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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1[C3]-1/pmr1[C3]-1 (PMR1[C3]; pm-s, I)
Notes: C3 strain - functional heterokaryon carrying paromomycin resistance in micronucleus.
Proper citation: RRID:TSC_SD00663 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: exoB2/exoB2; gal1-1/gal1-1 (exoB2, gal1-1; exo-, gal-r, III)
Source References: PMID:3912402, PMID:9380694, PMID:15752136, PMID:19684282, PMID:1499156, PMID:1499157, PMID:2065648, PMID:11779800
Proper citation: RRID:TSC_SD00666 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-4/cdaC-4 (cdaC-4, IV)
Affected Genes: CDAC (cell division arrest C) Originally published as mo3d
Source References: PMID:821816
Notes: Failure to complete cytokinesis accompanied by cell elongation (39o C). Allelic to cdaC-1 (IA-119) and cdaC-2 (IA-123). Generates 100% fission blockage at 39o C. but not at lower temperatures.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01512 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TWI1/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, heterozygous
Source References: PMID:12297043
Notes: Heterozygous heterokaryon of TWI1 KO, can mate with ∆TWI1 hehe C4-4-3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02848 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (HTA2 has neo2; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes Macronucleus: WT HTAX
Source References: PMID:12665578, PMID:17242195
Notes: It is HTA1 WT somatic rescue strain of HTA1,HTA2 double knockout. Can mate with 1-3(2).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02170 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAZ/htaz[5’neo2,GFPc]; pm-r, VII)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Macronucleus: GFP tagged HTAZ
Source References: PMID:11430834, PMID:12665578
Notes: Somatic transformation with pQR88, (GFP)n-hv1. Neo inserted at 5’flanking. Only a partial replacement-> lethal. Cell turns green altough not a complete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02454 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1,K192A, R193A), HAc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K192A R193A and C-terminal HA tag into the MTT1 locus, partial replacement.
Source References: PMID:16344310, PMID:12356864
Notes: pGTU5 HA rescue of GTUKO 5x6 progeny, having mutation at K192A R193A in GTU1-1 gene.In the MTT1 locus. HA c-terminal tag.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02058 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcr2[∆?::neo3]/dcr2[∆?::neo3] (DCR2, ?)
Affected Genes: DCR2 (TTHERM_00316190)
Genomic Alteration: Micronucleus: homozygous KO of DCR2 with neo3
Source References: PMID:15598983
Notes: Homozygous heterokaryon of ∆DCR2 Round I progeny of he∆SAI3 F2-2-pms-1.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02177 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HA tagged HTAY and V5 tagged PPO1; cy-r, VII)
Affected Genes: PPO1 (TTHERM_00865290)
Genomic Alteration: Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag
Source References: PMID:22426537
Notes: Xiaoyuan’s HA2-HTAY cells transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles. Do give the correct ~36 kDa Western band for anti V5.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02733 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2]; mpr1-1/mpr1-1 (GTU1,mpr1-1; mp-r, VI)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 replaces coding of GTU1
Source References: PMID:12356864
Notes: GTU germline heterokaryon, progeny from CU428 x B 2086 transformed with p∆GNII construct, can mate with CU428, same mating type as CU427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01368 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1]
(?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Source References: PMID:16908532
Notes: Germline double KO heterokaryon of HHT3(by neo2 cassette) and HHT4(by blasticidin cassette). Non* side. Mates with 3N4B-KO-*
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02214 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: possible heterozygote BSR KO (bsr-r, mp-r)
Affected Genes: MLH
Notes: Selected after germline shoot of mic LH-BSR construct. The genotype is to be confirmed after maturing and testing. Same as Mic LH BSR Gl KO -LH1, independent strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01406 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcl1[∆?::neo3]/dcl1[∆?::neo3] (dcl1[∆?::neo3]/dcl1[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), DCL1 (TTHERM_00284230)
Genomic Alteration: Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Source References: PMID:16738316, PMID:15598983
Notes: ∆DCL1 homokaryon transformed with Bsr-MTT1-CNA1-GFP.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01806 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (chx1-1; cy-r, ory-r, MIX)
Genomic Alteration: Micronucleus: btu1-1::btu1-1M350K/btu1-1::btu1-1M350K (homozygous) Macronucleus: btu1-1::btu1-1M350K (homozygous)
Proper citation: RRID:TSC_SD00280 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht2[∆::neo2]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: Neo2 cassettes KO HHT2
Source References: PMID:9343391, PMID:16908532
Notes: HHT2 double knockout, neo 2 cassette
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01370 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, II)
Affected Genes: HHT1 (TTHERM_00570560)
Genomic Alteration: Micronucleus: Neo KO of HHT1
Source References: PMID:15701804
Notes: The germline knockout heterokaryon of HHT1 gene with neo cassette completely replacing coding region. Three independent cell lines #1,#2,# 3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02620 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; PMR1[C3]; MAT3; cy-s, pm-s, ?)
Source References: PMID:8725229, PMID:8601476
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01253 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: N terminal HA tag in TWI 11 with neo3 homozygous (pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Macronucleus: N-terminal HA tag on Twi 11
Notes: Homozygous homokaryon HA -Twi 11. Mate with HA-Twi11 #1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02067 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (snf2[∆::neo3/]snf2[∆::neo3], VII)
Affected Genes: SNF2 (TTHERM_00388250)
Genomic Alteration: Macronucleus: Neo 3 into coding of SNF2 in B2086
Notes: SNF2 family member involved in a variety of process including transcription regulation, DNA repair, DNA recombination and chromatin unwinding. The gene expression showed up- regulated during conjugation.
Pairs began to separate, the percent of anlagen is much lower than that of WT.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02189 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-86; None., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-86
Source References: PMID:21562224
Notes: Alanine scanning allele rescue of cen1∆ - Cen1 D86A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03278 Copy
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