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Species: Synthetic
Genetic Insert: sZF6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43837 Copy
Species: Synthetic
Genetic Insert: sZF7
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43838 Copy
Species: Synthetic
Genetic Insert: GCaMP3
Vector Backbone Description: Backbone Marker:Addgene plasmid 43915; Backbone Size:9600; Vector Backbone:FU-MAP2-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22174877
Comments: The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway (Addgene plasmid #43915).
This destination vector was subsequently used to generate MAP2-GCaMP3 (GCaMP3 cloned from G-CaMP3, Addgene plasmid 22692).
The V5 and His tags present in the vector backbone are not expressed with the GCaMP3 insert.
Proper citation: RRID:Addgene_43917 Copy
Species: Synthetic
Genetic Insert: HRP
Vector Backbone Description: Backbone Size:6142; Vector Backbone:pCTcon2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17961829
Proper citation: RRID:Addgene_44021 Copy
Species: Synthetic
Genetic Insert: Laconic (LACtate Optical Nano Indicator from CECs)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5418; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression, lactate biosensor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23469056
Comments: Laconic is a genetically-encoded biosensor that detects lactate in the physiological range. The sensor was based on LldR, a bacterial transcription regulator that consists of a lactate-binding/regulatory domain and a DNA-binding domain. Laconic consists of a N-terminal mTFP, followed by the LldR flanked by linkers, and finally a C-terminal Venus.
See Addgene plasmid# 46307: NLS-Laconic/pCMV-myc-nuc for a nuclear-targeted version of Laconic: http://www.addgene.org/46307/ .
Proper citation: RRID:Addgene_44238 Copy
Species: Synthetic
Genetic Insert: CBF1-Responsive-Element
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4540; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23617465
Proper citation: RRID:Addgene_44211 Copy
Species: Synthetic
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3998; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44275 Copy
Species: Synthetic
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6640; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44278 Copy
Species: Synthetic
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5072; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44277 Copy
Species: Synthetic
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44279 Copy
Species: Synthetic
Genetic Insert: PAiRFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4064; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23842578
Proper citation: RRID:Addgene_44271 Copy
Species: Synthetic
Genetic Insert: jGCaMP7b-XC
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36196992
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.09.475579v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178361 Copy
Species: Synthetic
Genetic Insert: Lactate 2-monooxygenase
Vector Backbone Description: Backbone Size:11848; Vector Backbone:pXCX; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34439675
Proper citation: RRID:Addgene_178312 Copy
Species: Synthetic
Genetic Insert: D-lactate dehydrogenase
Vector Backbone Description: Backbone Size:11870; Vector Backbone:pXCX; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34439675
Proper citation: RRID:Addgene_178311 Copy
Species: Synthetic
Genetic Insert: td-sfGFP
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, FLP/FRT, piggyBac; Bacterial Resistance:Ampicillin
Comments: Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
Proper citation: RRID:Addgene_178433 Copy
Species: Synthetic
Genetic Insert: Stargazin-mEGFP-iLID
Vector Backbone Description: Backbone Size:3294; Vector Backbone:pTol2 (tol2 transposase donor vector); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34880255
Comments: The sequence and plasmid map is available in the following benchling link (https://benchling.com/s/seq-rBh93iqQQT5hBXFNIIVG).
Proper citation: RRID:Addgene_178529 Copy
Species: Synthetic
Genetic Insert: EF1a-NlaCas7c-bGH PolyA
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1993; Vector Backbone:pSmart HC Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_178879 Copy
Species: Synthetic
Genetic Insert: PB1-MontiRed-LOV2dark
Vector Backbone Description: Backbone Marker:Dr. Jun-ichi Miyazaki (Osaka University, Japan); Backbone Size:4091; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33890764
Proper citation: RRID:Addgene_178874 Copy
Species: Synthetic
Genetic Insert: EF1a-NlaCas5c-bGH polyA
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1984; Vector Backbone:pSmart HC Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_178878 Copy
Species: Synthetic
Genetic Insert: PB1-AzamiGreen(Y63L)-LOV2
Vector Backbone Description: Backbone Marker:Dr. Jun-ichi Miyazaki (Osaka University, Japan); Backbone Size:4091; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33890764
Proper citation: RRID:Addgene_178876 Copy
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