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Vector Backbone Description: Backbone Marker:Addgene #91798; Backbone Size:9621; Vector Backbone:pLEX_305-C-dTAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37994369
Proper citation: RRID:Addgene_221465 Copy
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction.
Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)
Proper citation: RRID:Addgene_166139 Copy
Species: N. furzeri
Genetic Insert: DDX5ΔPrD(1-535)
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166150 Copy
Species: N. furzeri
Genetic Insert: DDX5
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166152 Copy
Species: N. furzeri
Genetic Insert: DDX5ΔIDR(1-483)
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166151 Copy
Species: N. furzeri
Genetic Insert: DDX5
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166149 Copy
Vector Backbone Description: Vector Backbone:plix403; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223955 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223953 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223951 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223952 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223950 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223948 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223949 Copy
Species: Synthetic
Genetic Insert: 3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-HF-BE3 from lacIq-Ptac promoter. Basal expression is sufficient for base editing in Coxiella burnetii. Note: During growth of E. coli harboring this plasmid, in some cells/colonies, the basal expression of 3xF-HF-BE3 can lead to spurious deamination of cytosines on the plasmid.
Unique XhoI, NotI and XbaI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid alias: pSAST356.
Proper citation: RRID:Addgene_221138 Copy
Species: Synthetic
Genetic Insert: 3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-BE4-PpAPOBEC1(H122A) from lacIq-Ptac promoter. IPTG induction is required for base editing in Coxiella burnetii. Unique XhoI, NotI and SapI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid encodes sacB, but sacB is not required for Coxiella burnetii base editing protocol. Plasmid alias: pSAST372.
Proper citation: RRID:Addgene_221139 Copy
Vector Backbone Description: Backbone Size:10359; Vector Backbone:pMuLE Lenti Dest Neo; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30061623
Proper citation: RRID:Addgene_84575 Copy
Vector Backbone Description: Backbone Size:15069; Vector Backbone:Lenti-CRISPR; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27458201
Proper citation: RRID:Addgene_85402 Copy
Genetic Insert: CreER
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pT3; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28009940
Proper citation: RRID:Addgene_85577 Copy
Genetic Insert: CreER
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pT3; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28009940
Proper citation: RRID:Addgene_85578 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85723 Copy
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