Cell Metab. 2019 Feb 14. pii: S1550-4131(19)30022-1."

Proper citation: RRID:WB-STRAIN:WBStrain00051701 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051667

Source Database: WormBase (WB)
Affected Genes: WBGene00001073(dpy-11)|WBGene00006761(unc-24)|WBGene00008140(xpf-1)|WBGene00019953(wapl-1)
Genomic Alteration: WBGene00001073(dpy-11), WBGene00006761(unc-24), WBGene00008140(xpf-1), WBGene00019953(wapl-1)
Availability: unknown
Source References: EMPTY
Synonyms: xpf-1(e1487) II; unc-24(e138) wapl-1(t1773)/nt1[let (m435)] IV; dpy-11(e224)/nt1[let (m435)] V.
Notes: him-9 is other name for xpf-1|"Made_by: Vancouver KO Group"|"Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1773 homozygotes that do not produce viable progeny. GE2153 is also homozygous for him-9(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."|"Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1773 homozygotes that do not produce viable progeny. GE2153 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9."

Proper citation: RRID:WB-STRAIN:WBStrain00051667 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051700

Source Database: WormBase (WB)
Affected Genes: WBGene00000485(che-3)
Genomic Alteration: WBGene00000485(che-3)
Availability: unknown
Source References: EMPTY
Synonyms: che-3(cas528[gfp::che-3(R1384C)]) I.
Notes: GFP inserted into the endogenous che-3 gene at its N-terminus and R1384C mutation introduced by Cas9-triggered homologous recombination. Lipophilic dye filling is superficially wild-type and the amphid and phasmid sensory cilia are mildly shortened without evident IFT particle accumulation.

Proper citation: RRID:WB-STRAIN:WBStrain00051700 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051666

Source Database: WormBase (WB)
Affected Genes: WBGene00001073(dpy-11)|WBGene00006761(unc-24)|WBGene00008140(xpf-1)|WBGene00013024(cept-2)
Genomic Alteration: WBGene00001073(dpy-11), WBGene00006761(unc-24), WBGene00008140(xpf-1), WBGene00013024(cept-2)
Availability: unknown
Source References: EMPTY
Synonyms: xpf-1(e1487) II; unc-24(e138)/nt1[let (m435)] IV; dpy-11(e224) cept-2(t2007)/nt1[let (m435)] V.
Notes: him-9 is other name for xpf-1|"Made_by: Vancouver KO Group"|"Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2007 homozygotes that produce dead eggs. GE2122 is also homozygous for him-9(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."|"Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2007 homozygotes that produce dead eggs. GE2122 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9."

Proper citation: RRID:WB-STRAIN:WBStrain00051666 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051705

Source Database: WormBase (WB)
Affected Genes: WBGene00012802(set-25)|WBGene00019883(met-2)
Genomic Alteration: WBGene00012802(set-25), WBGene00019883(met-2)
Availability: unknown
Source References: EMPTY
Synonyms: met-2(n4256) set-25(n5021) III; gwIs4 X.
Notes: gwIs4 [myo-3p::RFP + baf-1::GFP-lacI:::let-858 3'UTR] X. Worms are slow growing, with reduced brood size and become sterile at elevated temperatures. met-2 and set-25 mutants co-segregate with ~ 20cM distance. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. RFP expression in muscles. Reference: Towbin BD, et al. Cell. 2012 Aug 31;150(5):934-47. PMID: 22939621|"Made_by: Benjamin Towbin"

Proper citation: RRID:WB-STRAIN:WBStrain00051705 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051704

Source Database: WormBase (WB)
Affected Genes: WBGene00000123(ama-1)|WBGene00001074(dpy-13)
Genomic Alteration: WBGene00000123(ama-1), WBGene00001074(dpy-13)
Availability: unknown
Source References: EMPTY
Synonyms: gwIs39 III; dpy-13(eI84) ama-1(m118m251) IV; gwIs58.
Notes: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs58 [hsp-16.2p::mCherry::256xLacO::4xLexA + unc-119(+)]. Small transgene/large array. Slow growing and dumpy. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. Worms have green intestine (from late L4 stage). Might still carry unc-119(ed3) in background. Reference: Rohner S, et al. J Cell Biol. 2013 Mar 4;200(5):589-604. doi: 10.1083/jcb.201207024. PMID: 23460676

Proper citation: RRID:WB-STRAIN:WBStrain00051704 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051709

Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: xeSi302 II; gwIs114.
Notes: xeSi302 [nhx-2p::npp-9::GFP::BLRP::3xFLAG::unc-54 3'UTR + Cbr-unc-119(+)] II. gwIs114 [hsp-16.2p::hlh-1 + rol6(su1006)]. Intestine-specific expression of nuclear GFP reporter. Rollers have heat-shock-inducible expression of hlh-1 transcription factor. gwIs114 was generated using constructs provided by Michael W. Krause`s lab (NIDDK). Reference: Gonzalez-Sandoval A, et al. Cell. 2015 Dec 3;163(6):1333-47. doi: 10.1016/j.cell.2015.10.066. PMID: 26607792.

Proper citation: RRID:WB-STRAIN:WBStrain00051709 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051708

Source Database: WormBase (WB)
Affected Genes: WBGene00000415(ced-1)|WBGene00002989(lim-7)|WBGene00003004(lin-15)|WBGene00012802(set-25)|WBGene00019883(met-2)
Genomic Alteration: WBGene00000415(ced-1), WBGene00002989(lim-7), WBGene00003004(lin-15), WBGene00012802(set-25), WBGene00019883(met-2)
Availability: unknown
Source References: EMPTY
Synonyms: met-2(n4256) set-25(n5021) III; bcIs39 [lim-7p::ced-1::GFP + lin-15(+)] V.
Notes: Worms are slow growing, with reduced brood size and become sterile at elevated temperatures. CED-1::GFP used to detect apoptotic cells in germline. Reference: Zeller P, et al. Nat Genet. 2016 Nov;48(11):1385-1395. doi: 10.1038/ng.3672. PMID: 27668659.

Proper citation: RRID:WB-STRAIN:WBStrain00051708 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00051775

Source Database: WormBase (WB)
Affected Genes: WBGene00000898(daf-2)
Genomic Alteration: WBGene00000898(daf-2)
Availability: unknown
Source References: EMPTY
Synonyms: daf-2(hq61[daf-2::mNeongreen]) III.
Notes: Made_by: Yan-Ping Zhang, Wen-Hong Zhang|"mNeonGreen tag inserted at the 3' end of the endogenous daf-2 gene locus by CRISPR/Cas9 engineering. Phenotypic assays have shown that this mNeonGreen tag does not perturb the function of the DAF-2 protein. DAF-2::mNeonGreen is expressed in neurons, XXX cells, vulval cells, germ cells, and oocytes with clear plasma membrane localization as expected for a cell surface receptor. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https:"

Proper citation: RRID:WB-STRAIN:WBStrain00051775 Copy   

•   Source: Integrated Animals