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http://www.addgene.org/34916

Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms. This vector contains a Pmyo-2::EGFP::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN40-R4R3 which has a red fluorescent pharyngeal marker. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34916 Copy   

•   Source: Addgene

http://www.addgene.org/35205

Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:12020; Vector Backbone:pBID-UASC-MRG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Nterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.

Proper citation: RRID:Addgene_35205 Copy   

•   Source: Addgene

http://www.addgene.org/40239

Species: Synechococcus elongatus PCC 7942
Genetic Insert: Cm Resistance, neutral site II integration platform for Synechococcus
Vector Backbone Description: Vector Backbone:1572; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10812624
Comments: NSII vector cloning sites: NheI, XhoI, SmaI, XbaI, StuI, SalI, and EcoRV. Chloramphenicol cassette from pUC1819 (Cm resistant) as a BamHI fragment into BglII site of pAM1572 such that it reads away from the MCS and a ScaI digest gives a 3.9kb band. NSII vector; unique cloning sites NheI, XhoI, SmaI, XbaI, StuI, SalI, EcoRV.

Proper citation: RRID:Addgene_40239 Copy   

•   Source: Addgene

http://www.addgene.org/40241

Species: Synechococcus elongatus PCC 7942
Genetic Insert: promoterless luxAB reporter vector
Vector Backbone Description: Vector Backbone:1573; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10812624
Comments: NSII vector Lux Genes: luxAB cloning sites: NheI, XhoI, SmaI, XbaI, StuI, and SalI. luxAB as SalI-PvuII from pLAVI (pAM884) into EcoRV-SalI cut pAM1573. SalI, StuI, XbaI, SmaI, XhoI, NheI still unique. Glows in E. coli.

Proper citation: RRID:Addgene_40241 Copy   

•   Source: Addgene

http://www.addgene.org/40728

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5488; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Please note that the hygromycin cassette in this plasmid is non-functional. See provided protocol for high-throughput, ligation-independent cloning using this plasmid.

Proper citation: RRID:Addgene_40728 Copy   

•   Source: Addgene

http://www.addgene.org/40999

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20733051
Comments: Venus YFP-3xFlag was inserted via BstXI-XhoI sites (to create pcDNA5/FRT/TO-Venus-Flag, Addgene Plasmid# 40998). The plasmid was then converted to a Gateway destination vector by inserting Gateway cassette C1, containing recombination sites attR1 and attR2, CmR, and ccdB, into the Afl II site (blunt ended). This plasmid can be used to place genes into the pcDNA5/FRT/TO-Venus-Flag construct by Gateway reaction, to make tetracycline-inducible stable cell lines. For subcloning, the following restriction sites are present: Between the CMV promoter and the Gateway cassette: PmeI (non-unique) Between the Gateway cassette and Venus: HindIII, Asp718I, KpnI, BamHI (non-unique, also in GW casette), and BstXI Between Venus and BGHpA: XhoI, Eco0109I, ApaI, and PmeI (non-unique)

Proper citation: RRID:Addgene_40999 Copy   

•   Source: Addgene

http://www.addgene.org/41390

Vector Backbone Description: Backbone Size:9260; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Plasmid for constitutive lentiviral expression. Alternate plasmid names: pLX305, SV40-puro; PGK-gateway-no tag

Proper citation: RRID:Addgene_41390 Copy   

•   Source: Addgene

http://www.addgene.org/41393

Vector Backbone Description: Backbone Size:9354; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The plasmid expresses rtTA-VP16-2A-puro from the PGK promoter, so it can be used as an 'all-in-one' dox on system. Alternate plasid names: pLIX_401 TRE-gateway; PGK-rtTA-2A-puro

Proper citation: RRID:Addgene_41393 Copy   

•   Source: Addgene

http://www.addgene.org/41439

Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.

Proper citation: RRID:Addgene_41439 Copy   

•   Source: Addgene

http://www.addgene.org/31221

Species: Homo sapiens
Genetic Insert: CD4-tdGFP
Vector Backbone Description: Backbone Size:10193; Vector Backbone:pAPIC-HIH; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21606367
Comments: Gateway destination vector for cloning any enhancer to drive expression of membrane marker CD4-tdGFP The depositing laboratory recommends growing bacteria either on LB plates with 80 ug/ml Carbenicillin or in LB liquid media with 60 ug/ml Carbenicillin (a semi-synthetic ampicillin analog)

Proper citation: RRID:Addgene_31221 Copy   

•   Source: Addgene

http://www.addgene.org/32190

Genetic Insert: TALE Nuclease, NG for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791

Proper citation: RRID:Addgene_32190 Copy   

•   Source: Addgene

http://www.addgene.org/32187

Genetic Insert: TALE Transcriptional Activator Backbone, NI for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791

Proper citation: RRID:Addgene_32187 Copy   

•   Source: Addgene

http://www.addgene.org/32185

Genetic Insert: TALE Transcriptional Activator Backbone, NN for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791

Proper citation: RRID:Addgene_32185 Copy   

•   Source: Addgene

http://www.addgene.org/32318

Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.

Proper citation: RRID:Addgene_32318 Copy   

•   Source: Addgene

http://www.addgene.org/32484

Vector Backbone Description: Backbone Marker:Mo Bi Tec; Vector Backbone:pET01; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18930792

Proper citation: RRID:Addgene_32484 Copy   

•   Source: Addgene

http://www.addgene.org/32684

Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was assembled and may contain minor discrepancies, which should not affect the function of the plasmid.

Proper citation: RRID:Addgene_32684 Copy   

•   Source: Addgene

http://www.addgene.org/32682

Vector Backbone Description: Backbone Size:8453; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658

Proper citation: RRID:Addgene_32682 Copy   

•   Source: Addgene

http://www.addgene.org/32685

Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was digitally assembled and may contain minor discrepancies, which should not effect the function of the plasmid.

Proper citation: RRID:Addgene_32685 Copy   

•   Source: Addgene

http://www.addgene.org/32671

Vector Backbone Description: Backbone Size:4427; Vector Backbone:pACAGW-ChR2-Venus-AAV; Vector Types:AAV, aav; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21772812

Proper citation: RRID:Addgene_32671 Copy   

•   Source: Addgene

http://www.addgene.org/45443

Vector Backbone Description: Backbone Marker:Connie Cepko lab (Harvard Medical School, Boston, MA); Backbone Size:7349; Vector Backbone:pEF-GFP (Addgene Plasmid #11154); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18754012
Comments: Alternate plasmid name: pEF1α-RfC.1-IRES-GFP To construct this vector, the depositing lab used EcoRI and BsrGI to replace the EGFP in pEF–GFP (Addgene plasmid 11154) with the IRES-GFP fragment from plasmid pBMN-I-GFP (Addgene plasmid 1736), creating intermediate vector pEF1α-IRES-GFP. Plasmid pEF1α-IRES-GFP was then converted to a Gateway-enabled destination vector with the Gateway Vector Conversion System (Invitrogen) by digesting with EcoRI, blunting the ends, and inserting Reading Frame Cassette C (RfC.1) gateway cassette.

Proper citation: RRID:Addgene_45443 Copy   

•   Source: Addgene