Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Synthetic
Genetic Insert: 3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-HF-BE3 from lacIq-Ptac promoter. Basal expression is sufficient for base editing in Coxiella burnetii. Note: During growth of E. coli harboring this plasmid, in some cells/colonies, the basal expression of 3xF-HF-BE3 can lead to spurious deamination of cytosines on the plasmid.
Unique XhoI, NotI and XbaI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid alias: pSAST356.
Proper citation: RRID:Addgene_221138 Copy
Species: Synthetic
Genetic Insert: 3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-BE4-PpAPOBEC1(H122A) from lacIq-Ptac promoter. IPTG induction is required for base editing in Coxiella burnetii. Unique XhoI, NotI and SapI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid encodes sacB, but sacB is not required for Coxiella burnetii base editing protocol. Plasmid alias: pSAST372.
Proper citation: RRID:Addgene_221139 Copy
Vector Backbone Description: Vector Backbone:pTcINDEX; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25424446
Comments: 5' Cloning Site: NruI (destroyed), 3' Cloning Site: NruI (destroyed).
Proper citation: RRID:Addgene_234713 Copy
Vector Backbone Description: Vector Backbone:pTcTurboID-GW; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: If you want to perform traditional cloning (instead of Gateway recombiantion), it can be achieved by replacing the GFP cassette from pTcTurboID-GW_GFP with the desired CDS using BamHI and EcoRV restriction enzymes. Also, any CDS can be cloned in frame to GFP using only EcoRV. 5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: ClaI (not destroyed).
Proper citation: RRID:Addgene_234714 Copy
Vector Backbone Description: Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40048245
Proper citation: RRID:Addgene_238493 Copy
Vector Backbone Description: Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40048245
Proper citation: RRID:Addgene_238494 Copy
Vector Backbone Description: Backbone Marker:https://www.addgene.org/73638/; Backbone Size:5643; Vector Backbone:pDEST-ORF-V2; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40419795
Proper citation: RRID:Addgene_217453 Copy
Vector Backbone Description: Backbone Marker:https://www.addgene.org/73636/; Backbone Size:7371; Vector Backbone:pDEST-V2-ORF; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40419795
Proper citation: RRID:Addgene_217454 Copy
Species: Danio rerio
Genetic Insert: 4xU6:sgRNA
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_227778 Copy
Species: Homo sapiens
Genetic Insert: SpCas9 codon optimized for human
Vector Backbone Description: Vector Backbone:pX330_42230; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39721357
Comments: Expression vector from Cong et al. 10.1126/science.1231143 that carries the chloramphenicol resistance protein ABO28845.1 and the negative selection marker ccdB ABO28846.1 for efficient cloning of sgRNA targeting sequences.
Proper citation: RRID:Addgene_223321 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222042 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222040 Copy
Vector Backbone Description: Vector Backbone:pGGD000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222067 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222066 Copy
Vector Backbone Description: Vector Backbone:pGGF000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222069 Copy
Vector Backbone Description: Backbone Marker:Alexander Stark (Addgene # 99292); Vector Backbone:hSTARR-seq_SCP1 vector; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39716078
Proper citation: RRID:Addgene_220289 Copy
Vector Backbone Description: Vector Backbone:pCAGEN_DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229834 Copy
Vector Backbone Description: Vector Backbone:pB-pRAM-Flex-mClover3-KrasG12D-WPRE-pA-EFS-TetR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229828 Copy
Genetic Insert: FRT10-STOP-FRT10
Vector Backbone Description: Vector Backbone:pBPGAL4DBD; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39677673
Comments: Please visit https://doi.org/10.1101/2024.12.02.626507 for bioRxiv preprint.
Proper citation: RRID:Addgene_232831 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229815 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
