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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00062657
Source Database: WormBase (WB)
Affected Genes: WBGene00013352(lon-8)
Genomic Alteration: WBGene00013352(lon-8)
Availability: unknown
Source References: EMPTY
Synonyms: lon-8(wrd305 wrd317[lon-8::mScarlet::2xOLLAS]) V.
Notes: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous lon-8 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd305.
Proper citation: RRID:WB-STRAIN:WBStrain00062657 Copy
http://www.wormbase.org/db/get?name=WBStrain00062658
Source Database: WormBase (WB)
Affected Genes: WBGene00011522(srap-1)
Genomic Alteration: WBGene00011522(srap-1)
Availability: unknown
Source References: EMPTY
Synonyms: srap-1(wrd318[srap-1::mNG::3xFLAG]) II.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted in the first exon after the signal sequenceof the endogenous srap-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062658 Copy
http://www.wormbase.org/db/get?name=WBStrain00062656
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: ctsa-1.1(wrd303 wrd315[ctsa-1.1::mScarlet::2xOLLAS]) II.
Notes: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous ctsa-1.1 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd303.
Proper citation: RRID:WB-STRAIN:WBStrain00062656 Copy
http://www.wormbase.org/db/get?name=WBStrain00062648
Source Database: WormBase (WB)
Affected Genes: WBGene00000699(col-125)
Genomic Alteration: WBGene00000699(col-125)
Availability: unknown
Source References: EMPTY
Synonyms: col-125(wrd300[col-125::mNG::3xFLAG]) IV.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-125 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062648 Copy
http://www.wormbase.org/db/get?name=WBStrain00062640
Source Database: WormBase (WB)
Affected Genes: WBGene00000601(col-12)
Genomic Alteration: WBGene00000601(col-12)
Availability: unknown
Source References: EMPTY
Synonyms: col-12(wrd279[col-12::mNG::3xFLAG]) V.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-12 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062640 Copy
http://www.wormbase.org/db/get?name=WBStrain00062641
Source Database: WormBase (WB)
Affected Genes: WBGene00000618(col-41)
Genomic Alteration: WBGene00000618(col-41)
Availability: unknown
Source References: EMPTY
Synonyms: col-41(wrd280[col-41::mNG::3xFLAG]) X.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted 6 bp before the stop codon in the endogenous col-41 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062641 Copy
http://www.wormbase.org/db/get?name=WBStrain00062647
Source Database: WormBase (WB)
Affected Genes: WBGene00010723(cpg-7)
Genomic Alteration: WBGene00010723(cpg-7)
Availability: unknown
Source References: EMPTY
Synonyms: cpg-7(wrd287[cpg-7:::mNG::3xFLAG]) II.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous cpg-7 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062647 Copy
http://www.wormbase.org/db/get?name=WBStrain00062639
Source Database: WormBase (WB)
Affected Genes: WBGene00021095(mlt-8)
Genomic Alteration: WBGene00021095(mlt-8)
Availability: unknown
Source References: EMPTY
Synonyms: mlt-8(wrd278[mlt-8::mNG::3xFLAG]) II.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous mlt-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062639 Copy
http://www.wormbase.org/db/get?name=WBStrain00062738
Source Database: WormBase (WB)
Affected Genes: WBGene00003622(nhr-23)
Genomic Alteration: WBGene00003622(nhr-23)
Availability: unknown
Source References: EMPTY
Synonyms: nhr-23(kry61[nhr-23::AID*::TEV::3xFLAG]) I; ieSi57 II.
Notes: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Strain for somatic depletion of NHR-23::AID*::TEV::3xFLAG using the auxin-inducible degron system. Derived by crossing parental strains KRY87 and CA1200. Reference: Zhang L, et al. Development. 2015 Dec 15;142(24):4374-84. doi: 10.1242/dev.129635. PMID: 26552885.
