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Species: Caenorhabditis elegans
Genetic Insert: Ce-age-1 promoter (873 bp)::Ce-age-1 cDNA (3549 bp)::Ce-unc-54 terminator (765 bp)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22701676
Comments: The construct Ce-age-1p::Ce-age-1(3,549bp)::Ce-unc-54t, contained in plasmid pPV454, was assembled by overlap extension PCR. An 873 bp segment of Ce-age-1 5′ region was amplified from genomic DNA and fused to 2,376 bp of the 5′ region of Ce-age-1, amplified from cDNA, using the overlapping primers Ceage1p-cDNA-F and -R (below). This was subsequently fused to the remainder of the Ce-age-1 cDNA with the plasmid Pdpy-30-age-1, a gift from Yuichi Iino (University of Tokyo, Tokyo, Japan), as template and using the overlapping primers Ceage1-Cat-F and -R (below). Finally, the promoter and full-length 3,549 bp Ce-age-1 cDNA were fused to 765 bp of the Ce-unc-54 3′ region (terminator), amplified from Pdpy-30-age-1 template, using the overlapping primers Ceage1-unc54-F and -R (below). The PCR product was T/A cloned into the pCR4-TOPO vector (Life Technologies) and the insert fully sequenced.
Ceage1p-cDNA-F caagctttcattttaagattttaagATGTCTATGGGACGAAGCCCCTC
Ceage1p-cDNA-R GAGGGGCTTCGTCCCATAGACATcttaaaatcttaaaatgaaagcttg
Ceage1-Cat-F CCTCCGTGGAAATGAAGAGCACatcaagatcatcacccgacaag
Ceage1-Cat-R cttgtcgggtgatgatcttgatGTGCTCTTCATTTCCACGGAGG
Ceage1-unc54-F CCACGCAGTCAAACACTACTGAgataagagctccgcatcggccg
Ceage1-unc54-R cggccgatgcggagctcttatcTCAGTAGTGTTTGACTGCGTGG
Proper citation: RRID:Addgene_42922 Copy
Species: Synthetic
Genetic Insert: I-AGAC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42996 Copy
Species: Synthetic
Genetic Insert: I-AGAG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42997 Copy
Species: Synthetic
Genetic Insert: I-ACTA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42991 Copy
Species: Synthetic
Genetic Insert: I-ACTC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42992 Copy
Species: Synthetic
Genetic Insert: I-ACTG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42993 Copy
Species: Synthetic
Genetic Insert: I-ACTT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42994 Copy
Species: Synthetic
Genetic Insert: I-ACCT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42986 Copy
Species: Synthetic
Genetic Insert: I-ACGG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42989 Copy
Species: Synthetic
Genetic Insert: I-ACAT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42982 Copy
Species: Synthetic
Genetic Insert: I-ACCC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42984 Copy
Species: Synthetic
Genetic Insert: I-AATA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42975 Copy
Species: Synthetic
Genetic Insert: I-AATC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42976 Copy
Species: Synthetic
Genetic Insert: I-ACAA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42979 Copy
Species: Synthetic
Genetic Insert: I-AAAA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42963 Copy
Species: Synthetic
Genetic Insert: I-AAAC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42964 Copy
Species: Synthetic
Genetic Insert: I-AAAG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42965 Copy
Species: Synthetic
Genetic Insert: I-AGCG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43001 Copy
Species: Synthetic
Genetic Insert: I-AGCT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43002 Copy
Species: Synthetic
Genetic Insert: I-AGGA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43003 Copy
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