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https://www.thermofisher.com/order/catalog/product/4376600
Equipment and software system that is a real-time thermal cycler for intuitive data analysis of polymerase chain reactions. It comes with analysis software, instrument control software, and a LED-based 4-color optical recording feature.
Proper citation: Thermo Fisher: StepOnePlus Real-Time PCR System (RRID:SCR_015805) Copy
http://ced.co.uk/products/power3
Data acquisition interface from Cambridge Electronic Design Limited. Works with Spike2 to provide continuous acquisition tasks. Records waveform data, digital and marker information, and can generate waveform and digital outputs simultaneously for real-time, multi-tasking experiment control.
Proper citation: CED: Power1401 (RRID:SCR_016040) Copy
Hardware that is a electrical pulse generator.
Proper citation: Grass Stimulator Model S88 (RRID:SCR_016192) Copy
http://www.localite.de/en/products/tms-navigator/
An instrument. Navigation system for transcranial magnetic stimulation (TMS).
Proper citation: Localite TMS Navigator (RRID:SCR_016126) Copy
http://www3.gehealthcare.com/en/products/categories/ep_recording/cardio_lab_recording_systems
Prucka CardioLab system delivers the EP data and visualization we need for electrophysiological study and catheter ablation.
Proper citation: Prucka CardioLab System (RRID:SCR_016180) Copy
http://edboyden.org/05.09.boyden.html
Laser tool that enables neurons to be optically silenced by pulses of yellow light, the light-activated chloride pump halorhodopsin (Halo), in a paper entitled Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers. The quest to determine how precise neural activity patterns mediate computation, behavior, and pathology would be greatly aided by a set of tools for reliably activating and inactivating genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having earlier adapted a light-activated cation channel, 1channelrhodopsin-2 (ChR2), for allowing neurons to be stimulated by blue light, we searched for a complementary tool that would enable optical neuronal inhibition, driven by light of a second color. Here we report that targeting the 1codon-optimized form of the light-driven chloride pump halorhodopsin from the archaebacterium Natronomas pharaonis (hereafter abbreviated Halo) to genetically-specified neurons enables them to be silenced reliably, and reversibly, by millisecond-timescale pulses of yellow light. We show that trains of yellow and blue light pulses can drive high-fidelity sequences of hyperpolarizations and depolarizations in neurons simultaneously expressing yellow light-driven Halo and blue light-driven ChR2, allowing for the first time manipulations of neural synchrony without perturbation of other parameters such as spiking rates. The Halo/ChR2 system thus constitutes a powerful toolbox for multichannel photoinhibition and photostimulation of virally or transgenically targeted neural circuits without need for exogenous chemicals, enabling systematic analysis and engineering of the brain, and quantitative bioengineering of excitable cells.
Proper citation: Channelrhodopsin-2 enables optical activation of neurons (RRID:SCR_008833) Copy
Core laboratory for nucleic acid sequencing and bioinformatics. Used for research support, education, and training. Services include genomic techniques and applications, sequencing technologies, and bioinformatics analyses, writting letters of support for grant applications submitted to funding agencies. GGBC operates multiple platforms for short-, long-, and single-molecule sequencing reads (i.e., Illumina MiSeq and NextSeq, PacBio Sequel, and Oxford Nanopore MinIon).
Proper citation: Georgia Genomics and Bioinformatics Core at the University of Georgia (RRID:SCR_010994) Copy
Benchtop genetic analyzer. Electrophoresis system for DNA and RNA sample quality control. It can be used for next generation sequencing or Biobank workflow.
Proper citation: Agilent: 4200 TapeStation System (RRID:SCR_018435) Copy
http://tools.thermofisher.com/content/sfs/manuals/cms_040970.pdf
Automated PCR instrument for automated amplification of nucleic acids with Polymerase Chain Reaction. It has reaction volumes of up to 50 uL and sample temperature range of 4 to 99.9 C.
