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Vector Backbone Description: Vector Backbone:pDestTol2pA2; Vector Types:Other, Other; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36975217
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint.
Proper citation: RRID:Addgene_195984 Copy
Species: Homo sapiens
Genetic Insert: Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A)
Vector Backbone Description: Backbone Marker:None; Backbone Size:11000; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33547076
Proper citation: RRID:Addgene_176291 Copy
Vector Backbone Description: Backbone Marker:None; Backbone Size:10000; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33547076
Proper citation: RRID:Addgene_176296 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229815 Copy
Vector Backbone Description: Vector Backbone:pTol2-Hsp70-zCreI-mTagBFP2d-2x-ins; Vector Types:Tol2 destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229817 Copy
Vector Backbone Description: Vector Backbone:GLAST-pBase; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229822 Copy
Vector Backbone Description: Vector Backbone:pB_Tet-Off_Flex_DEST_EFS_mODC_tTA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229821 Copy
Genetic Insert: epegRNA
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40555761
Comments: Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint.
Proper citation: RRID:Addgene_240539 Copy
Species: Synthetic
Genetic Insert: attR1-CmR-ccdB-attR2
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242575 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242573 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242577 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11967; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF2 transcription factor followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173728 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11484; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 20 copies of the UAS binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173723 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11397; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 13 copies of the LexA binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173724 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11189; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 10 copies of the UAS binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173725 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:12063; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a LexA:QF hybrid transcription factor (LexA DNA binding domain, QF activation domain) followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173727 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:13668; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF:GAL4 hybrid transcription factor (QF DNA binding domain, Gal4 activation domain) followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173729 Copy
Vector Backbone Description: Backbone Marker:https://www.addgene.org/73638/; Backbone Size:5643; Vector Backbone:pDEST-ORF-V2; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40419795
Proper citation: RRID:Addgene_217453 Copy
Vector Backbone Description: Backbone Marker:https://www.addgene.org/73636/; Backbone Size:7371; Vector Backbone:pDEST-V2-ORF; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40419795
Proper citation: RRID:Addgene_217454 Copy
Vector Backbone Description: Vector Backbone:pTcINDEX; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25424446
Comments: 5' Cloning Site: NruI (destroyed), 3' Cloning Site: NruI (destroyed).
Proper citation: RRID:Addgene_234713 Copy
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