Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Saccharomyces cerevisiae
Genetic Insert: TIR1
Vector Backbone Description: Vector Backbone:ClhN-MET15; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088313
Proper citation: RRID:Addgene_207016 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HFL1
Vector Backbone Description: Vector Backbone:BS-ZJLEU; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088313
Proper citation: RRID:Addgene_207024 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pho8
Vector Backbone Description: Vector Backbone:BS-TRP1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088313
Proper citation: RRID:Addgene_207031 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cps1
Vector Backbone Description: Vector Backbone:BS-TRP1- PGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088313
Proper citation: RRID:Addgene_207030 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ypt1
Vector Backbone Description: Vector Backbone:pProEx; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39255801
Proper citation: RRID:Addgene_224265 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7-mGold
Vector Backbone Description: Vector Backbone:pUSE-URA3; Vector Types:Gene replacement vector for yeast; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218967 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Npl4 D460K/Y461A
Vector Backbone Description: Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36736315
Proper citation: RRID:Addgene_226126 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Npl4 W252A/R253E
Vector Backbone Description: Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36736315
Proper citation: RRID:Addgene_226125 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: His6-3C-Cdc48
Vector Backbone Description: Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36736315
Proper citation: RRID:Addgene_226121 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: His6-GST-3C-Npl4
Vector Backbone Description: Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36736315
Proper citation: RRID:Addgene_226127 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ufd1R64C-3C-His6
Vector Backbone Description: Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36736315
Proper citation: RRID:Addgene_226131 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GES
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Also expresses Idi1 and a mutant Erg20 (F96W, N127W) from Saccharomyces cerevisiae. Idi1, Erg20(F96W, N127W), and GES are targeted to the peroxisome with ePTS1. This plasmid also expresses a cytosolic, WT Erg20 synthetic homodimer in which two WT versions of the Erg20 gene are combined onto one transcript with a 6x serine-glycine linker. Integrated into the URA3 locus.
Proper citation: RRID:Addgene_218590 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Erg12
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:PSG749; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Also expresses Erg8p and Mvd1p from Saccharomyces cerevisiae. Integrated into the YDR514C locus
Proper citation: RRID:Addgene_218587 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GES
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Also expresses Idi1 and a mutant Erg20 (F96W, N127W) from Saccharomyces cerevisiae. Integrated into the URA3 locus.
Proper citation: RRID:Addgene_218589 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pex15
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Express Pex15, Pex22, Pex4, Pex1, and Pex6 into the URA3 locus
Proper citation: RRID:Addgene_218616 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pex13
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP137; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Express Pex13, Pex14, Pex17, Pex2, Pex10, and Pex12 into the LEU2 locus
Proper citation: RRID:Addgene_218615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pex5
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:PSG747; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Express Pex5, Pex3, Pex19, and Pex8 into the YBR197C locus
Proper citation: RRID:Addgene_218618 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pex30
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP137; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Integrated into LEU2 locus
Proper citation: RRID:Addgene_218610 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pex22
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP137; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Expresses Pex22(1-36)-mRuby2 which directs mRuby2 to the peroxisome membrane. Also overexpresses Pex11p. Integrated into the LEU2 locus
Proper citation: RRID:Addgene_218593 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:PSG746; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39402374
Comments: Expresses the components for the degron assay: UbiY(N-terminal degradation tag)-YFP-ePTS1, directing the YFP to the peroxisome. Integrated into the IAI11 locus.
Proper citation: RRID:Addgene_218594 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.