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Species: Synthetic
Genetic Insert: Rep1 and Ref1
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35281741
Proper citation: RRID:Addgene_182054 Copy
Species: Synthetic
Genetic Insert: Rep6 and Ref6
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35281741
Proper citation: RRID:Addgene_182059 Copy
Species: Synthetic
Genetic Insert: attB-EGFP-EF1α-PuroR-T2A-TagBFP
Vector Backbone Description: Vector Backbone:pEF1α; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887556
Proper citation: RRID:Addgene_181923 Copy
Species: Synthetic
Genetic Insert: MiniE-DBCO
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32786411
Proper citation: RRID:Addgene_181985 Copy
Species: Synthetic
Genetic Insert: 7xFOP
Vector Backbone Description: Backbone Size:6957; Vector Backbone:pC0037; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31296651
Comments: From:
Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr Biol. 2003 Apr 15;13(8):680-5. doi: 10.1016/s0960-9822(03)00240-9. PMID: 12699626.
Proper citation: RRID:Addgene_181955 Copy
Species: Synthetic
Genetic Insert: attP-EGFP-EF1α-PuroR-T2A-TagBFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887556
Proper citation: RRID:Addgene_182139 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182282 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-M (cAMP sensor)
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182284 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L (cAMP sensor)
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182283 Copy
Species: Synthetic
Genetic Insert: Alb_A277c_attB pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887556
Comments: The parent vector used for cloning pegRNA constructs via Golden Gate has been deposited to Addgene previously. The name of the parent vector is pU6-pegRNA-GG-acceptor and the Addgene number is 132777.
Proper citation: RRID:Addgene_182145 Copy
Species: Synthetic
Genetic Insert: attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP
Vector Backbone Description: Vector Backbone:pEF1α; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887556
Proper citation: RRID:Addgene_182140 Copy
Species: Synthetic
Genetic Insert: IDS2-A4b-attP-fwd pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887556
Comments: The parent vector used for cloning pegRNA constructs via Golden Gate has been deposited to Addgene previously. The name of the parent vector is pU6-pegRNA-GG-acceptor and the Addgene number is 132777.
Proper citation: RRID:Addgene_182149 Copy
Species: Synthetic
Genetic Insert: CCR5-B584b-attB pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887556
Comments: The parent vector used for cloning pegRNA constructs via Golden Gate has been deposited to Addgene previously. The name of the parent vector is pU6-pegRNA-GG-acceptor and the Addgene number is 132777.
Proper citation: RRID:Addgene_182148 Copy
Species: Synthetic
Genetic Insert: IDS2-B7b-attP-fwd pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887556
Comments: The parent vector used for cloning pegRNA constructs via Golden Gate has been deposited to Addgene previously. The name of the parent vector is pU6-pegRNA-GG-acceptor and the Addgene number is 132777.
Proper citation: RRID:Addgene_182150 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L (cAMP sensor)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182279 Copy
Species: Synthetic
Genetic Insert: GFP 1-9 OPT
Vector Backbone Description: Backbone Marker:pEGFP (Clontech); Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24092409
Proper citation: RRID:Addgene_182244 Copy
Species: Synthetic
Genetic Insert: T2A-tdTomato-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182340 Copy
Species: Synthetic
Genetic Insert: T2A-TagRFP-T-linker-dre-PBD*-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182342 Copy
Species: Synthetic
Genetic Insert: mVenusNB-MrkHR113A
Vector Backbone Description: Backbone Marker:J. H. Davis, A. J. Rubin, R. T. Sauer, Design, construction and characterization of a set of insulated bacterial promoters.; Backbone Size:3200; Vector Backbone:pEB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35131853
Comments: We obtained the codon optimized MrkH sequence from Maria Schumacher, as described in her publication PMID: 27551088
Proper citation: RRID:Addgene_182292 Copy
Species: Synthetic
Genetic Insert: BSD
Vector Backbone Description: Backbone Marker:Alfred L. Fisher; Vector Backbone:pMOD4-galK-G; Vector Types:Bacterial Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: PCR amplify around the 5' T2A and 3' FRT site, making sure to add a PacI site at the 3' of the FRT site.
As suggested in the plasmid name, due to a PCR error, the rpsL open reading frame has a 1-bp deletion that results in a frame shift and as a consequence premature stop codons. Despite this, the downstream BSD open reading frame is translated from the bicistronic RNA in E. coli without problems. These mean that the mutated rpsL cannot be used for streptomycin counterselection, but the unmutated BSD can and was successfully used several times to confer blasticidin resistance for selection after recombineering.
Proper citation: RRID:Addgene_182338 Copy
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