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Vector Backbone Description: Vector Backbone:pTcTurboID-GW; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: If you want to perform traditional cloning (instead of Gateway recombiantion), it can be achieved by replacing the GFP cassette from pTcTurboID-GW_GFP with the desired CDS using BamHI and EcoRV restriction enzymes. Also, any CDS can be cloned in frame to GFP using only EcoRV. 5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: ClaI (not destroyed).
Proper citation: RRID:Addgene_234714 Copy
Vector Backbone Description: Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40048245
Proper citation: RRID:Addgene_238493 Copy
Vector Backbone Description: Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40048245
Proper citation: RRID:Addgene_238494 Copy
Vector Backbone Description: Backbone Size:10406; Vector Backbone:pDN0520; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210482 Copy
Vector Backbone Description: Backbone Size:10711; Vector Backbone:pKB11; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210487 Copy
Vector Backbone Description: Backbone Size:9355; Vector Backbone:pDN0520; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210483 Copy
Vector Backbone Description: Backbone Size:9604; Vector Backbone:pKB12; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210488 Copy
Vector Backbone Description: Backbone Size:10720; Vector Backbone:pDN0509; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210485 Copy
Vector Backbone Description: Backbone Marker:Dominic Esposito (Addgene plasmid # 11518); Backbone Size:8695; Vector Backbone:pDest-527; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39036966
Comments: Useful bacterial expression vector for purifying challenging proteins.
SuperHis (14xHis) ensures very high affinity to Ni-IMAC column.
mOCR tag is very negatively charged. This promotes proper folding, prevents aggregation and enables purification via ion-exchange.
Refer to DelProposto et al 2008 (DOI: 10.1016/j.pep.2008.08.011) for information on mOCR tag fusions.
Proper citation: RRID:Addgene_206874 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6636; Vector Backbone:pDESTattB; Vector Types:Zebrafish Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38838146
Comments: Please visit https://doi.org/10.1101/2023.12.08.570868 for bioRxiv preprint.
Proper citation: RRID:Addgene_213509 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Swapped tags from pcDNA3.1-ccdB-3xFLAG-V5 (addgene #87063)
Proper citation: RRID:Addgene_214985 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Swapped tags from pcDNA3.1-ccdB-3xFLAG-V5 (addgene #87063)
Proper citation: RRID:Addgene_214988 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7061; Vector Backbone:pDESTattB; Vector Types:Zebrafish Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38838146
Comments: Please visit https://doi.org/10.1101/2023.12.08.570868 for bioRxiv preprint.
Proper citation: RRID:Addgene_213511 Copy
Species: Danio rerio
Genetic Insert: 4xU6:sgRNA
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_227778 Copy
Species: Homo sapiens
Genetic Insert: SpCas9 codon optimized for human
Vector Backbone Description: Vector Backbone:pX330_42230; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39721357
Comments: Expression vector from Cong et al. 10.1126/science.1231143 that carries the chloramphenicol resistance protein ABO28845.1 and the negative selection marker ccdB ABO28846.1 for efficient cloning of sgRNA targeting sequences.
Proper citation: RRID:Addgene_223321 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222042 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222040 Copy
Vector Backbone Description: Vector Backbone:pGGD000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222067 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222066 Copy
Vector Backbone Description: Vector Backbone:pGGF000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222069 Copy
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