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Species: Synthetic
Genetic Insert: hChR2(E123T/H134R)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Penn Vector Core PV2355
Proper citation: RRID:Addgene_100050 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Penn Vector Core PV1959
Proper citation: RRID:Addgene_100054 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: ChR2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Penn Vector Core PV1522
Proper citation: RRID:Addgene_100056 Copy
Species: Homo sapiens
Genetic Insert: AURKB
Vector Backbone Description: Backbone Size:7800; Vector Backbone:PHAGE-P-CMVt-N-HA-GAW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25476789
Proper citation: RRID:Addgene_100142 Copy
Species: Homo sapiens
Genetic Insert: CPT1A
Vector Backbone Description: Backbone Size:7800; Vector Backbone:PHAGE-P-CMVt-N-HA-GAW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25476789
Proper citation: RRID:Addgene_100146 Copy
Genetic Insert: sfGCaMP6s-T78H
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:2973; Vector Backbone:pRSET A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100023 Copy
Species: Synthetic
Genetic Insert: Antares2
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28869756
Comments: Please see https://med.virginia.edu/ai-lab/samples/ for Practical Notes describing the use of this plasmid.
Proper citation: RRID:Addgene_100027 Copy
Species: Thermosynechococcus elongatus bp-1 for PcyA and HO1, and Synechocystis sp. PCC 6803 for Fd and Fnr.
Genetic Insert: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to human BVRA is expressed so that PCB is further accumulated.
Proper citation: RRID:Addgene_100285 Copy
Species: Homo sapiens
Genetic Insert: 18 bp RFLP (MluI+KspAI)
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Human Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Proper citation: RRID:Addgene_100292 Copy
Species: Homo sapiens
Genetic Insert: 18 bp RFLP (MluI+KspAI)
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Human Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al.) for homology-directed AAVS1 targeting.
Proper citation: RRID:Addgene_100290 Copy
Species: Homo sapiens
Genetic Insert: PuroR.T2A.EGFP expression unit
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al. 2017) for inducing homology-directed AAVS1 targeting. This donor plasmid has its targeting module flanked by the target site (TS) for gRNA.S1: 5’-CTGTCCCCTCCACCCCACAGTggggccactagggacaggattggTGACAGA-3’.
Proper citation: RRID:Addgene_100297 Copy
Species: Homo sapiens
Genetic Insert: PuroR.T2A.EGFP expression unit
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al.) for homology-directed AAVS1 targeting.
Proper citation: RRID:Addgene_100296 Copy
Species: Homo sapiens
Genetic Insert: FTL
Vector Backbone Description: Backbone Size:7800; Vector Backbone:PHAGE-P-CMVt-N-HA-GAW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25476789
Proper citation: RRID:Addgene_100153 Copy
Species: Mus musculus
Genetic Insert: Kdm6b
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28723896
Proper citation: RRID:Addgene_100278 Copy
Vector Backbone Description: Vector Backbone:pLKO_TRC005; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28805815
Proper citation: RRID:Addgene_100277 Copy
Species: Other
Genetic Insert: LF82_782
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100385 Copy
Species: Homo sapiens
Genetic Insert: MAT2A
Vector Backbone Description: Backbone Marker:Carson Thoreen at Yale University; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100521 Copy
Species: X. dendrorhous
Genetic Insert: GGPP synthase
Vector Backbone Description: Backbone Size:4846; Vector Backbone:pLM304; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Comments: Addgene NGS sequencing found the following mutations compared to the provided reference sequence: crtE with M176I, K346R, I347M, E353D mutations, crtI with L107F, H427Q mutations, and crtYB with A190T, E307K mutations. tHMG1 contains only residues 531-1054 compared to the NCBI reference seq [M22002.1]. These mutations/truncation do not affect plasmid function.
Proper citation: RRID:Addgene_100539 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Proper citation: RRID:Addgene_100537 Copy
Species: Rattus norvegicus
Genetic Insert: S6K1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100509 Copy
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