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Species: Caenorhabditis elegans
Genetic Insert: K+ channel for Habituation to Tap subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19387491
Proper citation: RRID:Addgene_113054 Copy
Species: Caenorhabditis elegans
Genetic Insert: MiRP K+ channel accessory Subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17604313
Comments: This plasmid contains an N214K mutation compared to the reference sequence NP_495263.2 that is not of concern for function.
Proper citation: RRID:Addgene_113059 Copy
Species: Caenorhabditis elegans
Genetic Insert: K+ voltage sensitive channel subunit 1.2
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17488718
Comments: This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.
Proper citation: RRID:Addgene_113069 Copy
Species: Caenorhabditis elegans
Genetic Insert: lis-1
Vector Backbone Description: Backbone Marker:L4440 is available at Addgene; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: Full length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11336 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_17254 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-no stop in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20169625
Proper citation: RRID:Addgene_17250 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 intron:pie-1 promoter in PDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_17246 Copy
Species: Caenorhabditis elegans
Genetic Insert: tbb-2 3'UTR in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_17249 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::mScarlet
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mScarlet. Use as a co-injection marker. pGLOW30 mScarlet fluorescence is brighter than pCFJ90 mCherry fluorescence in the pharynx. Inject at 2.5 ng/uL. Made by George Huang (Glow Worms '20).
Proper citation: RRID:Addgene_172697 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prgef-1
Vector Backbone Description: Vector Backbone:Gateway final clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28767038
Comments: Please note that the Addgene NGS sequence differs from the depositor's annotated sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_172696 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3
Vector Backbone Description: Vector Backbone:Gateway final clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28767038
Comments: Please note that the Addgene NGS sequence differs from the depositor's annotated sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_172694 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::AID::GFP::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173743 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173746 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2675; Vector Backbone:pHSG298; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173740 Copy
Species: Caenorhabditis elegans
Genetic Insert: rps-0 promoter
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2675; Vector Backbone:pHSG298; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173739 Copy
Species: Caenorhabditis elegans
Genetic Insert: hsp-16.48 promoter
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2675; Vector Backbone:pHSG298; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173736 Copy
Species: Caenorhabditis elegans
Genetic Insert: elt-2 promoter
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2675; Vector Backbone:pHSG298; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173737 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pelt-2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173749 Copy
Species: Caenorhabditis elegans
Genetic Insert: (empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173754 Copy
Species: Caenorhabditis elegans
Genetic Insert: Phsp-16.48::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173750 Copy
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