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pPV454
 
Resource Report
Resource Website 		RRID:Addgene_42922 Ce-age-1 promoter (873 bp)::Ce-age-1 cDNA (3549 bp)::Ce-unc-54 terminator (765 bp) Caenorhabditis elegans Ampicillin and Kanamycin PMID:22701676 The construct Ce-age-1p::Ce-age-1(3,549bp)::Ce-unc-54t, contained in plasmid pPV454, was assembled by overlap extension PCR. An 873 bp segment of Ce-age-1 5′ region was amplified from genomic DNA and fused to 2,376 bp of the 5′ region of Ce-age-1, amplified from cDNA, using the overlapping primers Ceage1p-cDNA-F and -R (below). This was subsequently fused to the remainder of the Ce-age-1 cDNA with the plasmid Pdpy-30-age-1, a gift from Yuichi Iino (University of Tokyo, Tokyo, Japan), as template and using the overlapping primers Ceage1-Cat-F and -R (below). Finally, the promoter and full-length 3,549 bp Ce-age-1 cDNA were fused to 765 bp of the Ce-unc-54 3′ region (terminator), amplified from Pdpy-30-age-1 template, using the overlapping primers Ceage1-unc54-F and -R (below). The PCR product was T/A cloned into the pCR4-TOPO vector (Life Technologies) and the insert fully sequenced. Ceage1p-cDNA-F caagctttcattttaagattttaagATGTCTATGGGACGAAGCCCCTC Ceage1p-cDNA-R GAGGGGCTTCGTCCCATAGACATcttaaaatcttaaaatgaaagcttg Ceage1-Cat-F CCTCCGTGGAAATGAAGAGCACatcaagatcatcacccgacaag Ceage1-Cat-R cttgtcgggtgatgatcttgatGTGCTCTTCATTTCCACGGAGG Ceage1-unc54-F CCACGCAGTCAAACACTACTGAgataagagctccgcatcggccg Ceage1-unc54-R cggccgatgcggagctcttatcTCAGTAGTGTTTGACTGCGTGG Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-AGAC
 
Resource Report
Resource Website 		RRID:Addgene_42996 I-AGAC Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-AGAG
 
Resource Report
Resource Website 		RRID:Addgene_42997 I-AGAG Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACTA
 
Resource Report
Resource Website 		RRID:Addgene_42991 I-ACTA Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACTC
 
Resource Report
Resource Website 		RRID:Addgene_42992 I-ACTC Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACTG
 
Resource Report
Resource Website 		RRID:Addgene_42993 I-ACTG Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACTT
 
Resource Report
Resource Website 		RRID:Addgene_42994 I-ACTT Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACCT
 
Resource Report
Resource Website 		RRID:Addgene_42986 I-ACCT Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACGG
 
Resource Report
Resource Website 		RRID:Addgene_42989 I-ACGG Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACAT
 
Resource Report
Resource Website 		RRID:Addgene_42982 I-ACAT Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACCC
 
Resource Report
Resource Website 		RRID:Addgene_42984 I-ACCC Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-AATA
 
Resource Report
Resource Website 		RRID:Addgene_42975 I-AATA Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-AATC
 
Resource Report
Resource Website 		RRID:Addgene_42976 I-AATC Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-ACAA
 
Resource Report
Resource Website 		RRID:Addgene_42979 I-ACAA Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-AAAA
 
Resource Report
Resource Website 		RRID:Addgene_42963 I-AAAA Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-AAAC
 
Resource Report
Resource Website 		RRID:Addgene_42964 I-AAAC Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-AAAG
 
Resource Report
Resource Website 		RRID:Addgene_42965 I-AAAG Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:46 0
I-AGCG
 
Resource Report
Resource Website 		RRID:Addgene_43001 I-AGCG Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:47 0
I-AGCT
 
Resource Report
Resource Website 		RRID:Addgene_43002 I-AGCT Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:47 0
I-AGGA
 
Resource Report
Resource Website 		RRID:Addgene_43003 I-AGGA Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:54:47 0

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