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Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_198637 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.
Proper citation: RRID:Addgene_199082 Copy
Vector Backbone Description: Backbone Size:8409; Vector Backbone:pLX_TRC317 (https://portals.broadinstitute.org/gpp/public/vector/details?vector=pLX_TRC317); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35085500
Proper citation: RRID:Addgene_178534 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco-aphA
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81013 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco-PMKco
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81016 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81017 Copy
Species: Synthetic
Genetic Insert: sgRNA-scaffold only
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:2125; Vector Backbone:pMD19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35969224
Proper citation: RRID:Addgene_190794 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28060929
Proper citation: RRID:Addgene_190226 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:4677; Vector Backbone:pDEST-pSP172BSSPE-Swa1::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified by insertion of N terminal HA tag at Stu I restriction site
Proper citation: RRID:Addgene_186404 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28060929
Proper citation: RRID:Addgene_190238 Copy
Vector Backbone Description: Backbone Marker:Stephen Elledge (Addgene #44012); Backbone Size:11874; Vector Backbone:pINDUCER20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: C terminal 3x Flag on backbone
Proper citation: RRID:Addgene_190028 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197841 Copy
Vector Backbone Description: Backbone Size:10157; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201122 Copy
Vector Backbone Description: Backbone Size:9859; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201136 Copy
Vector Backbone Description: Backbone Size:10258; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201141 Copy
Vector Backbone Description: Backbone Size:10492; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201143 Copy
Vector Backbone Description: Backbone Size:9841; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201142 Copy
Vector Backbone Description: Backbone Size:9954; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201123 Copy
Vector Backbone Description: Backbone Size:5829; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal miRFP713 tag at Bgl II/Stu I restriction site
Proper citation: RRID:Addgene_186381 Copy
Vector Backbone Description: Backbone Size:5583; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal mVenus tag at EcoR V/Avr II restriction site
Proper citation: RRID:Addgene_186394 Copy
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