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Species: S. mediterranea
Genetic Insert: Protein arginine methyltransferase 1 homolog
Vector Backbone Description: Backbone Marker:Jim Collins, Newmark lab; Vector Backbone:pJC53.2; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22318224
Comments: There are several mismatches between Addgene's quality control sequence and the available NCBI sequence. Depositing lab believes these mismatches will not affect plasmid function.
Proper citation: RRID:Addgene_35693 Copy
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pKD4; Vector Types:E.coli genomic integration; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23799955
Comments: This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between rbsAR homologous arms and integrated at the rbsAR locus.
Proper citation: RRID:Addgene_46768 Copy
Species: Synthetic
Genetic Insert: mCherryS131C
Vector Backbone Description: Backbone Marker:QIAGEN; Backbone Size:3439; Vector Backbone:pQE-9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21696135
Comments: This plasmid must be grown under Ampicillin (100ug/ml) and Kanamycin (50ug/ml) selection in order to retain both the mCherryS131C plasmid and the pREP4 helper plasmid.
Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results for recommended primers.
The S131C mutation in this plasmid is S136C, when compared to the starting Met of wild-type mCherry.
Proper citation: RRID:Addgene_48732 Copy
Vector Backbone Description: Backbone Marker:Mermelstein et al. 1992; Backbone Size:4500; Vector Backbone:pIMP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:14766568
Comments: For more information about this plasmid, see the associated publication (PMID: 14766568), as well as the publication describing its original construction: Mai, Lorenz, and Wiegel, Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance. FEMS Microbiol Lett. 1997 DOI: 10.1111/j.1574-6968.1997.tb10283.x ( http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1997.tb10283.x/abstract )
Proper citation: RRID:Addgene_51054 Copy
Genetic Insert: GFP4m
Vector Backbone Description: Vector Backbone:p2attNG; Vector Types:Mammalian Expression, Human Targeting; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24304893
Proper citation: RRID:Addgene_51546 Copy
Species: S. pyogenes
Genetic Insert: Cas9 nickase
Vector Backbone Description: Backbone Marker:Thermo-Fisher; Backbone Size:3900; Vector Backbone:PCR2-1 TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Comments: The poly A tail included in the sequence is sufficient for purification of full length transcript using an oligo dT column. This decreases the amount of incomplete transcripts (and therefore decreases truncated Cas9 proteins).
Proper citation: RRID:Addgene_71311 Copy
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71739 Copy
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71736 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: response regulator in two-component regulatory system with EvgS
Vector Backbone Description: Backbone Size:3851; Vector Backbone:pDRIVE-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: TA cloning product with 286 bp 5'-UTR including EvgA binding site.
Proper citation: RRID:Addgene_72467 Copy
Species: Acetobacter aceti 1023
Genetic Insert: DNA mismatch repair protein MutS (partial gene)
Vector Backbone Description: Backbone Size:3851; Vector Backbone:pDRIVE-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Used for nucleotide quantitation in RT-PCR and similar experiments for Acetobacter aceti 1023.
Based on method described in M. Ishikawa, A. Okamoto-Kainuma, T. Jochi, I. Suzuki, K. Matsui, T Kaga, Y. Koizumi (2009) Cloning and characterization of grpE in Acetobacter pasteurianus NBRC 3283. J. Biosci. Bioeng. 109(1), 25–31[doi: 10.1016/j.jbiosc.2009.07.008]
Proper citation: RRID:Addgene_72437 Copy
Species: Gallus gallus
Genetic Insert: CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4339; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24685175
Proper citation: RRID:Addgene_69138 Copy
Species: Synthetic
Genetic Insert: Tol2-kanaR-cryaa-CFP
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26818072
Proper citation: RRID:Addgene_74151 Copy
Species: Mus musculus
Genetic Insert: TCR beta cDNA clone HSV2.3
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:3947; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:11940116
Comments: This plasmid contains the TCR beta chain cDNA from the HSV2.3 clone and was used to create the transgenic construct p3A9cbetaTCR.HSV2.3
(Addgene plasmid #69594).
Proper citation: RRID:Addgene_74077 Copy
Species: Synthetic
Genetic Insert: aphII
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-54, 1995; Vector Backbone:pBSL15; Vector Types:Bacterial vector based on pMB1 replicon; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26921431
Proper citation: RRID:Addgene_74108 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68029 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68033 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68032 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68031 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68030 Copy
Species: Synthetic
Genetic Insert: Staphylococcus aureus guide RNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_64710 Copy
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