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Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19678 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R4-R3)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19677 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7624; Vector Backbone:pCDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19702 Copy
Genetic Insert: pgLAP3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8325; Vector Backbone:pEF5/FRT-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19704 Copy
Genetic Insert: pgLAP5
Vector Backbone Description: Backbone Size:8365; Vector Backbone:pEF5/FRT-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19706 Copy
Genetic Insert: pgLAP4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7638; Vector Backbone:pEF5/FRT-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19705 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:E.coli bacterial strain; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19633652
Comments: MG1655, bla, bio-, lambda-Red1, mutS–, cmR
From the paper: mutS in EcNR1 was replaced with the chloramphenicol resistance gene (cmR cassette).
Proper citation: RRID:Addgene_26931 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
Vector Backbone Description: Backbone Size:17037; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: Second Generation pie-1 vector for expressing ORFs in the germline
Proper citation: RRID:Addgene_26955 Copy
Genetic Insert: pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:12296; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: For expressing GFP-ORF fusions in the germline. This vector does not contain unc-119 sequences and therefore must be co-injected with a marker (typically we use pRF4 in complex array injections - Kelly et al., 1997)
Proper citation: RRID:Addgene_26952 Copy
Genetic Insert: pUC/ori, bla
Vector Backbone Description: Backbone Marker:Gateway(R) (Invitrogen); Backbone Size:3012; Vector Backbone:pDONRP2R-P3; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21203517
Proper citation: RRID:Addgene_27367 Copy
Genetic Insert: pRARE2 + pBADmod1-linker2-gamS plasmid
Vector Backbone Description: Vector Backbone:Strain; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35034449
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.07.459228v1 for bioRxiv preprint.
Primers for recB deletion verification:
Foward - tattttccagtcgtgaaagc
Reverse - ttgctgatttcttccatcag
Primers for GamS plasmid verification:
Forward - aattatgacaacttgacggctacatcattc
Reverse - cgttcaccgacaaacaaca
Proper citation: RRID:Addgene_176585 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161880 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161883 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161887 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161888 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161878 Copy
Vector Backbone Description: Backbone Size:10000; Vector Backbone:MA1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24388174
Proper citation: RRID:Addgene_161783 Copy
Vector Backbone Description: Backbone Size:10000; Vector Backbone:MA1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24388174
Proper citation: RRID:Addgene_161786 Copy
Vector Backbone Description: Backbone Size:10000; Vector Backbone:MA1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24388174
Proper citation: RRID:Addgene_161785 Copy
Vector Backbone Description: Vector Backbone:pET43.1a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_161890 Copy
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