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Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5519; Vector Backbone:pRExLIC; Vector Types:Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47078 Copy
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:6247; Vector Backbone:pRExLIC; Vector Types:Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47077 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5824; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47069 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47067 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pMT/BiP/V5/His A; Vector Types:Insect Expression, Secreted expression in Drosophila Culture; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23827685
Comments: Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006
After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination.
Proper citation: RRID:Addgene_47051 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4349; Vector Backbone:pMT/BiP/V5/His A; Vector Types:Insect Expression, Secreted expression in Drosophila Culture; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23827685
Comments: Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006
After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination.
Proper citation: RRID:Addgene_47032 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR4; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23979020
Comments: For more information including protocols and
updates, please go to http://www.crispr-on.org
Proper citation: RRID:Addgene_48234 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36433 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36434 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36435 Copy
Genetic Insert: Gateway Cassette A R2-R1
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36428 Copy
Vector Backbone Description: Backbone Marker:Voytas lab (Addgene Plasmid #35397); Backbone Size:13853; Vector Backbone:pCP5; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21493687
Comments: pCP5b is a high copy derivative of pCP5 for higher DNA yield from bacteria.
Plasmid used to assay TALEN function in yeast. Clone target sequences into BglII and SpeI sites as described on article.
Grow yeast transformants in SD-Ura-Trp prior to assay to prevent spontaneous recombination between the lacZ fragments
Proper citation: RRID:Addgene_37185 Copy
Vector Backbone Description: Backbone Size:10184; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23193063
Comments: pCEP4 is from Invitrogen. CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. Gateway cassette is from Invitrogen.
Proper citation: RRID:Addgene_37626 Copy
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18988847
Proper citation: RRID:Addgene_41941 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1::[(G4S)3]::C-TAP Tag
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42166 Copy
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42153 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42154 Copy
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42155 Copy
Genetic Insert: CFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42151 Copy
Genetic Insert: TALE-TF(VP64)-BB
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Adeno-associated Virus (); Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22828628
Comments: This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.
Proper citation: RRID:Addgene_42581 Copy
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