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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 2L (CHX1; pmr1-1; cy-s, pm-r, II)
Notes: Deletion on left arm of chromosome 2.
Proper citation: RRID:TSC_SD00919 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2 (HTAX/htax[∆::neo2]; hta1[∆::neo2]; pm-r, mp-r, ?)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: KO of HTAX and HTA1 with neo2 Macronucleus: Complete KO of HTA1 with neo2, Partial replacement of HTAX with neo2
Notes: New batch, rescue of h∆∆HTA-A1F14A1 and B1G6A These are KO of the major HTA genes HTAX and HTA1 in the mic with HTAX partial in the mac. Not mating type II,V1I or VII;same as HTA1-B1a.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02388 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Notes: Collected by Paul Doerder on 5/21/2011 from the pond designated CRWP in PA (latitude 41.42, longitude -78.86)
Has a micronucleus. No mating, immature.
Proper citation: RRID:TSC_SD03112 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (Neo 2 KO of HHT1, WT HHT2/HHF2; pm-r, ?)
Affected Genes: HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo 2 KO of HHT1 and of HHT2/HHF2 Macronucleus: Neo 2 Ko of HHT1, WT HHT2/HHF2
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo in mic) assorted to Paromomycin sensitive in mac. Shoot with Yifan’s wildtype HHT2/HHF2 construct.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02544 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-2/janA-2; eja1-1/eja1-1 (janA-2; eja1-1; janA, eja1, V)
Affected Genes: JANA (janus A) (originally reported as jan); EJA1 (enhancer of janus)
Source References: PMID:1743393
Notes: A mutation that causes the dorsal surface to be converted to a partial mirror-image of the ventral surface and blocks conjugation. Allelic to janA-1.
Proper citation: RRID:TSC_SD00644 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[R40K,E445A, E446A,E447A]; pm-r, mp-?
, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with R40K, E445A, E446A, E447A and neo replacing coding of some ATU1 genes
Source References: PMID:10831613, PMID:9037049
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pR40AAA derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and R40K , E445A,E446A,E447A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02829 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht2[3’neo2,GFPc]/hht2[3’neo2,GFPc] (hht2[3’neo2,GFPc]; pm-r, ?)
Affected Genes: HHT1 (TTHERM_00189180)
Genomic Alteration: Micronucleus: GFP tagged HHT2 Macronucleus: GFP tagged HHT2
Source References: PMID:16908532
Notes: Round II progeny of crossing H3.2-GFP-3x-10 (box 72:7 and 6) heterokaryons. Selected by pm and homokaryon was obtained.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02791 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 5 [B or C3] (cnj3, pm-r, VII)
Proper citation: RRID:TSC_SD00372 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht2[3’ neo2,FLAGc]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: c-terminal HHT2 FLAG tagged
Source References: PMID:9343391, PMID:16908532
Notes: Don’t know if complete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02312 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-2/hpo1-2 (hpo1-2, VI)
Affected Genes: HPO1 (hypoangular)
Source References: PMID:8253268
Notes: A complex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. The phenotype is more strongly expressed at 28° C and less strongly at 39° than it is in hpo1-1 (IA393).
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01465 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2];hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT1 + HHT3 (TTHERM_00570560 + TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT3
Source References: PMID:16908532
Notes: homozygous, Neo cassette knockout.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01860 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: psmA-3/psmA-3 (psmA-3, II)
Affected Genes: PSMA (pseudomacrostome A)
Notes: Elongated phenotype with development of large oral-replacement or misplaced cell division oral primordia.(39°). Same phenotype as psmA-1 IV (IA223) but different conditions of expression: Detailed phenotypic analysis was carried out on the sister clone, IA486.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01467 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-1/cdaC-1; cdaE-2/cdaE-2 (cdaC-1; cdaE-2; cdaC, cdaE, IV)
Affected Genes: CDAC (cell division arrest C) , CDAE (cell division arrest E)
Source References: PMID:407114
Notes: Joint expression of cdaC and cdaE patterns of fission arrest.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01587 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: somatic, H1 wt-GFP tagged; pushed to completion (hho1[3’neo2,GFPc]; pm-r, GFP positive, VII)
Affected Genes: HHO1, Histone H1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: GFP tagged HHO1
Source References: PMID:12356861
Notes: This is the somatic replacement strain GFP tagged wt H1 replaced the endogenous H1 flanking neo2 cassette was used as resistance marker
From the Gorovsky Lab, University of Rochester.
Proper citation: RRID:TSC_SD01744 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htay[∆123-388::neo3]/htay[∆123-388::neo3] (HTAY/HTAY, ?)
Affected Genes: HTAY (TTHERM_01079200)
Genomic Alteration: Micronucleus: Neo3 ko of last 2/3 (AA123-388) of HTAY gene
Source References: PMID:22426537
Notes: Knockout Heterokaryon of HTAY gene. Different mating type from 6-2, 6-3, 6-6, 6-12. Same as 3-2-5
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02680 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpH-1/mpH-1 (mpH-1, IV)
Affected Genes: MPH (membranellar pattern H)
Source References: PMID:18658256
Notes: Non-conditional formation of two instead of three membranelles in the oral apparatus. Similar phenotype to mpG (IA352), but with lower penetrance of 2-membranellar phenotype.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01235 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: psmC-1/psmC-1 (psmC-1, II)
Affected Genes: PSMC (pseudomacrostome C)
Source References: PMID:18658256
Notes: Elongated phenotype with development of large oral-replacement or misplaced cell division oral primordia, especially in rich medium, at 39o C. The only known mutant allele of this gene. Located on chromosome 5. Expression is highly medium-sensitive as well as temperature-sensitive.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01514 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (dyh7::neo3; pm-r, ?)
Affected Genes: DYH7 (TTHERM_00912290)
Genomic Alteration: Macronucleus: dyh7::neo3
Notes: Submitted by Todd Hennessey, University at Buffalo
Proper citation: RRID:TSC_SD00704 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (?HHT1/hht1[∆::neo2; hht2[3’neo2, S10A]; Somatic ∆HHT1 with neo cassette and HHT2S10A (neo in 3 flank) cotransformant., VI)
Affected Genes: HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: KO of HHT1, HHT2 has mutation S10A
Source References: PMID:9636175, PMID:10199406
Notes: Single cell line from H3E transformant.
By antiphos H3 antibody staining, no stain so should be all HHT2 S10A. Also checked by Southern.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01887 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Source References: PMID:16908532, PMID:9343391
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01375 Copy
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