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Species: Arabidopsis thaliana
Genetic Insert: promoter, RbcS3B (AT5g38410, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50260 Copy
Species: Arabidopsis thaliana
Genetic Insert: UBQ10 promoter
Vector Backbone Description: Backbone Marker:Mark Curtis at ETH Zurich (Brand et al., Plant Physiology 2006); Vector Backbone:pLB12; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Comments: The vector pLB12 contains both an activator unit and a b-glucuronidase (GUS) reporter within a responder unit.
Proper citation: RRID:Addgene_53449 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100281 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Proper citation: RRID:Addgene_100537 Copy
Species: Arabidopsis thaliana
Genetic Insert: OEP7(1–50)-Citrine
Vector Backbone Description: Vector Backbone:pMpGWB106; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28634853
Proper citation: RRID:Addgene_100602 Copy
Species: Arabidopsis thaliana
Genetic Insert: acd11
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24412362
Proper citation: RRID:Addgene_170738 Copy
Species: Arabidopsis thaliana
Genetic Insert: ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Donor vector / entry clone; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34059917
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_170723 Copy
Species: Arabidopsis thaliana
Genetic Insert: ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Donor vector / entry clone; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34059917
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_170725 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtACT2t
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34520169
Comments: Insert can be excised by BsaI digestion . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170882 Copy
Species: Arabidopsis thaliana
Genetic Insert: GID1-VPR-IRES-HygR
Vector Backbone Description: Vector Backbone:pSLQ2842 (Addgene #84244); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34133940
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_170808 Copy
Species: Arabidopsis thaliana
Genetic Insert: PIF6APB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4860; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22276 Copy
Species: Arabidopsis thaliana
Genetic Insert: IntersectinDHPH-20aaLinker-mYFP-PIF6APB
Vector Backbone Description: Backbone Size:4860; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22278 Copy
Species: Arabidopsis thaliana
Genetic Insert: p!SV40-PakGBD-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4864; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22280 Copy
Species: Arabidopsis thaliana
Genetic Insert: p!SV40-mCherry-WaspGBD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5216; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22281 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G72180
