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Vector Backbone Description: Backbone Size:17653; Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30240613
Comments: Selectable Marker: Tetracycline; 30 μg/mL in E.coli and 100 μg/mL in Pseudomonas aeruginosa
Proper citation: RRID:Addgene_113347 Copy
Genetic Insert: sacB
Vector Backbone Description: Vector Backbone:pDAI-SceI; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25172289
Comments: Addgene NGS found K43E and T318P within the SacB translation compared to the best match NCBI reference sequence (ACF93714.1). The depositing laboratory confirms that these mutations should not adversely affect plasmid function
Proper citation: RRID:Addgene_113635 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111252 Copy
Species: Synthetic
Genetic Insert: TorA signal peptide
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111253 Copy
Species: Synthetic
Genetic Insert: DsRed-Express2
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111251 Copy
Species: Synthetic
Genetic Insert: DsRed-Express2
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111257 Copy
Species: Synthetic
Genetic Insert: TorA(sp)-mTurquoise2
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111254 Copy
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111249 Copy
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111248 Copy
Genetic Insert: F' Tra operon
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16141338
Comments: F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.
Proper citation: RRID:Addgene_12082 Copy
Vector Backbone Description: Vector Backbone:p6000; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:18944940
Proper citation: RRID:Addgene_113659 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649424 , Alternative ID = pGT403 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114164 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649422, Alternative ID = pGT401 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114162 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649425 , Alternative ID = pGT406 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114167 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649426, Alternative ID = pGT405 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114166 Copy
Genetic Insert: sox3gRNAT2
Vector Backbone Description: Vector Backbone:pU6; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127778 Copy
Genetic Insert: sox3gRNAT1
Vector Backbone Description: Vector Backbone:pU6; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127777 Copy
Species: Streptococcus thermophilus
Genetic Insert: dcas9 (Streptococcus thermophilus #3)
Vector Backbone Description: Backbone Size:5431; Vector Backbone:pVCERC-5; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31937638
Proper citation: RRID:Addgene_133319 Copy
Species: Salmonella typhimurium ATCC14028 Nal resistant
Genetic Insert: adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
Vector Backbone Description: Backbone Marker:Guzman et al, J Bacteriol, 1995, 177(14):4121-30; Backbone Size:4919; Vector Backbone:pBAD30; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:15306016
Proper citation: RRID:Addgene_126650 Copy
Species: Enterobacteria phage lambda
Genetic Insert: cI
Vector Backbone Description: Vector Backbone:pFG036; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32963323
Comments: This plasmid was made to repress the expression of the transposase (pL promoter) in pFG051.
This plasmid has to be in the bacterium to avoid transposition of pFG051's transposon.
It has to be in the donor strain for conjugation to avoid unwanted transposition in the donor.
A strain carrying this plasmid was used to build pFG051.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_137996 Copy
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