Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi111[∆?::neo3] (twi11[∆?::neo3]/twi111[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Source References: PMID:16738316
Notes: ∆TWI11 homokaryon transformed with Bsr-MTT1-CNA1-GFP.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01809 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 with GFP tagged H3 chimera protein 4, H3(1-79) H3.3(80-135); pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP tagged chimeric H3
Source References: PMID:16908532
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein 4, H3(1-79) H3.3(80-135), rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02580 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Nulli 2 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD01014 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (TWI1::HA-TWI1-neo3, VII)
Affected Genes: TWI1 (TTHERM_01161040)
Source References: PMID:12297043
Notes: CU428 was transformed with HA-TWI1-neo3 and endogenous TWI1 loci were completely replaced.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02068 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (GTU1/gtu1[3’neo2neo2,HAc], VII)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3’ flanking region
Source References: PMID:12356864
Notes: GTU (gamma tubulin) gene with c-terminal HA tag and two neo2 cassette inserted in 3’ flanking region. Partial replacement
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01413 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: RDR1/rdr1[∆::neo2] (?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Micronucleus: neo2 into RDR1
Notes: Cross RDP1del X B2086. No other information
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02509 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; gal1-1/gal1-1; Nulli 2,3,4,5 (CHX1[A]; GAL1[A]; MPR1[A]; cy-s, gal-s, mp-s, III)
Proper citation: RRID:TSC_SD00170 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HA tagged HTAY and V5 tagged PPO1; cy-r, pm-r, VII)
Affected Genes: PPO1 (TTHERM_00865290)
Genomic Alteration: Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag
Source References: PMID:22426537
Notes: HA2-HTAY cells transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles. Do give the correct ~36 kDa Western band for anti V5.
Grow with 15 ug/ml cy and 80 ug/ml pm.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02197 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, VII)
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01660 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi1[∆::neo3]/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 replaces coding of TWI1
Source References: PMID:12297043
Notes: Homozygous heterokaryon of TWI1 gene with neo3, derived from B2086XCU428. Can mate with ∆TWI1hk-C4 and CU427.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02116 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1-1/pmr1-1; cnj5-1/cnj5-1 (cnj5-1; pmr1-1; cnj5, pm-r, II)
Affected Genes: CNJ5
Source References: PMID:9299115
Proper citation: RRID:TSC_SD00218 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BTU11-428,BLT1428-473))], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pbBc-1 construct. BTU1(AA 1-428) with BLT at Cterminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02242 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (btu1-? [∆2,3,4] ; BTU2/btu2-?[∆::neo1], ?)
Affected Genes: BTU (TTHERM_00348510)1, BTU2 (TTHERM_00836580)
Genomic Alteration: Macronucleus: BTU1 has the REI removed from the MREI at start of protien, BTU2 partically replaced by btu2::neo1
Notes: Original strain B6-D(VB1 cells with BTU1,MREI has REI deleted but rest of BTU1 is WT, the VB1 mutation L350 to M350 is back to L350 so no longer hyper sensitive to taxol) This cell was somatically transformed. Need to check if all BTU1 is replaced.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02481 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr13-1[∆::NEO2] (sfr13-1[∆::NEO2]; Reduced cell size, defective oral apparatus, fewer basal bodies, basal body stability defect, basal body separation and reorientation defect., ?)
Affected Genes: SFR13 (TTHERM_00522830)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2]
Source References: PMID:23426847
Notes: sfr13 complete null.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03296 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆BTU1/∆BTU1, ∆BTU2/∆BTU2, chx+/chx+,Mpr?/Mpr? (mp-r)
Affected Genes: BTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580)
Notes: BTU1,BTU2 double germline knockout heterokaryon originally from Jacek Gaertig. ∆BTU1 by neo cassette, ∆BTU2 by blasticidin(BSR) cassette. It can mate with DB6B, received from Jacek Gaertig, 3/8/00.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02001 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta2[∆::neo2]/hta2[∆::neo2]; mpr-1/mpr-? (mp-r, ?)
Affected Genes: HTA2 ( TTHERM_00316500 )
Genomic Alteration: Micronucleus: Neo 2 into HTA1, replaces coding
Notes: CU428xB2086 HTA2 germline knockout heterokaryon transform with pQR17, remove all coding by neo. Can mate with G209A1 and G209C4.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01398 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[5’neo2,GFPc]/hht3[5’neo2,GFPc] (?)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: GFP tagged HHT3
Source References: PMID:16908532
Notes: non-star side of H3.3+GFP germline replacement heterokaryon.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01950 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[5’neo3,HAn]/twi11[5’neo3,HAn] (twi11[5’neo3,HAn]; pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
Notes: Homozygous HA-tagged TWI 11 homokaryon.HA coding sequence was inserted just after the start codon of TWI11. neo3 cassette was inserted upstream of TWI11 coding.
mates wtih F2-5,F2-8.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02801 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: disA-1/disA-1 (disA-1; disA, VII)
Affected Genes: DISA (disorganized A)
Source References: PMID:7781905, PMID:17246135
Notes: disA-1 is a partially ts mutation that brings about disorganization of ciliary rows, without affecting the positioning of major cortical landmarks, or cytokinesis.
Proper citation: RRID:TSC_SD00629 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K4Q] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4Q
Source References: PMID:19822522
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-K4Q.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02090 Copy
Can't find your Organism?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific organism, it's easier to enter an RRID or a Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your organism in the search results, please help us by registering it into the system — it's easy. Organisms identifiers are registered through multiple sources depending on the species:
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
