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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, II)
Affected Genes: HHT1 (TTHERM_00570560)
Genomic Alteration: Micronucleus: Neo KO of HHT1
Source References: PMID:15701804
Notes: The germline knockout heterokaryon of HHT1 gene with neo cassette completely replacing coding region. Three independent cell lines #1,#2,# 3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02621 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (twi11[∆?::neo3]; pm-r, VII)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Macronucleus: TWI11 with neo3
Notes: Somatic TWI 11 KO. Part of the coding sequence was replaced by neo3. In CU428
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02864 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Source References: PMID:8601476, PMID:8725229
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, pm-s, mat3. Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01259 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HHT3?/htt3[5’neo2, ∆, (::HHT2,GFPc)]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: HHT2 with GFP c-terminal tag replaces the HHT3 coding region
Source References: PMID:16908532
Notes: somatic replace H3.3 coding with H3.2+GFP, neo 2 cassette in 5’ flank of H3.3. Not sure if complete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01899 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (pm-r, VII)
Affected Genes: MTT1 (TTHERM_00241640)
Genomic Alteration: Macronucleus: Neo coding replaces MTT1 coding of some of MTT1 copies, both present
Source References: PMID:11891286
Notes: CU428 transformed by pTTMN construct and cells selected with 10 mg/ml paromomycin. Partially replaced. Still has wt Metallothionein gene copies in mac as well as other alleles with neo coding.
Proper citation: RRID:TSC_SD01419 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-EF1-EF2; Basal body disorganization (TS), ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-EF1-EF2
Source References: PMID:21562224
Notes: EF-hand mutant allele rescue of cen1∆ - D36A, D72A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03286 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1 (CHX1, MPR1; gal1-1; cy-s, mp-s, gal-R, IV)
Source References: PMID:7333457
Notes: Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01663 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Source References: PMID:8754831, PMID:8760889
Notes: CET of CU428 x B2086 with plamid XL202, HTA2 with neo2 cassette replacing coding
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02878 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (btu1-? [∆2,3,4] ; BTU2/btu2-?[∆::neo1], ?)
Affected Genes: BTU (TTHERM_00348510)1, BTU2 (TTHERM_00836580)
Genomic Alteration: Macronucleus: BTU1 has the REI removed from the MREI at start of protien, BTU2 partically replaced by btu2::neo1
Notes: Original strain B6-D(VB1 cells with BTU1,MREI has REI deleted but rest of BTU1 is WT, the VB1 mutation L350 to M350 is back to L350 so no longer hyper sensitive to taxol) This cell was somatically transformed. Need to check if all BTU1 is replaced
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02482 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht2[3’neo2, ∆::(HHT3,GFPc)]/hht2[3’neo2, ∆::(HHT3,GFPc)] (?HHT2/hht2[3’neo2 ∆::(HHT3,GFPc)], ?)
Affected Genes: HTT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: C-terminal GFP- tag of HHT3, into HHT2 locus
Source References: PMID:16908532
Notes: germline H3.3 +GFP at H3.2, heterokaryon. lnon-star sideof H3.3+GFP germline replacement at H3.2 locus.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01953 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Del 4 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD00987 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Source References: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01957 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (MTT1/mtt1-?[5’neo4,∆,HAHAn(::TCD2)]; pm-r, II)
Affected Genes: HPL7 (TTHERM_00551070)
Genomic Alteration: Macronucleus: Two HA tags after ATG of TCD2, neo4 in 5’ flank, inserted into MTT1 locus for over expression, need Cd
Notes: HA2-TCD2 overexpession from MTT1 locus, neo 4 upstream. HA-Tcd2p localizes to micronuclei in Growing and conjugation stage.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02495 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1/mpr1-1 (MPR1; cy-s, mp-s, tr, VII)
Proper citation: RRID:TSC_SD00075 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (tcd3-tcd4-1[∆?::neo4]; pm-r, II)
Affected Genes: HPL4 (TTHERM_00585180), HPL5 (TTHERM_00585190)
Genomic Alteration: Macronucleus: Used one neo4 construct to KO both adjacent TCD3 and TCD4
Notes: TCD3 and TCD4 gene were knock out from Mac with one construct. cell growing and development is normal.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02532 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 3 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD00993 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaI-I/cdaI-1 (cdaI-1, II)
Affected Genes: CDAI (cell division arrest I)
Source References: PMID:18658256
Notes: Temperature-sensitive expression; fully penetrant at 39 degrees with high expressivity. Allelic to cdaI-2 (IA251) and cdaI-3 (IA282). Unequal fission-arrest following an anterior sliding of the oral primordium.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01449 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: exoB2/exoB2; chx1-1/CHX1-1 (exoB2; CHX1; exo-; cy-s, ?)
Source References: PMID:1499157
Notes: Submitted by Eileen Hamilton, University of California at Santa Barbara
Proper citation: RRID:TSC_SD01723 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 1R (CHX1; pmr1-1; cy-s, pm-r, II)
Notes: Deletion on right arm of chromosome 1.
Proper citation: RRID:TSC_SD00755 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaD-1/cdaD-1 (cdaD-1; cdaD, V)
Affected Genes: CDAD (cell division arrest D). Originally reported as mo6.
Source References: PMID:17246135, PMID:407114, PMID:821816
Notes: cdaD-1 causes temperature sensitive cell division arrest. Allelic with cdaD-2 (IA134). Located on chromosome 5. A detailed phenotypic analysis was carried out on a homozygous clone of a preceding sexual generation, which is now extinct.
Proper citation: RRID:TSC_SD00639 Copy
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