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Species: E. coli
Genetic Insert: zapB
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18394147
Proper citation: RRID:Addgene_31398 Copy
Species: E. coli
Genetic Insert: lacZ ORF with short 5'UTR
Vector Backbone Description: Backbone Size:13150; Vector Backbone:pMS26; Vector Types:Bacterial Expression, bacterial gene addition; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22123741
Comments: Amplification and sequencing primers for verifying insertion into attTn7:
glmS-proximal (Tn7R side)
5’-AATCTGTAACGTTCCGGGTTC-3’ OR
5'-GATGCTGGTGGCGAAGCTGT-3'
pstS-proximal (Tn7L side)
5’-CATTAATAACGAAGAGATGAC-3’ OR
5'-GATGACGGTTTGTCACATGGA-3'
Proper citation: RRID:Addgene_32296 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_32408 Copy
Species: E. coli
Genetic Insert: LacO (256 copies)
Vector Backbone Description: Backbone Marker:Addgene plasmid #33143; Vector Backbone:pVS1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18614049
Comments: Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable.
For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142).
pVS2 contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 [Addgene #33143] is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]).
pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.
Proper citation: RRID:Addgene_33144 Copy
Species: E. coli
Genetic Insert: BirA::GFP
Vector Backbone Description: Backbone Size:2884; Vector Backbone:pWormgate2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22219512
Proper citation: RRID:Addgene_45100 Copy
Species: e. coli
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4102; Vector Backbone:pBAD/His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45397 Copy
Species: e. coli
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45392 Copy
Species: e. coli
Genetic Insert: tet repressor
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45391 Copy
Species: e. coli
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45394 Copy
Species: E. coli
Genetic Insert: beta-Galactosidase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_45964 Copy
Species: E. coli
Genetic Insert: beta-Galactosidase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_45967 Copy
Species: E. coli
Genetic Insert: ECK120033127 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46011 Copy
Species: E. coli
Genetic Insert: ECK120010800 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46010 Copy
Species: E. coli
Genetic Insert: BBa_B0010 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46003 Copy
Species: E. coli
Genetic Insert: ECK125108723 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46008 Copy
Species: E. coli
Genetic Insert: ECK120010783 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46009 Copy
Species: E. coli
Genetic Insert: ECK120015170 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46006 Copy
Species: E. coli
Genetic Insert: Streptavidin-Dead Aspartate Loop
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056174
Proper citation: RRID:Addgene_46368 Copy
Species: E. coli
Genetic Insert: exonucleaseIII E. coli
Vector Backbone Description: Backbone Marker:Homemade; Backbone Size:6686; Vector Backbone:pMA2780; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23721969
Proper citation: RRID:Addgene_46884 Copy
Species: E. coli
Genetic Insert: pFLIPmal-40uDelta1V
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18522753
Comments: W230A mutation, which we introduced to change the affinity.
Proper citation: RRID:Addgene_18854 Copy
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