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Species: Arabidopsis thaliana
Genetic Insert: EC1.2en-EC1.1p fusion promoter
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105359 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor SAG101 (At5g14920)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105368 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor PAD4 (At3g52430)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105366 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor EDS1 (At3g48090)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105364 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor SAG101 (At5g14920)
Vector Backbone Description: Vector Backbone:pAGM1251 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105369 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B (1-621 aa)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB621-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB621-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100282 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome interacting factor 3
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PIF3-mEGFP in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
Proper citation: RRID:Addgene_100283 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Proper citation: RRID:Addgene_100538 Copy
Species: Arabidopsis thaliana
Genetic Insert: OEP7(1–50)
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28634853
Proper citation: RRID:Addgene_100601 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B (1-908 aa, Y276H mutation)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB-Y276H-mCherry-HRasCT in mammalian cells.
PhyB-Y276H has two additional missense mutations (P840S, P884L); the construct will function as described in the publication.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100540 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-LS-F138I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100922 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-GS-L255I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100966 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-GS
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100967 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-GS-F138I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100965 Copy
Species: Arabidopsis thaliana
Genetic Insert: nlsGPS1
Vector Backbone Description: Backbone Marker:PMID: 24576426; Vector Backbone:p16-Kan vector; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28970478
Proper citation: RRID:Addgene_101081 Copy
Species: Arabidopsis thaliana
Genetic Insert: ipi1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11004185
Proper citation: RRID:Addgene_53275 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyB
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9476; Vector Backbone:pAC-LYC; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17085635
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53288 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyE
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:11849; Vector Backbone:pAC-NEUR; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53286 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyE
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17634749
Proper citation: RRID:Addgene_53285 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac1;2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47765 Copy
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