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Species: E. coli
Genetic Insert: LetB
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139922 Copy
Species: E. coli
Genetic Insert: LetB
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139888 Copy
Species: E. coli
Genetic Insert: LetB
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139921 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139927 Copy
Species: E. coli
Genetic Insert: LetB
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139924 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139928 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139892 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139890 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139891 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139896 Copy
Species: E. coli
Genetic Insert: Colicin Ia + Ia immunity protein
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:PUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19919671
Comments: The original clone had some mutations that had been introduced during the PCR cloning of the colicin operon (into another vector). Here, the operon was moved into pUC19 and the mutations were corrected, so that the sequence is the same as the one in GenBank (M13819.1)
This plasmid contains Colicin Ia + Ia immunity protein, each with their own promoters; colicin production can be induced with mitomycin C.
The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other.
Proper citation: RRID:Addgene_27125 Copy
Species: E. coli
Genetic Insert: grpE
Vector Backbone Description: Backbone Size:4493; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27391 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ, groESL
Vector Backbone Description: Backbone Size:9177; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Between depositor sequence and Addgene sequencing results, there is a mismatch at position 7284 and an insertion at position 7360. These are in vector backbone and do not alter expression of the inserts.
Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27395 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ, GroESL
Vector Backbone Description: Backbone Size:9203; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
single nt insertion at position 1726.
Proper citation: RRID:Addgene_27396 Copy
Species: E. coli
Genetic Insert: grpE, clpB
Vector Backbone Description: Backbone Size:7194; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27393 Copy
Species: E. coli
Genetic Insert: MutS
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9308969
Comments: pSL6 contains a ClaI–BamHI fragment of pMS312 carrying E. coli mutS and the KanR-conferring HindIII–BamHI fragment of pKC31
Proper citation: RRID:Addgene_28029 Copy
Species: E. Coli
Genetic Insert: FLIPglu-2µΔ13V
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20854260
Proper citation: RRID:Addgene_28000 Copy
Species: E. coli
Genetic Insert: acpS
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: 1% arabinose induction at 37ºC.
Proper citation: RRID:Addgene_141140 Copy
Species: E. coli
Genetic Insert: FLIPmal
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12097642
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
Proper citation: RRID:Addgene_14479 Copy
Species: E. coli
Genetic Insert: FLIPmal
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRT100/pPZP312; Vector Types:Plant Expression, Plant Nanosensor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16935985
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
FLIPmalEW230A with promoter from pRT100.
Proper citation: RRID:Addgene_14476 Copy
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