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Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26850639
Proper citation: RRID:Addgene_99911 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26850639
Proper citation: RRID:Addgene_99912 Copy
Vector Backbone Description: Vector Backbone:pCAGEN_DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229834 Copy
Vector Backbone Description: Vector Backbone:pB-pRAM-Flex-mClover3-KrasG12D-WPRE-pA-EFS-TetR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229828 Copy
Genetic Insert: FRT10-STOP-FRT10
Vector Backbone Description: Vector Backbone:pBPGAL4DBD; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39677673
Comments: Please visit https://doi.org/10.1101/2024.12.02.626507 for bioRxiv preprint.
Proper citation: RRID:Addgene_232831 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229815 Copy
Vector Backbone Description: Vector Backbone:pTol2-Hsp70-zCreI-mTagBFP2d-2x-ins; Vector Types:Tol2 destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229817 Copy
Vector Backbone Description: Vector Backbone:GLAST-pBase; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229822 Copy
Vector Backbone Description: Vector Backbone:pB_Tet-Off_Flex_DEST_EFS_mODC_tTA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229821 Copy
Vector Backbone Description: Vector Backbone:plix403; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223955 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223953 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223951 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223952 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223950 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223948 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223949 Copy
Species: Synthetic
Genetic Insert: 3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-HF-BE3 from lacIq-Ptac promoter. Basal expression is sufficient for base editing in Coxiella burnetii. Note: During growth of E. coli harboring this plasmid, in some cells/colonies, the basal expression of 3xF-HF-BE3 can lead to spurious deamination of cytosines on the plasmid.
Unique XhoI, NotI and XbaI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid alias: pSAST356.
Proper citation: RRID:Addgene_221138 Copy
Species: Synthetic
Genetic Insert: 3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-BE4-PpAPOBEC1(H122A) from lacIq-Ptac promoter. IPTG induction is required for base editing in Coxiella burnetii. Unique XhoI, NotI and SapI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid encodes sacB, but sacB is not required for Coxiella burnetii base editing protocol. Plasmid alias: pSAST372.
Proper citation: RRID:Addgene_221139 Copy
Species: Danio rerio
Genetic Insert: 4xU6:sgRNA
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_227778 Copy
Species: Homo sapiens
Genetic Insert: SpCas9 codon optimized for human
Vector Backbone Description: Vector Backbone:pX330_42230; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39721357
Comments: Expression vector from Cong et al. 10.1126/science.1231143 that carries the chloramphenicol resistance protein ABO28845.1 and the negative selection marker ccdB ABO28846.1 for efficient cloning of sgRNA targeting sequences.
Proper citation: RRID:Addgene_223321 Copy
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