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Species: Caenorhabditis elegans
Genetic Insert: beta-tubulin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Comments: 1st generation pie-1 vector
Proper citation: RRID:Addgene_26951 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRA-2 GFP
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pPD95.79; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20921392
Proper citation: RRID:Addgene_27128 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRA-2 FLAG
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pPD95.79; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20921392
Comments: Cloning of this plasmid was achieved in the following steps:
(1)the sequence 3' (3'UTR) of the tags was added by (EcoRI/SpeI)
(2) 1.2 kb XhoI/KpnI tra-2 pcr fragment into SalI/KpnI cut vector (destroying one SalI/XhoI site but adding another in the insert) immediately 5' of the tag
(3) 7 kb HindIII/SalI fragment into same that partially replaced fragment added in step 2 containing most of the TRA-2 coding sequence
(4) 5 kb Xho/AgeI that added the promoter and upstream gene in the operon as well as some Tra-2 coding sequence
Proper citation: RRID:Addgene_27129 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmyo-3::cfp::lacZ::unc-54_3'UTR
Vector Backbone Description: Backbone Size:2000; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Comments: Please note that there are some slight discrepancies between Addgene's quality control sequence and the theoretical sequence from the Fire lab. These differences should not affect function.
Proper citation: RRID:Addgene_27510 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:TOPO; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Proper citation: RRID:Addgene_27504 Copy
Species: Caenorhabditis elegans
Genetic Insert: grh-1
Vector Backbone Description: Vector Backbone:pdestMB14 (Addgene #26415); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35013237
Proper citation: RRID:Addgene_177761 Copy
Species: Caenorhabditis elegans
Genetic Insert: K+-channel KCNQ
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pBScMXT; Vector Types:Xenopus Oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10559392
Proper citation: RRID:Addgene_16209 Copy
Species: Caenorhabditis elegans
Genetic Insert: eag-like K+ channels
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pOX; Vector Types:Xenopus Oocyte expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_16213 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc4-rtTA(Q)
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164891 Copy
Species: Caenorhabditis elegans
Genetic Insert: CFP
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164894 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164885 Copy
Species: Caenorhabditis elegans
Genetic Insert: zip-QFAD
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164888 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prig-3, tagBFP
Vector Backbone Description: Backbone Marker:Anuj Sharma; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34323218
Comments: Please visit https://www.biorxiv.org/content/10.1101/445643v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_165598 Copy
Species: Caenorhabditis elegans
Genetic Insert: PCYC1_SEC61_mCherry_LEU2_TCYC1
Vector Backbone Description: Backbone Size:4872; Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32907940
Proper citation: RRID:Addgene_170359 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171206 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171202 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-3
Vector Backbone Description: Vector Backbone:pMAL-c2X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171209 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166972 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166975 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166974 Copy
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