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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ubc2[∆::neo2]/ubc2[∆::neo2]
Affected Genes: UBC2, RAD6 (TTHERM_00550720)
Genomic Alteration: Micronucleus: Neo2 KO of UBC2
Source References: PMID:19822522
Notes: UBC2 KO homozygous heterokaryon. Mates with ∆UBC2gl 1D1.4, 1D1.8 and 2B1.2.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01329 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[α-β hybrid tail, ATU-B]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with pBTU1-D5 (D-DDDD) and Chimeric ATU1 gene, ATU-B (α-β hybrid ))
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D5 with (E437-442D). Some ATU1 replaced by ATU1 with chimeric α-β hybrid tail.
Source References: PMID:11864572, PMID:15254268, PMID:10831613
Notes: Progeny of DB6B x DB2A, co- transformed by constructs pBTU1-D5 (D-DDDD) and Chimeric ATU1 gene, pATU-B (α-β hybrid). See publications
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01848 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Notes: Submitted by P. Huvos, Southern Illinois Univesity
Proper citation: RRID:TSC_SD01735 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT2; cy-r, VII)
Source References: PMID:10880474
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01979 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 2R (CHX1; pmr1-1; cy-s, pm-r, II)
Notes: Deletion on right arm of chromosome 2.
Proper citation: RRID:TSC_SD00923 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2] (htax[∆::neo2]/htax[K123R]; hta1[∆::neo2]; mp-r, ?)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: Neo2 KO of HTAX and HTA1 Macronucleus: Neo2 KO of HTA1, partial KO of HTAX some HTAX has K123R mutation
Notes: New batch, rescue of h∆∆HTA-A1F14A1 and B1G6A. Not mating type II, VI, or VII.
This strain was marked as pm-r, but no longer is.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02399 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dbl1-1/dbl1-1 (dbl1-1, II)
Affected Genes: DBL1 (doublet-former 1)
Source References: PMID:18658256
Notes: A variable proclivity to form regular homopolar doublets. Enigmatic. Derivation highly complex, crosses of presumed homozygotes are inviable. Highly variable penetrance of doublets. It is not known how the doublets are actually formed.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01464 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 1 [chx1-1/chx1-1[B] or CHX1/CHX1[C3]] (cnj3, pm-r, VII)
Proper citation: RRID:TSC_SD00378 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445A,E446A]; pm-r, mp-?, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445A, E446A and neo replacing coding of some ATU1 genes
Source References: PMID:9037049, PMID:10831613
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pA5A6 derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E445A,E446A. No polyglycylation in this strain
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02558 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/gal1-1; tyrA5/tyrA5 (gal1-1; tyrA5; gal-r; tyr auxotroph, II)
Source References: PMID:6950403
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01502 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hhf1[∆::neo]/hhf1[∆::neo] (mpr1-1; mp-r, ?)
Affected Genes: HHT1 (TTHERM_00570560),HHT2 (TTHERM_00189180), HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo into HHT1, HHF1, HHT2/HHF2
Source References: PMID:16908532
Notes: These are intermediate germline KO heterokaryons. May be early batch and not tested for somatic rescue. Final germline KO strains are #4 and #7. Non-star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02039 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (rnh2[∆::neo3]; pm-r, VI)
Affected Genes: RNH2 (TTHERM_00142290)
Genomic Alteration: Macronucleus: neo3 KO of RNH2
Notes: Somatic KO of RNH2 (RNase H-2) of CU427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02831 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (rnh2[∆::neo3]; pm-r, VII)
Affected Genes: RNH2 (TTHERM_00142290)
Genomic Alteration: Macronucleus: neo3 KO of RNH2
Notes: Somatic KO of RNH2 (RNase H-2) of CU428
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02716 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding
Source References: PMID:10831613
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01906 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Notes: Collected by Paul Doerder on 7/29/2008 from the pond designated SG29 in PA (latitude 41.73, longitude -79.23)
Has a micronucleus. Mated with < 6 testers.
Proper citation: RRID:TSC_SD03091 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, D124A,E126A, HAc)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
Source References: PMID:12356864, PMID:16344310
Notes: pDE124/6 HA rescued GTUKO 5x6 progeny having mutation @ D124-A and E126-A in GTU1-1 gene, also one HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02044 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, I)
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00028 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, VI)
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00033 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr10-1[∆::NEO2] (sfr10-1[∆::NEO2], sfr8-1[∆::MTT-CHX]; Basal body organization defects., ?)
Affected Genes: SFR10 (TTHERM_00392670), SFR8 (TTHERM_00059100)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2] Macronucleus: sfr8-1[∆::MTT-CHX]
Notes: sfr10 complete KO with sfr8::MTT-CHX fully assorted.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03302 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (mlh1[243::neo2]; pm-r, ?)
Affected Genes: MLH1 (TTHERM_00471820)
Genomic Alteration: Macronucleus: Neo2 cassette inserted into coding of MLH1
Source References: PMID:7606784
Notes: Transformation of CU428xB2086, complete knockout of mic histone linker gene with neo2 cassette inserted into Stu1 site. Paromomycin res. to 2 mg/ml Cell lines LH3 and LH6 can mate with each other.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02694 Copy
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