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Genetic Insert: MevTo-BBa1002-pTrc-Mkco
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81017 Copy
Vector Backbone Description: Backbone Size:9748; Vector Backbone:plex_306; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201135 Copy
Vector Backbone Description: Backbone Size:10986; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201125 Copy
Vector Backbone Description: Backbone Size:10580; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201126 Copy
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_198637 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.
Proper citation: RRID:Addgene_199082 Copy
Vector Backbone Description: Backbone Size:15069; Vector Backbone:Lenti-CRISPR; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27458201
Proper citation: RRID:Addgene_85402 Copy
Genetic Insert: CreER
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pT3; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28009940
Proper citation: RRID:Addgene_85577 Copy
Genetic Insert: CreER
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pT3; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28009940
Proper citation: RRID:Addgene_85578 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85723 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85720 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85724 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85728 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85729 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85719 Copy
Vector Backbone Description: Backbone Size:9127; Vector Backbone:LentiGuide; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27458201
Proper citation: RRID:Addgene_85745 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86381 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster ssp3 enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86382 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3477; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86380 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster ham enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86383 Copy
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