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Species: Synthetic
Genetic Insert: ABE7.10
Vector Backbone Description: Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29160308
Proper citation: RRID:Addgene_102919 Copy
Species: Synthetic
Genetic Insert: ABE6.3
Vector Backbone Description: Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29160308
Proper citation: RRID:Addgene_102916 Copy
Species: Synthetic
Genetic Insert: dSpCas9, pdDronpa1.2
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:pPB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28938067
Proper citation: RRID:Addgene_102855 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.B2 VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26829320
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_103003 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.B VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26829320
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_103002 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.eB VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_103005 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.B3 VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26829320
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_103004 Copy
Species: Synthetic
Genetic Insert: TIMER-bac
Vector Backbone Description: Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25126781
Comments: TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance.
Proper citation: RRID:Addgene_103057 Copy
Species: Synthetic
Genetic Insert: Fusicatenibacter saccharivorans RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116956 Copy
Species: Synthetic
Genetic Insert: Bacteroides fragilis strain S14 RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116957 Copy
Species: Synthetic
Genetic Insert: Bacteroides fragilis strain S14 RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116958 Copy
Species: Synthetic
Genetic Insert: Fusicatenibacter saccharivorans RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116952 Copy
Species: Synthetic
Genetic Insert: Fusicatenibacter saccharivorans RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116955 Copy
Species: Synthetic
Genetic Insert: C. elegans codon optimized GFPnovo2
Vector Backbone Description: Backbone Marker:Cori Bargmann's lab; Backbone Size:3600; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32550394
Comments: Please note that some discrepancies were found between Addgene's quality control and the depositor's full plasmid sequence. The depositor noted that the backbone was not sequenced, and these discrepancies do NOT affect plasmid function.
GFPnovo2 was originally generated by Arakawa et al., (2008)
Arakawa, H., Kudo, H., Batrak, V., Caldwell, R. B., Rieger, M. A., Ellwart, J. W., & Buerstedde, J.-M. (2008). Protein evolution by hypermutation and selection in the B cell line DT40. Nucleic Acids Research, 36(1), e1. http://doi.org/10.1093/nar/gkm616
Proper citation: RRID:Addgene_116943 Copy
Species: Synthetic
Genetic Insert: g3c
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30127387
Proper citation: RRID:Addgene_116893 Copy
Species: Synthetic
Genetic Insert: g3c
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30127387
Proper citation: RRID:Addgene_116891 Copy
Species: Synthetic
Genetic Insert: g3c
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30127387
Proper citation: RRID:Addgene_116892 Copy
Species: Synthetic
Genetic Insert: Azospirillum lipoferum 4B RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116981 Copy
Species: Synthetic
Genetic Insert: Woodsholea maritima RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116980 Copy
Species: Synthetic
Genetic Insert: Micromonospora rosaria RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116970 Copy
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