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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00055007
Source Database: WormBase (WB)
Affected Genes: WBGene00015651(ceh-53)
Genomic Alteration: WBGene00015651(ceh-53)
Availability: unknown
Source References: EMPTY
Synonyms: ceh-53(ot1066) IV.
Notes: Made_by: Burcu Gulez (Hobert Lab)|"ot1066 is an engineered deletion of the full ceh-53 locus. Reference: Vidal B, et al. Elife. 2022 Mar 24;11:e76003. doi: 10.7554/eLife.76003. PMID: 35324425"
Proper citation: RRID:WB-STRAIN:WBStrain00055007 Copy
http://www.wormbase.org/db/get?name=WBStrain00054953
Source Database: WormBase (WB)
Affected Genes: WBGene00001867(him-8)
Genomic Alteration: WBGene00001867(him-8)
Availability: unknown
Source References: EMPTY
Synonyms: jsSi1973 I; him-8(e1489) IV.
Notes: jsSi1973 [mosL + loxP + myo-2p::FRT::nls::mNG::myo-2 3' + <{rps-0p::HygR::unc-54 3'} + ori <{Amp} <{mex-5p::nls::Cre::tbb-2 3'} + FRT3 + mosR] I. Him. Reference: Nonet ML. Rapid generation of C. elegans single copy transgenes combining RMCE and drug selection. bioRxiv 2023.03.05.531207; doi: https:|"Made_by: Nonet Lab"
Proper citation: RRID:WB-STRAIN:WBStrain00054953 Copy
http://www.wormbase.org/db/get?name=WBStrain00054945
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: jsSi1962 I.
Notes: jsSi1962 [mosL::loxP::FRT + myo-2p::nls::CyOFP::let-858 3' + mex-5p::FLP::D5::glh-2 3' + FRT3 mosR] I. Reference: Nonet ML. Rapid generation of C. elegans single copy transgenes combining RMCE and drug selection. bioRxiv 2023.03.05.531207; doi: https:|"Made_by: Nonet Lab"
Proper citation: RRID:WB-STRAIN:WBStrain00054945 Copy
http://www.wormbase.org/db/get?name=WBStrain00054947
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: jsSi1978 V.
Notes: jsSi1978 [loxP::FRT + myo-2p::nls::cyOFP::let-858 3' + mex-5p::FLP::D5::glh-2 3' + FRT3] V. Reference: Nonet ML. Rapid generation of C. elegans single copy transgenes combining RMCE and drug selection. bioRxiv 2023.03.05.531207; doi: https:|"Made_by: Nonet Lab"
Proper citation: RRID:WB-STRAIN:WBStrain00054947 Copy
http://www.wormbase.org/db/get?name=WBStrain00054946
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: jsSi1971 I.
Notes: jsSi1971 [mosL::loxP + mec-4Sp::FRT::nls::cyOFP::myo-2 3' + mex-5p::FLP::D5::glh-2 3' + FRT3 mosR] I. Reference: Nonet ML. Rapid generation of C. elegans single copy transgenes combining RMCE and drug selection. bioRxiv 2023.03.05.531207; doi: https:|"Made_by: Nonet Lab"
Proper citation: RRID:WB-STRAIN:WBStrain00054946 Copy
http://www.wormbase.org/db/get?name=WBStrain00054940
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: jsSi1727 I.
Notes: jsSi1727 [mosL::loxP::myo-2p::FRT::nlsCyOFP::myo-2 3' + mex-5p::FLP D5::glh-2 3' FRT3::mosR] I. Single component rapid RMCE landing site on Chromosome I at jsTi1453. Created from jsTi1453 (and jsSi1710) by two rounds of RMCE. Unpublished as of 5-2-2022. See https:
Proper citation: RRID:WB-STRAIN:WBStrain00054940 Copy
http://www.wormbase.org/db/get?name=WBStrain00054943
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: jsSi1900 II.
Notes: jsSi1900 [loxP::cup-4p::FRT::cyOFP::tbb-2 3' + mex-5p::FLP::D5::glh-2 3' FRT3] II. Reference: Nonet ML. Rapid generation of C. elegans single copy transgenes combining RMCE and drug selection. bioRxiv 2023.03.05.531207; doi: https:|"Made_by: Nonet Lab"
Proper citation: RRID:WB-STRAIN:WBStrain00054943 Copy
http://www.wormbase.org/db/get?name=WBStrain00054942
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: jsSi1837 IV.
