Plasmids   Select Another Resource Report Type

http://www.addgene.org/14477

Species: E. coli
Genetic Insert: FLIPmal
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRT100/pPZP312; Vector Types:Plant Expression, Plant Nanosensor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16935985
Comments: For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html FLIPmalEW340A with promoter from pRT100.

Proper citation: RRID:Addgene_14477 Copy   

•   Source: Addgene

http://www.addgene.org/14482

Species: E. coli
Genetic Insert: FLIPmal
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12097642
Comments: For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

Proper citation: RRID:Addgene_14482 Copy   

•   Source: Addgene

http://www.addgene.org/14480

Species: E. coli
Genetic Insert: FLIPmal
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12097642
Comments: For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

Proper citation: RRID:Addgene_14480 Copy   

•   Source: Addgene

http://www.addgene.org/145376

Species: E. coli
Genetic Insert: AcpS
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32401365

Proper citation: RRID:Addgene_145376 Copy   

•   Source: Addgene

http://www.addgene.org/20218

Species: E. coli
Genetic Insert: 2xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA tag and two repeats of DHFR

Proper citation: RRID:Addgene_20218 Copy   

•   Source: Addgene

http://www.addgene.org/20215

Species: E. coli
Genetic Insert: 2xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807

Proper citation: RRID:Addgene_20215 Copy   

•   Source: Addgene

http://www.addgene.org/20219

Species: E. coli
Genetic Insert: 3xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA tag and three repeats of DHFR

Proper citation: RRID:Addgene_20219 Copy   

•   Source: Addgene

http://www.addgene.org/20856

Species: E. Coli
Genetic Insert: BirA-ER
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18425138
Comments: Please email [email protected] with the intended use and/or targets for this plasmid.

Proper citation: RRID:Addgene_20856 Copy   

•   Source: Addgene

http://www.addgene.org/33259

Species: E. coli
Genetic Insert: LexA::VP16-SV40 3'UTR
Vector Backbone Description: Vector Backbone:pCasPeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060186
Comments: A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006). The PCR fragment was then subcloned into the XbaI site of the pBS vector. LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter.

Proper citation: RRID:Addgene_33259 Copy   

•   Source: Addgene

http://www.addgene.org/33376

Species: E. coli
Genetic Insert: phnD based Phosphonate Biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22019591
Comments: The EcPhnD-cpGFP insertion variants were constructed by overlap PCR using the synthetic EcPhnD sequence and the cpGFP146 variant from GCaMP2. Detailed sequences are provided in attached sheets. The linker regions were mutated by single-stranded template mutagenesis

Proper citation: RRID:Addgene_33376 Copy   

•   Source: Addgene

http://www.addgene.org/33372

Species: E. coli
Genetic Insert: MBP based Maltose Biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21989929
Comments: The MBP-cpGFP insertion variant was constructed by overlap PCR using the wild-type MBP sequence from the pMalE-p4E vector (New England Biolabs) and the cpGFP146 variant from GCaMP2 into pRSET-A between BamHI and EcoRI sites. The N-terminal periplasmic leader sequence of MBP was excluded, and a BamHI site (encoding Gly-Ser) was included at the 5′-end. At the C-terminus, an additional His6Gly tag was included after the terminus of MBP (a cloning oversight). An EcoRI site was included after the stop codon.

Proper citation: RRID:Addgene_33372 Copy   

•   Source: Addgene

http://www.addgene.org/34627

Species: E. coli
Genetic Insert: EF-Sep
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21868676
Comments: To construct pMAL-EFSep the gene encoding EF-Sep, was cloned between the NdeI and BamHI sites in the pET20b plasmid (Novaven) to add a C-terminal His6 tag. This fusion construct was then PCR-amplified using primers adding MfeI and PstI restriction sites. The PCR product was cloned in-frame between EcoRI and PstI in pMALc2x (New England Biolabs) to add an N-terminal maltose binding protein (MBP) tag. To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.

Proper citation: RRID:Addgene_34627 Copy   

•   Source: Addgene

http://www.addgene.org/35041

Species: e. coli
Genetic Insert: cytochrome b562
Vector Backbone Description: Vector Backbone:pUC 18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1761034

Proper citation: RRID:Addgene_35041 Copy   

•   Source: Addgene

http://www.addgene.org/40108

Species: E. Coli
Genetic Insert: Bistable switch using dynamic degradation
Vector Backbone Description: Backbone Size:3460; Vector Backbone:psc101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22776405

Proper citation: RRID:Addgene_40108 Copy   

•   Source: Addgene

http://www.addgene.org/40127

Species: E. Coli
Genetic Insert: CI inducible sfGFP
Vector Backbone Description: Backbone Size:2097; Vector Backbone:p15A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22776405

Proper citation: RRID:Addgene_40127 Copy   

•   Source: Addgene

http://www.addgene.org/40126

Species: E. Coli
Genetic Insert: Lac inducible lambda repressor
Vector Backbone Description: Backbone Size:4147; Vector Backbone:ColE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22776405

Proper citation: RRID:Addgene_40126 Copy   

•   Source: Addgene

http://www.addgene.org/40190

Species: E. coli
Genetic Insert: EcoRI endonuclease & EcoRI methylase
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pSCC3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2745418
Comments: pSCC3 plasmid derivation is described in King, K. et al. (1989) J.Biol.Chem. 264, 11807-11815. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_40190 Copy   

•   Source: Addgene

http://www.addgene.org/40942

Species: E. coli
Genetic Insert: GFP-LacI**
Vector Backbone Description: Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:5650; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21724830
Comments: The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. Alternate plasmid name: pAT123LacI2G(4mutations)

Proper citation: RRID:Addgene_40942 Copy   

•   Source: Addgene

http://www.addgene.org/41438

Species: E. coli
Genetic Insert: LexAp65
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR P4r-P3r; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_41438 Copy   

•   Source: Addgene

http://www.addgene.org/31340

Species: E. coli
Genetic Insert: E. coli Initiation Factor 3
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET21c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12095986

Proper citation: RRID:Addgene_31340 Copy   

•   Source: Addgene