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Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS425; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14370 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS426; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14420 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS303; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14398 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS306; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14403 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14402 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS304; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14400 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14409 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS414; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14407 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS425; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14417 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS424; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14415 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS423; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14413 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14410 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS426; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14419 Copy
Species: cloning vector
Genetic Insert: pWS-TK2
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18546598
Comments: The deposited sequence file of pWS-TK2 is from compilation of previous plasmids, and only part of it has been re-sequenced. There is a gap between author's sequence and Addgene sequence that will NOT affect the use of this plasmid.
Please also note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.
pWS-TK2 is designed to have two variants of thymidine kinase gene from herpes virus.
Proper citation: RRID:Addgene_20348 Copy
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:9370; Vector Backbone:pBluescript KS; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19252478
Proper citation: RRID:Addgene_20909 Copy
Vector Backbone Description: Backbone Marker:ATCC(pUC18) and Christensen and Quail(pAHC25); Backbone Size:4930; Vector Backbone:pUC18 and pAHC25; Vector Types:Plant expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_33297 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi14024 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34862 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4391 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: There is a 461 bp deletion in the middle of the left recombination region. This deletion does not appear to adversely affect the ability to insert transgenes into the ttTi4391 site.
Proper citation: RRID:Addgene_34861 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10816 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34864 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Neomycin resistance vector for drug selection in worms.
This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34919 Copy
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