Notes: jsSi1837 [loxP::mec-4Sp::FRT::nlsCyOFP::myo-2 3' + mex-5p::FLP D5::glh-2 3' FRT3] IV. Single component rapid RMCE landing site on Chromosome IV adjacent to cxTi10882. Created from jsSi1669 (and jsIs1824) by two rounds of RMCE. Unpublished as of 5-2-2022. See https:
Proper citation: RRID:WB-STRAIN:WBStrain00054942 Copy
http://www.wormbase.org/db/get?name=WBStrain00054938
Source Database: WormBase (WB)
Affected Genes: WBGene00001079(dpy-20)
Genomic Alteration: WBGene00001079(dpy-20)
Availability: unknown
Source References: EMPTY
Synonyms: dpy-20(e1282) IV; pkIs1275.
Notes: Made_by: Plasterk lab|"pkIs1275 [gpc-2::GFP + dpy-20(+)]. Reporter construct includes 2.3 kbp of upstream sequence and most of the gpc-2 open reading frame. 2.5 kbp PCR fragment generated with primers gpc2-1 (TCTGCAGCACGACGATAATC, extended with a SphI site) and gpc2-2 (GTCGATTGGGTTCACAAGTG, extended with a BamHI site) into vector pPD95.77. Reference: Jansen G, et al. EMBO J. 2002 Mar 1;21(5):986-94."
Proper citation: RRID:WB-STRAIN:WBStrain00054938 Copy
http://www.wormbase.org/db/get?name=WBStrain00055058
Source Database: WormBase (WB)
Affected Genes: WBGene00001190(egl-23)|WBGene00001454(flp-11)
Genomic Alteration: WBGene00001190(egl-23), WBGene00001454(flp-11)
Availability: unknown
Source References: EMPTY
Synonyms: flp-11(syb1433[flp-11::SL2::egl-23(cDNA)(A383V)::linker::mKate2]) X.
Notes: egl-23 cDNA(A383V) was knocked into the endogenous locus of flp-11 to express a potassium channel in RIS that causes moderate inactivation of RIS. Reference: Busack I & Bringmann H. PLOS Genetics 19(3): e1010665. https:|"Made_by: Bringmann lab"
Proper citation: RRID:WB-STRAIN:WBStrain00055058 Copy
http://www.wormbase.org/db/get?name=WBStrain00055057
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: hsp-12.6(syb1364[hsp-12.6::mKate2]) IV.
Notes: Made_by: SunyBiotech|"mKate2 tag was inserted into the endogenous hsp-12.6 locus using CRISPR/Cas9. HSP-12.6::mKate2 expression most visible in the muscles. Reference: Koutsoumparis A, et al. Curr Biol. 2022 Apr 26;S0960-9822(22)00581-4. doi: 10.1016/j.cub.2022.04.012. PMID: 35504281"
Proper citation: RRID:WB-STRAIN:WBStrain00055057 Copy
http://www.wormbase.org/db/get?name=WBStrain00055054
Source Database: WormBase (WB)
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
Source References: EMPTY
Synonyms: pha-1(e2123) III; otEx8047.
Notes: Made_by: Daniel Merritt|"otEx8047 [pks-1p::TagRFP + pha-1(+)]. Maintain at 25C to select for array. TagRFP expression marks CAN neurons."
Proper citation: RRID:WB-STRAIN:WBStrain00055054 Copy
http://www.wormbase.org/db/get?name=WBStrain00055055
Source Database: WormBase (WB)
Affected Genes: WBGene00000464(ceh-44)|WBGene00006807(unc-75)
Genomic Alteration: WBGene00000464(ceh-44), WBGene00006807(unc-75)
Availability: unknown
Source References: EMPTY
Synonyms: unc-75(ot1351) I; ceh-44(ot1015[ceh-44::GFP]) III.
Notes: Made_by: Michael Cesar|"ot1351 is a CRISPR-engineered deletion of unc-75 gene rendering all 3 RNA recognition motifs null on unc-75; reduces pan-neuronal nuclear GFP expression fluorescence. GFP tag inserted at the C-terminus of the endogenous ceh-44 locus by CRISPR. Please contact Oliver Hobert prior to publishing work using this strain."
Proper citation: RRID:WB-STRAIN:WBStrain00055055 Copy
http://www.wormbase.org/db/get?name=WBStrain00055061
Source Database: WormBase (WB)
Affected Genes: WBGene00003386(mod-1)
Genomic Alteration: WBGene00003386(mod-1)
Availability: unknown
Source References: EMPTY
Synonyms: mod-1(syb1841[mod-1::T2A::mNeonGreen]) V.
