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Species: E. coli
Genetic Insert: plsB, plsC, cdsA, pssA
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC57-oriL191-oriR194; Vector Types:Cell-free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40471122
Comments: Please visit https://doi.org/10.1101/2024.03.22.586135 for bioRxiv preprint.
Proper citation: RRID:Addgene_216483 Copy
Species: E. coli
Genetic Insert: mitoEcSTH
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9227; Vector Backbone:pLVX-TetOne-Puro; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37884806
Proper citation: RRID:Addgene_218629 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS dtyrTV::tolC tyrU::gentR lambda::ZeoR
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218770 Copy
Species: E. coli
Genetic Insert: EcTyrRS-VSMA*
Vector Backbone Description: Backbone Size:4091; Vector Backbone:pEVOL-tac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38913984
Comments: EcY-tRNA-TAG
After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.
Proper citation: RRID:Addgene_218766 Copy
Species: E. coli
Genetic Insert: EcWRS-h9
Vector Backbone Description: Backbone Size:4166; Vector Backbone:pEvoltac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28192410
Comments: EcW-tRNA-TGA
After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.
Proper citation: RRID:Addgene_218765 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS tyrTV::inact tyrU::inact lambda::tolC
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218773 Copy
Species: E. coli
Genetic Insert: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)
Defining Citation: PMID:38913984
Proper citation: RRID:Addgene_218774 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::inact lambda::tolC
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218771 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::CUA lambda::tolC
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218772 Copy
Species: E. Coli
Genetic Insert: Paph(3')-I_[abr]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_225770 Copy
Species: E. coli
Genetic Insert: PmarC9_[Himar]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_225800 Copy
Species: E. coli
Genetic Insert: speG
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38885650
Proper citation: RRID:Addgene_220483 Copy
Species: E. coli
Genetic Insert: GST
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19797087
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146223 Copy
Species: E. coli
Genetic Insert: MBP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19797087
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146486 Copy
Species: E. coli
Genetic Insert: human RNaseH1 wild type
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36544021
Proper citation: RRID:Addgene_196702 Copy
Species: E. coli
Genetic Insert: wt E. coli SSB
Vector Backbone Description: Backbone Marker:EMB/Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24021816
Proper citation: RRID:Addgene_197260 Copy
Species: E. coli
Genetic Insert: bacterial RNaseHI
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36544021
Proper citation: RRID:Addgene_196701 Copy
Species: E. coli
Genetic Insert: MscL(G22S)-c-term-cpGFP-ERexp
Vector Backbone Description: Backbone Size:6100; Vector Backbone:Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608338
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.04.510821v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_195346 Copy
Species: E. coli
Genetic Insert: tRNAfMet-A37U expression cassette
Vector Backbone Description: Vector Backbone:pSEVA361; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36727459
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.05.02.490318v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_196659 Copy
Species: E. coli
Genetic Insert: MSP1E3D1_mBlueberry2
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248930 Copy
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