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Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pME-FLAG-CD59-GPI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20477992
Proper citation: RRID:Addgene_50378 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pME-puro-FLAG-CD59; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20477992
Comments: Please click View Sequences link for full plasmid sequence of vector backbone pME-puro-FLAG-CD59.
Proper citation: RRID:Addgene_50377 Copy
Species: Synthetic
Genetic Insert: DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRS426 Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11000112
Comments: There is a H to T mutation in front of the MetBetaGal. The mutation should not affect function.
Proper citation: RRID:Addgene_50572 Copy
Species: Synthetic
Genetic Insert: zCas9D10A
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50582 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50580 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50587 Copy
Species: Synthetic
Genetic Insert: zCas9D10A
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50589 Copy
Species: Synthetic
Genetic Insert: T1, gRNA scaffold, OsU3t plus, TaU3p, T2
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Comments: Please note that this plasmid runs as a dimer (>7kb) or multimer.
Proper citation: RRID:Addgene_50593 Copy
Species: Synthetic
Genetic Insert: T3, gRNA scaffold, U6-26t plus, OsU3p, T4
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50595 Copy
Species: Synthetic
Genetic Insert: T1, U626t plus, U629p, T2
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50590 Copy
Species: Synthetic
Genetic Insert: T3, gRNA scaffold, U6-1tplus, U6-26p, T4
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50592 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Registry of Standard Biological Parts; Backbone Size:3296; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24608181
Comments: Note: Addgene's quality control sequencing found a few mismatches compared to the depositor's full sequence, but they should not affect plasmid function.
Proper citation: RRID:Addgene_50549 Copy
Species: Synthetic
Genetic Insert: Cph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter.
Requires pPLPCB(S)t or some other source of TetR
Requires PCB to function
Does NOT include the response regulator.
Proper citation: RRID:Addgene_50552 Copy
Species: Synthetic
Genetic Insert: Luciferase-let7-Poly(A)
Vector Backbone Description: Backbone Marker:The Drosophila Gateway™ Vector Collection; Vector Backbone:pAHW; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23123195
Comments: This plasmid expresses a Renilla luciferase reporter for let-7 miRNA with a regular polyadenylation signal.
Proper citation: RRID:Addgene_50556 Copy
Species: Synthetic
Genetic Insert: 3xGFP
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12566428
Comments: Please note that this plasmid is unstable due to the tandem yeGFP genes. Screening of colonies is required prior to storage. See diagnostic digest for expected results.
Proper citation: RRID:Addgene_50659 Copy
Species: Synthetic
Genetic Insert: split EGFP
Vector Backbone Description: Backbone Size:5138; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24284873
Proper citation: RRID:Addgene_50716 Copy
Species: Synthetic
Genetic Insert: ddRFP A and ddRFP B
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Comments: There is a small discrepancy between the reference sequence and Addgene's quality control sequence, this causes an A to G mutation in the linker region between NLS and ddRFP B. This change should not affect plasmid function.
Proper citation: RRID:Addgene_50840 Copy
Species: Synthetic
Genetic Insert: ddGFP A and ddGFP B
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Proper citation: RRID:Addgene_50842 Copy
Species: Synthetic
Genetic Insert: ddRFP A and ddRFP B
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Proper citation: RRID:Addgene_50849 Copy
Species: Synthetic
Genetic Insert: ddGFP A
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Proper citation: RRID:Addgene_50852 Copy
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