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Vector Backbone Description: Backbone Size:5769; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal Halotag tag at Bgl II/Stu I restriction site
Proper citation: RRID:Addgene_186377 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197790 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197798 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197794 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197791 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197844 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197842 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197840 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197789 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197785 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197804 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197801 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197802 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197809 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197808 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:7673; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified by insertion of C terminal Venus tag at EcoR V restriction site
Proper citation: RRID:Addgene_186409 Copy
Genetic Insert: mEos3.2-cat
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26145755
Proper citation: RRID:Addgene_78100 Copy
Species: synthetic
Genetic Insert: Gateway cloning cassette
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pMalC2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27105777
Proper citation: RRID:Addgene_78572 Copy
Vector Backbone Description: Backbone Marker:Wellcome Trust Sanger; Vector Backbone:pMCS-AAT-PB:PGKpuro∆tk; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38986614
Comments: Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.
Proper citation: RRID:Addgene_218893 Copy
Vector Backbone Description: Vector Backbone:pAC1801_pmax-dCasRx (Addgene #118634); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38917795
Comments: This was the destination vector used for generating the dCasRx-SF library for screening by co-transfection with the luciferase reporters. We have found that cloning dCasRx-SFs into the pXR002 backbone gave greater transfection efficiency for endogenous applications. We recommend using the EF1a-dCasRx-RBM25 vector (Addgene #221001) for endogenous targeting.
Proper citation: RRID:Addgene_221003 Copy
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