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: STVEKQALFRFKNRLDDSHNILQSWKPSDSPCVFRGITCDPLSGEVIGISLGNVNLSGTISPSISALTKLSTLSLPSNFISGRIPPEIVNCKNLKVLNLTSNRLSGTIPNLSPLKSLEILDISGNFLNGEFQSWIGNMNQLVSLGLGNNHYEEGIIPESIGGLKKLTWLFLARSNLTGKIPNSIFDLNALDTFDIANNAISDDFPILISRLVNLTKIELFNNSLTGKIPPEIKNLTRLREFDISSNQLSGVLPEELGVLKELRVFHCHENNFTGEFPSGFGDLSHLTSLSIYRNNFSGEFPVNIGRFSPLDTVDISENEFTGPFPRFLCQNKKLQFLLALQNEFSGEIPRSYGECKSLLRLRINNNRLSGQVVEGFWSLPLAKMIDLSDNELTGEVSPQIGLSTELSQLILQNNRFSGKIPRELGRLTNIERIYLSNNNLSGEIPMEVGDLKELSSLHLENNSLTGFIPKELKNCVKLVDLNLAKNFLTGEIPNSLSQIASLNSLDFSGNRLTGEIPASLVKLKLSFIDLSGNQLSGRIPPDLLAVGGSTAFSRNEKLCVDKENAKTNQNLGLSICSGYQNVKRNS
Proper citation: RRID:Addgene_114899 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G07560
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: QGFISLDCGLQADESPYTEPLTKLTFTSDADFIKSGKSGKIQNVPGMEYIKPYTVLRYFPDGVRNCYTLIVIQGTNYLIVAMFTYGNYDNLNTHPKFDLYLGPNIWTTVDLQRNVNGTRAEIIHIPRSTSLQICLVKTGTTTPLISALELRPLRNNTYIPQSGSLKTLFRVHLTDSKETVRYPEDVHDRLWSPFFMPEWRLLRTSLTVNTSDDNGYDIPEDVVVTAATPANVSSPLTISWNLETPDDLVYAYLHVAEIQSLRENDTREFNISAGQDVNYGPVSPDEFLVGTLFNTSPVKCEGGTCHLQLIKTPKSTLPPLLNAIEAFITVEFPQSETNANDVLAIKSIETSYGLSRISWQGDPCVPQQLLWDGLTCEYTNMSTPPRIHSLDLSSSELTGIIVPEIQNLTELKKLDFSNNNLTGGVPEFLAKMKSLLVINLSGNNLSGSVPQALLNKVKNGLKLNIQGNPNLCFSSSCNKKK
Proper citation: RRID:Addgene_114939 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G07550
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SLDCGLASNESPYNEANSNLTYISDADFIQGGKTGNVQKDLLMKLRKPYTVLRYFPDGIRNCYSLNVKQDTNYLIRVMFRYGNYDGLNNSPRFDLYLGPNIWTTIDMGKSGDGVLEEIIHITRSNILDICLVKTGTSTPMISSIELRPLLYDTYIAQTGSLRNYNRFYFTDSNNYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTITWNLKTATDQVYGYIYIAEIMEVQANETREFEVVVNNKVHFDPFRPTRFEAQVMFNNVPLTCEGGFCRLQLIKTPKSTLPPLMNAFEIFTGIEFPQSETNQNDVIAVKNIQASYGLNRISWQGDPCVPKQFLWTGLSCNVIDVSTPPRIVKLDLSSSGLNGVIPPSIQNLTQLQELDLSQNNLTGKVPEFLAKMKYLLVINLSGNKLSGLVPQALLDRKKEGLKLLVDENMICVSCGT
Proper citation: RRID:Addgene_114938 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G05700
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SGFISIDCGIPSGSSYKDDTTGINYVSDSSFVETGVSKSIPFTAQRQLQNLRSFPEGSRNCYTLIPIQGKGKKYLIRASFMYGNYDGENGSPEFDLFLGGNIWDTVLLSNGSSIVSKEVVYLSQSENIFVCLGNKGKGTPFISTLELRFLGNDNTTYDSPNGALFFSRRWDLRSLMGSPVRYDDDVYDRIWIPRNFGYCREINTSLPVTSDNNSYSLSSLVMSTAMTPINTTRPITMTLENSDPNVRYFVYMHFAEVEDLSLKPNQTREFDISINGVTVAAGFSPKYLQTNTFFLNPESQSKIAFSLVRTPKSTLPPIVNALEIYVANSFSQSLTNQEDGDAVTSLKTSYKVKKNWHGDPCLPNDYIWEGLNCSYDSLTPPRITSLNLSSSGLTGHISSSFSNLTMIQELDLSNNGLTGDIPEFLSKLKFLRVLNLENNTLTGSVPSELLERSNTGSFSLRLGENPGLCTEISCRKSNS
Proper citation: RRID:Addgene_114937 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G51800
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SGFISLDCGSPRETSFREKTTNITYISDANFINTGVGGSIKQGYRTQFQQQTWNLRSFPQGIRNCYTLNLTIGDEYLIRANFLHGGYDDKPSTQFELYLGPNLWSTVTTTNETEASIFEMIHILTTDRLQICLVKTGNATPFISALELRKLMNTTYLTRQGSLQTFIRADVGATVNQGYRYGIDVFDRVWTPYNFGNWSQISTNQSVNINNDYQPPEIAMVTASVPTDPDAAMNISLVGVERTVQFYVFMHFAEIQELKSNDTREFNIMYNNKHIYGPFRPLNFTTSSVFTPTEVVADANGQYIFSLQRTGNSTLPPLLNAMEIYSVNLLPQQETDRKEVDAMMNIKSAYGVNKIDWEGDPCVPLDYKWSGVNCTYVDNETPKIISLDLSTSGLTGEILEFISDLTSLEVLDLSNNSLTGSVPEFLANMETLKLINLSGNELNGSIPATLLDKERRGSITLSIEGNTGLCSSTSCATTKKK
Proper citation: RRID:Addgene_114936 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G46330
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: KQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKR
Proper citation: RRID:Addgene_114920 Copy
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