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Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The original human STARR-seq plasmid has been used in the Arnold et al. 2013 publication. It should be replaced for all screens in mammalian cells with the updated version reported by Muerdter et al. 2017 for much improved signal-to-noise, especially for screens with highly complex libraries (e.g. those covering an entire mammalian genome). The improved version is Addgene plasmid # 99296. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).
The sequence between SacI and AfeI on pGL4 was replaced by the fragment containing the Super Core Promoter 1 (SCP1), a synthetic intron (pIRESpuro3, Clontech; cat. no. 631619) an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms, and the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71509 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62176 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62177 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62178 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62175 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4884; Vector Backbone:pBluescript; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62296 Copy
Vector Backbone Description: Backbone Size:6354; Vector Backbone:pDestTol2pA2 (Tol2Kit#394); Vector Types:CRISPR, Tol2 destination vector for Gateway; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25752963
Comments: Reference for the Tol2Kit:
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007). The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236, 3088-3099.
Proper citation: RRID:Addgene_63157 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 region
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Unpublished MosSCI reagents from the Jorgensen lab.
This vector targets the site ttTi4348 on Chr. I.
Use with strain EG6701 at the CGC.
Note - plasmid contains TWO M13F primer sites.
Proper citation: RRID:Addgene_30538 Copy
Species: Homo sapiens
Genetic Insert: CD4-tdGFP
Vector Backbone Description: Backbone Size:10193; Vector Backbone:pAPIC-HIH; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21606367
Comments: Gateway destination vector for cloning any enhancer to drive expression of membrane marker CD4-tdGFP
The depositing laboratory recommends growing bacteria either on LB plates with 80 ug/ml Carbenicillin or in LB liquid media with 60 ug/ml Carbenicillin (a semi-synthetic ampicillin analog)
Proper citation: RRID:Addgene_31221 Copy
Genetic Insert: TALE Nuclease, NG for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32190 Copy
Genetic Insert: TALE Transcriptional Activator Backbone, NI for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32187 Copy
Genetic Insert: TALE Transcriptional Activator Backbone, NN for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32185 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.
Proper citation: RRID:Addgene_32318 Copy
Vector Backbone Description: Backbone Marker:Mo Bi Tec; Vector Backbone:pET01; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18930792
Proper citation: RRID:Addgene_32484 Copy
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was assembled and may contain minor discrepancies, which should not affect the function of the plasmid.
Proper citation: RRID:Addgene_32684 Copy
Vector Backbone Description: Backbone Size:8453; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Proper citation: RRID:Addgene_32682 Copy
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was digitally assembled and may contain minor discrepancies, which should not effect the function of the plasmid.
Proper citation: RRID:Addgene_32685 Copy
Vector Backbone Description: Backbone Size:4427; Vector Backbone:pACAGW-ChR2-Venus-AAV; Vector Types:AAV, aav; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32671 Copy
Vector Backbone Description: Vector Backbone:pcg2.0; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31308540
Proper citation: RRID:Addgene_180221 Copy
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:7603; Vector Backbone:pCDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35182477
Proper citation: RRID:Addgene_182926 Copy
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