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Species: Mus musculus
Genetic Insert: Sprouty-2 shRNA
Vector Backbone Description: Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17369402
Comments: The sequence of the Spry-2 shRNA is 5'-GCCGG GTTGT CGTTG TAAA-3' and corresponds to nucleotides 1150–1168 of mSpry-2.
Proper citation: RRID:Addgene_14809 Copy
Species: Homo sapiens
Genetic Insert: Retinoblastoma Binding Protein 2
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17320163
Comments: The plasmid encodes isoform 2.
Proper citation: RRID:Addgene_14803 Copy
Species: Mus musculus
Genetic Insert: K-ras 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Proper citation: RRID:Addgene_14804 Copy
Species: Mus musculus
Genetic Insert: Dicer shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: GATCCCCCTG CCTCACTTGA CCTGAATTCA AGAGATTCAG GTCAAGTGAG GCAGTTTTTG GAAC
Proper citation: RRID:Addgene_14779 Copy
Species: Drosophila melanogaster
Genetic Insert: DmNot1_812-1945-dsRNAres
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24768540
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147845 Copy
Species: Mus musculus
Genetic Insert: Dicer shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: GATCCCCGCA TGGTGGTGTC GATATTTTCA AGAGAAATAT CGACACCACC ATGCTTTTTG GAAC
Proper citation: RRID:Addgene_14778 Copy
Species: Mus musculus
Genetic Insert: PERP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14707288
Comments: The pPERPlucFL vector was constructed by insertion of a splice acceptor upstream of the luciferase cassette in pGL3Basic and insertion of a BamHI Perp genomic fragment (from -3850 to +4150) into the polylinker region.
Proper citation: RRID:Addgene_14811 Copy
Species: Drosophila melanogaster
Genetic Insert: DmThor_50-83-V75DL79D
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25702871
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148379 Copy
Species: Homo sapiens
Genetic Insert: HsNot4
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30692204
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148255 Copy
Species: Rattus norvegicus
Genetic Insert: Syntaxin2D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:PCDNA4/TO/myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12737809
Proper citation: RRID:Addgene_14818 Copy
Species: Rattus norvegicus
Genetic Insert: Syntaxin2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:PCDNA4/TO/myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12737809
Proper citation: RRID:Addgene_14817 Copy
Species: Homo sapiens
Genetic Insert: MKK3
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4952; Vector Backbone:pGex 3x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7839144
Proper citation: RRID:Addgene_14814 Copy
Species: Rattus norvegicus
Genetic Insert: DAG reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: CFP and YFP fused to the C1A and C1B domains of PKCbetaII. DAGR was created by PCR of the C1A and C1B domains of rat PKCßII (aa 37–152) from KpnI to XhoI and substitution for the PH domain in CYPHR.
Proper citation: RRID:Addgene_14865 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: Phosphoacceptor threonine in the PKC substrate sequence changed to alanine. PKC substrate sequence flanked by flexible linker sequence: GGSGG RFRRFQALKIKAKA GGSGG. See CKAR for additional information.
Proper citation: RRID:Addgene_14861 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: The MyrPalm tag in this plasmid uses the Lyn kinase amino terminal sequence of MGCIKSK from UniProtKB reference sequence P07948.3. This sequence encodes for myristoylation and palmitoylation, shown previously to be sufficient to target a protein to the plasma membrane.
Proper citation: RRID:Addgene_14862 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: PKC substrate sequence flanked by flexible linker sequence: GGSGG RFRRFQTLKIKAKA GGSGG. A206K mutation present in both CFP (mCFP) and citrine (mYFP) to reduce the intrinsic homoaffinity of all GFPs and preclude intermolecular FRET by CFP-YFP dimerization. CFP was amplified by PCR from a plasmid template to encode a HindIII restriction site followed by a consensus initiation site for translation (CGCCACC) before the initiating ATG of CFP, and a KpnI restriction site at the 3' end instead of a terminating codon. FHA2, a gift from Michael Yaffe (MIT, Cambridge, MA) was amplified by PCR to include KpnI and BamHI restriction sites at the 5' and 3' ends, respectively. Citrine was amplified by PCR to include a 5' BamHI followed by the PKC substrate sequence and a 3' XbaI following the terminating codon.
Proper citation: RRID:Addgene_14860 Copy
Species: Homo sapiens
Genetic Insert: HsRck_296-472-10xmut
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27009120
Comments: ORF extracted from NCBI: NM_004397.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148420 Copy
Species: Homo sapiens
Genetic Insert: HseIF4E
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27773676
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148669 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: NucCKAR was generated by fusing sequences encoding a basic nuclear localization signal, PKKRKVEDA, to the 3'-end of CKAR .
Proper citation: RRID:Addgene_14869 Copy
Genetic Insert: B kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15583002
Comments: PKB substrate sequence: RKRDRLGTLGI.
Proper citation: RRID:Addgene_14877 Copy
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