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Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Hodaka Fujii; Backbone Size:6238; Vector Backbone:pMXs-neo; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051498
Comments: The coding sequence of 3xFLAG-dCas9 can be cleaved with Pac I and Not I.
Construction strategy of gRNA retroviral vectors
1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I).
2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors.
Vectors & Sites of insertion
pSIR-neo (Addgene #51128): eg. [Xho I + Hind III]
pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I
pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I
pSIR-hCD2 (Addgene #51143): eg. EcoR I
Proper citation: RRID:Addgene_51260 Copy
Species: Synthetic
Genetic Insert: Pyronic
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5419; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24465702
Proper citation: RRID:Addgene_51308 Copy
Species: Synthetic
Genetic Insert: mNeptune2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5425; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633408
Proper citation: RRID:Addgene_51309 Copy
Species: Synthetic
Genetic Insert: mNeptune2.5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5425; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633408
Proper citation: RRID:Addgene_51310 Copy
Species: Synthetic
Genetic Insert: 2NQA
Vector Backbone Description: Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2NQA/
Proper citation: RRID:Addgene_51330 Copy
Species: Synthetic
Genetic Insert: SeqFosC
Vector Backbone Description: Backbone Size:7372; Vector Backbone:pAF1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24981864
Proper citation: RRID:Addgene_51428 Copy
Species: Synthetic
Genetic Insert: SeqFosA
Vector Backbone Description: Backbone Size:7485; Vector Backbone:pAF1-VP64; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24981864
Proper citation: RRID:Addgene_51430 Copy
Species: Synthetic
Genetic Insert: SeqFosC
Vector Backbone Description: Backbone Size:7705; Vector Backbone:pAF1-VP16; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24981864
Proper citation: RRID:Addgene_51432 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Right (R18) Can C-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51439 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Right (R18) Q3 C-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51441 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Left (L18) Q3 C-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51440 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Right (R18) N3 N-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51449 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Left (L18) N3 N-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51448 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Left (L18) N1 N-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51446 Copy
Species: Synthetic
Genetic Insert: Replacing the cpc operon with kanamycin resistance
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4835; Vector Backbone:pJ207; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25046143
Comments: Formighieri C, Melis A (2015) A phycocyanin·phellandrene synthase fusion enhances recombinant protein expression and β-phellandrene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria). Metab Eng 32:116–124. http://dx.doi.org/10.1016/j.ymben.2015.09.010
Proper citation: RRID:Addgene_51468 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23893733
Comments: The sfGFP in this plasmid contains Y148C and K214I mutations that do not affect the function of the plasmid.
Proper citation: RRID:Addgene_51559 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51562 Copy
Species: Synthetic
Genetic Insert: Quasar2-mOrange-Chr64-GFP
Vector Backbone Description: Vector Backbone:lentivirus backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51656 Copy
Species: Synthetic
Genetic Insert: aDTX-lumitoxin
Vector Backbone Description: Backbone Size:5341; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24407101
Proper citation: RRID:Addgene_51686 Copy
Species: Synthetic
Genetic Insert: SpyLigase
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:6400; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24639550
Comments: SnoopLigase is the more recent peptide-peptide ligation technology from the Howarth lab and is now preferred over SpyLigase in terms of more general reaction conditions and higher coupling yield on a range of proteins.
pET28a-AviTag-SnoopLigase https://www.addgene.org/105626/
pET28a-HaloTag7-SnoopLigase https://www.addgene.org/105627/
SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Jacob O. Fierer, Gianluca Veggiani and Mark Howarth
Transform plasmid into BL21 DE3 RIPL for expression.
Proper citation: RRID:Addgene_51722 Copy
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