Notes: Endogenous mod-1 locus tagged with mNeonGreen. Inclusion of the T2A self-cleaving peptide allows mNeonGreen406to be expressed as a cytosolic protein. Generated in N2 background. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https:|"Made_by: Steve Flavell"
Proper citation: RRID:WB-STRAIN:WBStrain00055061 Copy
http://www.wormbase.org/db/get?name=WBStrain00055046
Source Database: WormBase (WB)
Affected Genes: WBGene00005016(sqt-1)|WBGene00006537(tbb-2)|WBGene00194659(linc-1)
Genomic Alteration: WBGene00005016(sqt-1), WBGene00006537(tbb-2), WBGene00194659(linc-1)
Availability: unknown
Source References: EMPTY
Synonyms: linc-1(ot1249[linc-1p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) I.
Notes: Made_by: Sandeep Wontakal|"The Null Transcriptional Reporter (NuTR) cassette was used to remove the linc-1 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in the male somatic gonad."
Proper citation: RRID:WB-STRAIN:WBStrain00055046 Copy
http://www.wormbase.org/db/get?name=WBStrain00055049
Source Database: WormBase (WB)
Affected Genes: WBGene00011141(nlp-66)
Genomic Alteration: WBGene00011141(nlp-66)
Availability: unknown
Source References: EMPTY
Synonyms: otIs669 V; nlp-66(syb4403[nlp-66:SL2:GFP::H2B])X.
Notes: GFP tag inserted at the C-terminus of the endogenous nlp-66 locus by CRISPR. Allele generated by SUNY Biotech. See description of strain OH15262 for full description of otIs669 NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene (Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642). Reference: Reilly MB, et al. bioRxiv 2022.04.29.490095; doi: https:|"Made_by: Suny Biotech"
Proper citation: RRID:WB-STRAIN:WBStrain00055049 Copy
http://www.wormbase.org/db/get?name=WBStrain00055042
Source Database: WormBase (WB)
Affected Genes: WBGene00004245(puf-9)
Genomic Alteration: WBGene00004245(puf-9)
Availability: unknown
Source References: EMPTY
Synonyms: puf-9(ot1236[puf-9::GFP::loxP::3xFLAG]) X.
Notes: Made_by: Sandeep Wontakal|"SEC cassette was used to generate a C-terminal GFP tag of puf-9. Expression is cytoplasmic and pan-somatic throughout larval development into adults."
Proper citation: RRID:WB-STRAIN:WBStrain00055042 Copy
http://www.wormbase.org/db/get?name=WBStrain00055050
Source Database: WormBase (WB)
Affected Genes: WBGene00006652(ttx-1)
Genomic Alteration: WBGene00006652(ttx-1)
Availability: unknown
Source References: EMPTY
Synonyms: ttx-1(syb1679[ttx-1::GFP]) ot1264) V.
Notes: Made_by: Berta Vidal (Hobert lab)|"ot1264 is a CRISPR deletion removing -10.8 kb to -1.8 kb before the first exon of ttx-1, made in the context of the ttx-1::GFP allele syb1679. Notably ttx-1 expression in RIB is lost, and RIB markers are off or dim. Reference: Reilly MB, et al. Widespread employment of conserved C. elegans homeobox genes in neuronal identity specification. bioRxiv 2022.04.29.490095; doi: https:"
Proper citation: RRID:WB-STRAIN:WBStrain00055050 Copy
http://www.wormbase.org/db/get?name=WBStrain00055052
Source Database: WormBase (WB)
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
Source References: EMPTY
Synonyms: pha-1(e2123) III; otIs355; otEx7950.
Notes: Made_by: Wendy Cao|"otIs355 [rab-3p(prom1)::2xNLS::TagRFP] IV. otEx7950 [clec-166::GFP + pha-1(+)]. Maintain at 25C to select for animals carrying the array. Pan-neuronal nuclear RFP expression. Co-expression of GFP and TagRFP in M5 neuron can be used to isolate M5 by FACS. Used by CeNGEN project for RNA-Seq (https:"
Proper citation: RRID:WB-STRAIN:WBStrain00055052 Copy
http://www.wormbase.org/db/get?name=WBStrain00055039
Source Database: WormBase (WB)
Affected Genes: WBGene00005016(sqt-1)|WBGene00006537(tbb-2)|WBGene00219694(linc-36)
Genomic Alteration: WBGene00005016(sqt-1), WBGene00006537(tbb-2), WBGene00219694(linc-36)
Availability: unknown
Source References: EMPTY
Synonyms: linc-36(ot1229[linc-36p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) IV.
Notes: Made_by: Sandeep Wontakal|"The Null Transcriptional Reporter (NuTR) cassette was used to remove the linc-36 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in the sperm of both hermaphrodites and males."
Proper citation: RRID:WB-STRAIN:WBStrain00055039 Copy
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