Proper citation: RRID:WB-STRAIN:WBStrain00062738 Copy
http://www.wormbase.org/db/get?name=WBStrain00062694
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00001595(gld-1)
Genomic Alteration: WBGene00000254(bli-4), WBGene00001595(gld-1)
Availability: unknown
Source References: EMPTY
Synonyms: gld-1(q1243) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Notes: Pick GFP+ to maintain. CRIPSR-engineered modification of gld-1 FBEa and FBEa* in gld-1 3'UTR. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1243 homozygotes (sterility/reduced fertility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Increase in distal GLD-1 protein levels and decrease in proximal GLD-1 protein levels. Qiu et al., in preparation.
Proper citation: RRID:WB-STRAIN:WBStrain00062694 Copy
http://www.wormbase.org/db/get?name=WBStrain00062698
Source Database: WormBase (WB)
Affected Genes: WBGene00001072(dpy-10)|WBGene00001401(fbf-1)|WBGene00001402(fbf-2)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00001401(fbf-1), WBGene00001402(fbf-2)
Availability: unknown
Source References: EMPTY
Synonyms: fbf-1(ok91) fbf-2(q1261[*q973])/mIn1 [mIs14 dpy-10(e128)] II.
Notes: Made_by: Brian Carrick|"Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered (H453A H454A E457A) substitutions. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (Sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain."
Proper citation: RRID:WB-STRAIN:WBStrain00062698 Copy
http://www.wormbase.org/db/get?name=WBStrain00062735
Source Database: WormBase (WB)
Affected Genes: WBGene00003623(nhr-25)
Genomic Alteration: WBGene00003623(nhr-25)
Availability: unknown
Source References: EMPTY
Synonyms: nhr-25(kry59[nhr-25::AID*::TEV::3xFLAG]) X.
Notes: AID*::TEV::3xFLAG tag inserted at the C-terminus of the endogenous nhr-25 locus by CRISPR. Allele obtained by pha-1 co-conversion, following Ward 2015 method. Reference: Zhang L, et al. Development. 2015 Dec 15;142(24):4374-84. doi: 10.1242/dev.129635. PMID: 26552885.
Proper citation: RRID:WB-STRAIN:WBStrain00062735 Copy
http://www.wormbase.org/db/get?name=WBStrain00062733
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: louIs10.
Notes: louIs10 [myo-3p::sqst-1::mCherry::GFP::unc-54 3'UTR]. Tandemly-tagged p62/sequestosome marker expressed in the muscle can be used to monitor autophagic activity. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394.|"Made_by: Adam Bohnert"
Proper citation: RRID:WB-STRAIN:WBStrain00062733 Copy
http://www.wormbase.org/db/get?name=WBStrain00062680
Source Database: WormBase (WB)
Affected Genes: WBGene00000770(cpb-1)|WBGene00003785(nos-3)
Genomic Alteration: WBGene00000770(cpb-1), WBGene00003785(nos-3)
Availability: unknown
Source References: EMPTY
Synonyms: cpb-1(q967[nos-3::1xOLLAS]) III.
Notes: 1xOLLAS tag inserted into endogenous nos-3 locus after L141 within a non-conserved region near the N-terminus.|"Made_by: Brian Carrick"
Proper citation: RRID:WB-STRAIN:WBStrain00062680 Copy
http://www.wormbase.org/db/get?name=WBStrain00062686
Source Database: WormBase (WB)
Affected Genes: WBGene00003083(lst-1)
Genomic Alteration: WBGene00003083(lst-1)
Availability: unknown
Source References: EMPTY
Synonyms: lst-1(q1124[*q1004]).
Notes: 3xV5 epitope tag inserted into C-teminus of endogenous lst-1 locus with PUF-interacting motif A disrupted (PIM A mutant). Reference: Haupt KA, et al. 2019 Oct 17;146(20):dev181644. PMID: 31515205.|"Made_by: A. Ferdous"
Proper citation: RRID:WB-STRAIN:WBStrain00062686 Copy
http://www.wormbase.org/db/get?name=WBStrain00062687
Source Database: WormBase (WB)
Affected Genes: WBGene00012104(sygl-1)
Genomic Alteration: WBGene00012104(sygl-1)
Availability: unknown
Source References: EMPTY
Synonyms: sygl-1(q1167[*q1135])) I.
