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Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74800 Copy
Species: Synthetic
Genetic Insert: RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG
Vector Backbone Description: Backbone Marker:VIB, Ghent Univ, Karimi et al. Trends Plant Sci. (2002); Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:33098708
Comments: Gateway destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter. Use with Platinum Gate TALEN kit (Kit # 1000000043) and plasmids #158728-#158735.
Proper citation: RRID:Addgene_163145 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141301 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141302 Copy
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:13299; Vector Backbone:pRATIO1212; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141311 Copy
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:14000; Vector Backbone:pRATIO2214; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141314 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10844; Vector Backbone:pGWB401; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141285 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11165; Vector Backbone:pGWB501; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141286 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10700; Vector Backbone:pGWB601; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141287 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141299 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141296 Copy
Vector Backbone Description: Backbone Size:9506; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35250 Copy
Vector Backbone Description: Backbone Size:7773; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35253 Copy
Vector Backbone Description: Backbone Size:9298; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35247 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54361 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54360 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54365 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54363 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54362 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-S (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54346 Copy
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