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Species: Synthetic
Genetic Insert: T2A-td-sfGFP-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182339 Copy
Species: Synthetic
Genetic Insert: ArcLight-ST
Vector Backbone Description: Backbone Size:4831; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34893595
Proper citation: RRID:Addgene_182318 Copy
Species: Synthetic
Genetic Insert: Kv-ArcLight-ST
Vector Backbone Description: Backbone Size:4794; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34893595
Proper citation: RRID:Addgene_182319 Copy
Species: Synthetic
Genetic Insert: 4x miR-124 target sites
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Four copies of miR-124 target sites (Åkerblom et al., 2012, https://doi.org/10.1523/JNEUROSCI.0558-12.2012) were cloned into “StLa” reporter plasmid (pCAG-loxP-pEM7-Neo*-bGHpA-3xSV40pA-loxP-tauLacZ-bGHpA from Nam and Benezra, 2009, https://doi.org/10.1016/j.stem.2009.08.017) without the tauLacZ insert and the Rosa26 homology arms. The 4x miR-124 target sequence can be released with BamHI and NotI.
More information on the “StLa” reporter at
(1) http://www.informatics.jax.org/allele/MGI:4366911
(2) https://figshare.com/ndownloader/files/38884434 (clicking this link will download a file)
Proper citation: RRID:Addgene_182363 Copy
Species: Synthetic
Genetic Insert: Myr-DeadCasp8-ERT2 and Myr-Casp8-ERT2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Synthetic Biology, dre / rox , FLP / FRT; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182360 Copy
Species: Synthetic
Genetic Insert: Floxed hChR2(H134R)-tdTomato
Vector Backbone Description: Backbone Size:15658; Vector Backbone:Rosa26 Targeting Vector; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22446880
Proper citation: RRID:Addgene_34630 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:2070; Vector Backbone:pPROLar.A122; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19915540
Comments: MG1655 for expression
Proper citation: RRID:Addgene_34617 Copy
Species: Synthetic
Genetic Insert: PCP-2x-yeGFP
Vector Backbone Description: Vector Backbone:pURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21512033
Comments: This plasmid expresses a chimeric RNA-binding protein fused to GFP used to detect mRNAs that contain multiple copies of an RNA sequence (see Addgene plasmid #'s 35191, 35192 & 35196). See associated publication for detailed methods and use of this plasmid.
Please note that Addgene's sequencing results identified discrepancies when compared to the assembled sequence provided by the depositing laboratory. The first GFP construct in this plasmid (bp#441-1154) is yeast codon optimized and contains a E124K mutation and amino acid 206 is A (not K, as suggested by the depositor's sequence). The second GFP construct in this plasmid (bp# 1212-1928) is NOT yeast codon optimized.
This construct has successfully been used by the depositing laboratory as detailed in the associated publication and the sequencing discrepancies are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_35194 Copy
Species: Synthetic
Genetic Insert: GLT1 POL1 24xPP7SL
Vector Backbone Description: Vector Backbone:pHIS; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21512033
Comments: Note that Addgene NGS results found 22xPP7 stem loop repeats rather than expected 24. It is not known how this impacts plasmid function.
This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.
Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.
Proper citation: RRID:Addgene_35196 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2777; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000090; Origin: colE1
Proper citation: RRID:Addgene_35324 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2836; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000088; Origin: colE1
Proper citation: RRID:Addgene_35322 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000087; Origin: SC101
Proper citation: RRID:Addgene_35321 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4245; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000094; Origin: SC101
Proper citation: RRID:Addgene_35328 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2764; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000093; Origin: p15A
Proper citation: RRID:Addgene_35327 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000092; Origin: p15A
Proper citation: RRID:Addgene_35326 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2823; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000091; Origin: p15A
Proper citation: RRID:Addgene_35325 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3637; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000048; Origin: p15A
Proper citation: RRID:Addgene_35282 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3489; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000053; Origin: colE1
Proper citation: RRID:Addgene_35287 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4850; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000086; Origin: SC101
Proper citation: RRID:Addgene_35320 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3703; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000052; Origin: colE1
Proper citation: RRID:Addgene_35286 Copy
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