Notes: C-teminal V5 epitope tag inserted into endogenous sygl-1 locus that has a CRISPR-engineered mutation of predicted Notch-dependent cis-regulatory elements LBS BCD (Yoo et al., 2004). Reference: Lynch TR, et al. Development. 2022 Apr 1;149(7):dev200332. PMID: 35394007.|"Made_by: T. Lynch"
Proper citation: RRID:WB-STRAIN:WBStrain00062687 Copy
http://www.wormbase.org/db/get?name=WBStrain00062720
Source Database: WormBase (WB)
Affected Genes: WBGene00006977(zif-1)|WBGene00044431(mzt-1)
Genomic Alteration: WBGene00006977(zif-1), WBGene00044431(mzt-1)
Availability: unknown
Source References: EMPTY
Synonyms: mzt-1(wow51[GFP::3xFLAG::mzt-1]) I; zif-1(gk117) III.
Notes: GFP and 3xFLAG tags inserted into endogenous mzt-1 locus. No overt phenotypes. GFP fluorescence is observed at microtubule-organizing centers. Reference: Sallee M, et al. PLoS Biol. 2018 Aug 6;16(8):e2005189. doi: 10.1371/journal.pbio.2005189. PMID: 30080857.|"Made_by: Jenny Zonka"
Proper citation: RRID:WB-STRAIN:WBStrain00062720 Copy
http://www.wormbase.org/db/get?name=WBStrain00062724
Source Database: WormBase (WB)
Affected Genes: WBGene00016741(haps-1)
Genomic Alteration: WBGene00016741(haps-1)
Availability: unknown
Source References: EMPTY
Synonyms: haps-1(ljt1) I.
Notes: CRISPR-engineered KO strain of haps-1 made by knock-in of tandem stop codons to the first exon of endogenous haps-1 locus. haps-1(C48B6.3) is the C. elegans ortholog of human HAPSTR1. haps-1(ljt1) is superficially wild-type when cultured at 20C, but is hypersensitive to paraquat (redox stress), MLN4924 (neddylation inhibition), and camptothecin (genotoxic stress). Reference: Amici DR, et al. Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2111262119. doi: 10.1073/pnas.2111262119. PMID: 35776542.
Proper citation: RRID:WB-STRAIN:WBStrain00062724 Copy
http://www.wormbase.org/db/get?name=WBStrain00062689
Source Database: WormBase (WB)
Affected Genes: WBGene00001597(gld-3)
Genomic Alteration: WBGene00001597(gld-3)
Availability: unknown
Source References: EMPTY
Synonyms: gld-3(q1215[*q1065]) II.
Notes: 1xV5 tag inserted into endogenous gld-3 locus containing K864A & L867A engineered mutations, specifically tagging GLD-3L isoform. GLD-3L::1xV5 (K864A, L867A) animals are 30% Fog. Derived by CRISPR-engineered mutation of parental strain JK6091.|"Made_by: Brian Carrick"
Proper citation: RRID:WB-STRAIN:WBStrain00062689 Copy
http://www.wormbase.org/db/get?name=WBStrain00062722
Source Database: WormBase (WB)
Affected Genes: WBGene00004953(spd-2)|WBGene00004955(spd-5)
Genomic Alteration: WBGene00004953(spd-2), WBGene00004955(spd-5)
Availability: unknown
Source References: EMPTY
Synonyms: spd-5(wow36[tagRFP-T::spd-5]) spd-2(wow60[spd-2::GFP::3xFlag]) I.
Notes: Made_by: Jeremy Magescas|"tagRFP-T tag inserted into endogenous spd-5 locus. GFP and 3xFLAG tags inserted into endogenous spd-2 locus. No overt phenotypes. GFP and RFP fluorescence is observed at centrosomes. Reference: Magescas J, et al. eLife. 2019 Jun 27;8:e47867. doi: 10.7554/eLife.47867. PMID: 31246171."
Proper citation: RRID:WB-STRAIN:WBStrain00062722 Copy
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