Proper citation: Thermo Fisher: Applied Biosystems: GeneAmp 9700 PCR Thermocycler System (RRID:SCR_018436) Copy
https://www.rankinbiomed.com/product/refurbished-american-optical-860-sliding-micrtome/
THIS REOURCE IS NO LONGER IN SERVICE. Documented on September 29,2023. Sliding microtome used for cutting hard objects such as bone, wood, frozen celloidin, and paraffin preparations. Cutting thickness is adjustable from 1 to 40 microns.
Proper citation: American Optical: 860 Sliding Microtome (RRID:SCR_018434) Copy
https://atagousa.corecommerce.com/PAL-10S-p358.html
Digital hand held pocket refractometer that measures urine specific gravity. It has measurement temperature of 10 to 35 C and sample volume of 0.3ml.
Proper citation: Atago: PAL-10S Pocket Refractometer (RRID:SCR_018439) Copy
https://www.sutter.com/manuals/P-2000_OpMan.pdf
Microprocessor controlled, CO2 laser based micropipette puller.Default configuration allows fabrication of micropipettes for intracellular recording, patch clamping, microinjection and microperfusion.Can store up to 100 separate programs, each consisting of up to 8 command lines. The five parameters set in each command line allow exquisite control of the micropipette taper geometry. Allows the use of wide range of glass diameters from 0.125 to 1.2 mm.
Proper citation: Sutter: P-2000 Laser Micropipette Puller (RRID:SCR_018640) Copy
https://github.com/PIEFoundry/Microfabrication/wiki/Parylene-MEA-Fabrication
Parylene MEA Fabrication is related to SPARC. Repository for standard fabrication procedure for Parylene C microelectrode arrays and similar devices.
Proper citation: HORNET CENTER FOR AUTONOMIC NERVE RECORDING AND STIMULATION SYSTEMS (RRID:SCR_023681) Copy
https://www.nanostring.com/products/ncounter-analysis-system/ncounter-systems-overview/
System includes hardware and software. Options include nCounter SPRINT,nCounter MAX or nCounter FLEX. Platform used for multiplex analysis of up to 800 RNA, DNA, or protein targets. System can be combined with GeoMx Digital Spatial Profiler (DSP) to enable high-plex, spatially-resolved RNA, and protein quantification.
Proper citation: NanoString: nCounter Analysis System (RRID:SCR_021712) Copy
https://www.cellscale.com/products/microtester/
System includes MicroTester G2 device and software. MicroTester G2 is mechanical test device that can be used to test properties of variety of tissues and materials. Main components of device are load application microbeam, actuators, imaging system, and fluid bath/test chamber. MicroTester G2 software is divided into two modules: data collection module and review and analysis module.
Proper citation: CellScale: MicroTester G2 system (RRID:SCR_022034) Copy
https://www.coolled.com/products/pe-300ultra/
Highly controllable fluorescence microscopy Illumination System which offers broad spectrum LED illumination for imaging most common fluorophores. System offers precise control over wavelength irradiance and shuttering. Used for LED microscope lighting.
Proper citation: CoolLED: pE-300ultra (RRID:SCR_021972) Copy
https://www.alveolelab.com/our-products/primo-micropatterning/
Photopatterning device for performing micropatterning, microfabrication and hydrogel polymerization.Part of PRIMO system.
Proper citation: Alveole: PRIMO photopatterning module (RRID:SCR_021941) Copy
https://biomedical-instruments.com/pdf/Narishige%20PC-10.pdf
Vertical pipette puller to create microelectrodes from borosilicate capillaries.
Proper citation: Narishige: PC 10 Puller (RRID:SCR_022057) Copy
Horizontal pipette puller to pull and polish microelectrodes from borosilicate capillaries.
Proper citation: Zeitz: DMZ Universal Electrode Puller (RRID:SCR_022056) Copy
https://biomedical-instruments.com/pdf/Narishige%20MF-830.pdf
Powerful microscope mounted to produce microelectrodes for patch clamping.
Proper citation: Narishige: MF 830 Microgrinder (RRID:SCR_022055) Copy
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