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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pET22b-BirA-CPDSalI
 
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RRID:Addgene_38258 birA E. coli Ampicillin PMID:19956581 Encodes untagged biotin ligase gene (birA) from E. coli fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used to purify biotin ligase. Backbone Marker:Matthew Bogyo laboratory; Backbone Size:6126; Vector Backbone:Addgene Plasmid #38251 (pET22b-CPDSalI); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin cloned from pGEX4T1-BirA 2026-08-29 01:04:49 0
pInducer10-mir-RUP-PheS
 
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10+ mentions
RRID:Addgene_44011 PheS Gly294 E. coli Ampicillin PMID:21307310 Backbone Marker:Open Biosystems; Backbone Size:13167; Vector Backbone:pGIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:05:41 19
pSO221 Phis-72 BirA GFP
 
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RRID:Addgene_45100 BirA::GFP E. coli Ampicillin PMID:22219512 Backbone Size:2884; Vector Backbone:pWormgate2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:05:49 0
pBADmod1-linker-deGFP
 
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RRID:Addgene_45397 deGFP e. coli Ampicillin PMID:20576148 Backbone Marker:Invitrogen; Backbone Size:4102; Vector Backbone:pBAD/His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:05:51 0
pBEST-p15A-PLtetO1-deGFP-T500
 
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RRID:Addgene_45392 deGFP e. coli Ampicillin PMID:20576148 Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:05:57 0
pBEST-p15A-PLtetO1-tetR-T500
 
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RRID:Addgene_45391 tet repressor e. coli Ampicillin PMID:20576148 Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:05:51 0
pBEST-p15A-PLlacO1-deGFP-T500
 
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1+ mentions
RRID:Addgene_45394 deGFP e. coli Ampicillin PMID:20576148 Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:05:51 1
pSigma54-4
 
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RRID:Addgene_41748 rpoN E. coli Ampicillin PMID:22341441 Backbone Marker:novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin c198a c307g c346a 2026-08-29 01:05:19 0
pCMV-Rab-Rep
 
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RRID:Addgene_45967 beta-Galactosidase E. coli Ampicillin Backbone Marker:Clontech; Vector Backbone:pCMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin duplicated aa 1-135 2026-08-29 01:05:59 0
pCMV-TALEN-Rep
 
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1+ mentions
RRID:Addgene_45964 beta-Galactosidase E. coli Ampicillin Backbone Marker:Clontech; Vector Backbone:pCMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin duplicated aa 1-135 2026-08-29 01:05:59 3
pGR-ECK120033127
 
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RRID:Addgene_46011 ECK120033127 Terminator E. coli Ampicillin PMID:23727987 Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:05:56 0
pGR-ECK120010800
 
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RRID:Addgene_46010 ECK120010800 Terminator E. coli Ampicillin PMID:23727987 Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:05:56 0
pGR-BBa_B0010
 
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RRID:Addgene_46003 BBa_B0010 Terminator E. coli Ampicillin PMID:23727987 Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:05:59 0
pGR-ECK125108723
 
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RRID:Addgene_46008 ECK125108723 Terminator E. coli Ampicillin PMID:23727987 Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:05:56 0
pGR-ECK120010783
 
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RRID:Addgene_46009 ECK120010783 Terminator E. coli Ampicillin PMID:23727987 Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:05:59 0
pGR-ECK120015170
 
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RRID:Addgene_46006 ECK120015170 Terminator E. coli Ampicillin PMID:23727987 Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:05:59 0
pET21-Streptavidin-Dead Aspartate Loop
 
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RRID:Addgene_46368 Streptavidin-Dead Aspartate Loop E. coli Ampicillin PMID:24056174 Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:01 0
pAT222
 
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RRID:Addgene_40942 GFP-LacI** E. coli Ampicillin PMID:21724830 The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. Alternate plasmid name: pAT123LacI2G(4mutations) Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:5650; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin LacI**/LacI2G(4 mutations) (see comment below); lacI-I12 mutation (P3Y); C-terminal 11 amino acids deleted to prevent tetramerization 2026-08-29 01:05:10 0
R4-LexAp65-R3
 
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RRID:Addgene_41438 LexAp65 E. coli Kanamycin Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR P4r-P3r; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:05:16 0
pEQ111m
 
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1+ mentions
RRID:Addgene_40190 EcoRI endonuclease & EcoRI methylase E. coli Ampicillin PMID:2745418 pSCC3 plasmid derivation is described in King, K. et al. (1989) J.Biol.Chem. 264, 11807-11815. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pSCC3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changed Glutamic Acid 111 to Glutamine 2026-08-29 01:05:03 2

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