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Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197800 Copy
Vector Backbone Description: Vector Backbone:Yep351; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25066056
Proper citation: RRID:Addgene_203164 Copy
Vector Backbone Description: Vector Backbone:pVV208; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34382076
Proper citation: RRID:Addgene_203176 Copy
Vector Backbone Description: Backbone Size:9748; Vector Backbone:plex_306; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201135 Copy
Vector Backbone Description: Backbone Size:10986; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201125 Copy
Vector Backbone Description: Backbone Size:10580; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201126 Copy
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_198637 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.
Proper citation: RRID:Addgene_199082 Copy
Vector Backbone Description: Vector Backbone:pAG416GALL-ccdB-6stop; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39762652
Proper citation: RRID:Addgene_203511 Copy
Vector Backbone Description: Vector Backbone:pAG416GAL-2x-uA-ccdB-6stop; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39762652
Proper citation: RRID:Addgene_203517 Copy
Vector Backbone Description: Backbone Size:10995; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: K207A mutation in EYFP will not affect plasmid function.
Proper citation: RRID:Addgene_201128 Copy
Vector Backbone Description: Backbone Size:10602; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: K207A mutation in EYFP will not affect plasmid function.
Proper citation: RRID:Addgene_201131 Copy
Vector Backbone Description: Backbone Size:9355; Vector Backbone:pDN0520; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210483 Copy
Vector Backbone Description: Backbone Size:9604; Vector Backbone:pKB12; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210488 Copy
Vector Backbone Description: Backbone Size:10720; Vector Backbone:pDN0509; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210485 Copy
Vector Backbone Description: Backbone Marker:Dominic Esposito (Addgene plasmid # 11518); Backbone Size:8695; Vector Backbone:pDest-527; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39036966
Comments: Useful bacterial expression vector for purifying challenging proteins.
SuperHis (14xHis) ensures very high affinity to Ni-IMAC column.
mOCR tag is very negatively charged. This promotes proper folding, prevents aggregation and enables purification via ion-exchange.
Refer to DelProposto et al 2008 (DOI: 10.1016/j.pep.2008.08.011) for information on mOCR tag fusions.
Proper citation: RRID:Addgene_206874 Copy
Species: Synthetic
Genetic Insert: mCherry-FLAG-cat
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Proper citation: RRID:Addgene_211831 Copy
Species: Danio rerio
Genetic Insert: nrg1 type I sgRNA
Vector Backbone Description: Vector Backbone:pDestTol2CG2-U6:gRNA; Vector Types:Tol2 destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36384112
Proper citation: RRID:Addgene_199387 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197841 Copy
Vector Backbone Description: Backbone Size:10157; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201122 Copy
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