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Species: Rattus norvegicus
Genetic Insert: ACTN3
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 455–473 from the start sequence of rat α-actinin-3 (the sense chain was 5’-aacgagaagctgatggaggttcaagagacctccatcagcttctcgtttttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52678 Copy
Species: Rattus norvegicus
Genetic Insert: hM4Di
Vector Backbone Description: Backbone Marker:Dr. Rachael Neve, McLean Hospital, Boston, MA; Backbone Size:5307; Vector Backbone:pHSV-PrPUC; Vector Types:Mammalian Expression, HSV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131952
Proper citation: RRID:Addgene_53327 Copy
Species: Rattus norvegicus
Genetic Insert: SNAP-25B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53235 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53237 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53238 Copy
Species: Rattus norvegicus
Genetic Insert: SNAP-25B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Comments: This SNAP25B double mutant is resistant to both botulinum toxin C1 (A199R) and botulinum toxin E (D179K)
Proper citation: RRID:Addgene_53259 Copy
Species: Rattus norvegicus
Genetic Insert: Tyrosinase gRNA
Vector Backbone Description: Backbone Size:2147; Vector Backbone:DR274; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24967838
Comments: Targets a mutation 896G>A in exon 2 of the Tyr gene resulting in an Arg299His missense mutation
Proper citation: RRID:Addgene_60966 Copy
Species: Rattus norvegicus
Genetic Insert: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA
Vector Backbone Description: Backbone Size:2147; Vector Backbone:DR274; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24967838
Comments: Targets an integrated 7,098-bp endogenous retroviral element (ERV) within the first intron of the Kit gene
Proper citation: RRID:Addgene_60969 Copy
Species: Rattus norvegicus
Genetic Insert: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA
Vector Backbone Description: Backbone Size:2147; Vector Backbone:DR274; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24967838
Comments: Targets an integrated 7,098-bp endogenous retroviral element (ERV) within the first intron of the Kit gene
Proper citation: RRID:Addgene_60971 Copy
Species: Rattus norvegicus
Genetic Insert: C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24463606
Proper citation: RRID:Addgene_61155 Copy
Species: Rattus norvegicus
Genetic Insert: alpha SSeCKS
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II SK(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Note: Addgene's quality control sequencing finds a single nucleotide mismatch in the alpha-SSeCKS 3' UTR
Proper citation: RRID:Addgene_61295 Copy
Species: Rattus norvegicus
Genetic Insert: Myosin Vb
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18311135
Proper citation: RRID:Addgene_110170 Copy
Species: Rattus norvegicus
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20628354
Proper citation: RRID:Addgene_110181 Copy
Species: Rattus norvegicus
Genetic Insert: intrabody against mouse interferon alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4888; Vector Backbone:pcDNA/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30990863
Proper citation: RRID:Addgene_110798 Copy
Species: Rattus norvegicus
Genetic Insert: ERK1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pMT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12656 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP7s
Vector Backbone Description: Backbone Marker:Derived in Kotton lab; Backbone Size:6111; Vector Backbone:pHAGE2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126690 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP7f
Vector Backbone Description: Backbone Marker:Derived in Kotton lab; Backbone Size:6111; Vector Backbone:pHAGE2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126691 Copy
Species: Rattus norvegicus
Genetic Insert: G protein alpha-s-miRFP670nano
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30655515
Proper citation: RRID:Addgene_127441 Copy
Species: Rattus norvegicus
Genetic Insert: calcium/calmodulin-dependent protein kinase II alpha
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31239443
Proper citation: RRID:Addgene_127392 Copy
Species: Rattus norvegicus
Genetic Insert: calcium/calmodulin-dependent protein kinase II alpha
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31239443
Proper citation: RRID:Addgene_127391 Copy
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